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微卫星DNA标记是近年来最受研究者青睐的分子标记之一。由于其具有高度多态性、共显性遗传、基因组中含量丰富且随机分布等特点,已被应用于种群分化研究、亲缘分析、基因连锁分析、进化以及生态学研究等许多领域。近年来,蟹类微卫星的研究报道日益增多。文中对蟹类微卫星分离方法、引物设计、遗传学特性以及在种群遗传、家系分析、遗传多样性评价等方面的最新研究进展进行综述,并分析微卫星分析中无效等位基因(null allele)、"结巴"带(stutter bands)和上游等位基因"扩增丢失"现象(upper allelic dropout)的产生原因以及对微卫星基因型判读带来的影响。
Abstract：In the last few years,microsatellites have become one of the most popular molecular markers used with applications in many different fields.Microsatellite DNA is among the most efficient class of molecular markers due to their hyper-variable and co-dominant nature with relatively high abundance and random distribution in the genome,and has been applied to a variety of fields including population differentiation,kinship analysis,linkage analysis,and evolutional and ecological studies.Recently,studies on microsatellites in crabs are increasing.In this article,we review the isolation methods,primer of design,developmental status and genetic characteristics of microsatellites,and their applications in studies on population study,pedigree analysis,assessment of genetic diversity,and analyze the causes resulting in null alleles,stutter bands,upper allelic dropouts and their effects on the genotyping of microsatellites.
[1]堵南山.世界食用蟹类[J].水产科技情报,1997,24(3):104-107.
[2]王青,孔晓瑜,于珊珊,等.十足目染色体研究进展[J].海洋科学,2005,29(6):60-65.
[3]Hines A H,Wolcott T G,Lipcius R N.Populations and communityecology of the blue crabs(Callinectes sapidus)in Chesapeake Bay[C].//Hines A H.First Europan Crustacean Conference.Paris:1992:67-68.
[4]Minagawa M.Influence of temperature on survival,feeding and de-velopment of larvae of the red frog crab,Ranina ranina(Crustacea,Decapoda,Raninidae)[J].Jap soc sci fish,1990,56(5):755-760.
[5]李太武.三疣梭子蟹精子的发生及超微结构的研究[J].动物学报,1995,41(1):41-47.
[6]Pile AJ,R N Lipcius,J V Montfrans,et al.Density-dependent set-tler recruit juvenile relationships in blue crabs[J].Ecological Mono-graphs,1996,66(3):277-300.
[7]胡毅,潘鲁青.三疣梭子蟹消化酶的初步研究[J].中国海洋大学学报:自然科学版,2006,36(4):621-626.
[8]Richard R A.Habitat selection and predator avoidance:Ontogeneticshifts in habitatuse bythe JonahcrabCancer borealis(Sti mpson)[J].J Exp Map Biol Ecol,1992,156(2):187-197.
[9]朱冬发,王春琳,李志强,等.三疣梭子蟹核型分析[J].水产学报,2005,29(5):649-653.
[10]Allen R Place,Feng XJ,Colin R Steven.Genetic markers in bluecrabsⅡComplete Mitochondrial genome sequence and characteriza-tion of genetic variation[J].Experi mental Marine Biology andEcolocgy,2005,319:15-27.
[11]Mitsugu M,Yamauchi.Complete mitochondrial DNAsequence ofswi mming crabPortunus triuberbuculatus(Crustacea:Decapoda:Brachyura)[J].Gene,2003,311:129-135.
[12]薛俊增,堵南山,赖伟.中国三疣梭子蟹Portunus triuberbucu-latus Miers的研究[J].东南海洋,1997,15(1):60-65.
[13]戴爱云,杨思琼,宋玉枝,等.中国海洋蟹类[M].北京:海洋出版社,1986.
[14]Kobayashi S,Matsuura S.Variation of the duration of copulation ofthe Japanese mitten crabEriocheirjaponicus[J].Journal of Etholo-gy,1994,12:73-76.
[15]高保全,刘萍,李健,等.三疣梭子蟹野生群体同功酶的遗传多样性分析[J].水产学报,2007,31(1):1-6.
[16]徐敬明.蟹类线粒体DNA的研究与应用[J].中国海洋大学学报:自然科学版,2006,36(6):879-884.
[17]Zane L,Bargelloni L,Patarnello T.Strategies for microsatellitesisolation:a review[J].Mol Ecol,2002,11:1-16.
[18]Pamela C Jensen,Paul Bentzen.Isolation and inheritance of mi-crosatelliteloci inthe Dungeness crab(Brachyura:Cancridae:Cancermagister)[J].Genome,2004,47:325-331.
[19]Masatsugu Takano,Anna Barinova,Takuma Sugaya,et al.Isolationand characterization of microsatellite DNA markers from mangrovecrab,Scylla paramamosain[J].Molecular Ecology Notes,2005,5:794-795.
[20]David Gopurenko,Jane M.Hughes,Jing Ma.Identification ofpolymorphic microsatellite loci in the mud crabScylla serrata(Brachyura:Portunidae)[J].Molecular Ecology Notes,2002,2:481-483.
[21]E S Yap,E Sezmis,J A Chaplin.Isolation and characterization ofmicrosatellite loci inPortunus pelagicus(Crustacea:Portunidae)[J].Molecular Ecology Notes,2002,2(1):30-32.
[22]Puebla O,Parent,J MSévigny.New microsatellite markers forthe snowcrabChionoecetes opilio(Brachyura:Majidae)[J].Molec-ular Ecology Notes,2003,3(4):644-646.
[23]An HS,Jeong J H,Park J Y.New microsatellite markers for thesnowcrabChionoecetes opilio(Brachyura:Majidae)[J].MolecularEcology Notes,2006,7(1):86-88.
[24]Hanfling B,Weetman D.Characterization of microsatellite loci forthe Chinese mitten crab,Eriocheir sinensis[J].Molecular EcologyNotes,2003,3(1):15-17.
[25]Chang Y M,Liang L Q,Li S W,et al.Aset of new microsatelliteloci isolated from Chinese mitten crab,Eriocheir sinensis[J].Molecular Ecology Notes,2006,6(4):1237-1239.
[26]胡维,向华,周艳,等.用PCR法直接快速筛查重组阳性克隆[J].生物技术通报,1999,15:39-43.
[27]Colin R Steven T,Jessica Hill,Brian Masters,et al.Genetic mark-ers in blue crabs(Callinectes sapidus)I:Isolation and characteriza-tion of microsatellite markers[J].Journal of Experi mental MarineBiology and Ecology,2005,319:3-14.
[28]生秀杰,周伟强,姜莉,等.应用以菌落为模板的聚合酶链反应技术筛选重组阳性克隆[J].中华检验医学杂志,2002,25(4):239-240.
[29]朱泽远,王亚菊,施田辉,等.荧光标记微卫星分析人工饲养中华绒螯蟹的遗传多样性[J].中国海洋大学学报:自然科学版,2007,27(4):591-596.
[30]Weber J L.Informativeness of human(dC-dA)n(dG-dT)n poly-morphisms[J].Genomics,1990,7:524-530.
[31]Tam,Y K,Kornfield.Characterization of microsatellite markers inHomarus(Crustacea,Decapoda)[J].Mol Mar Biol Biotechnol,1996,5:230-238.
[32]Sugaya T,Ikeda M,Mori H,et al.Inheritance mode of microsatel-lite DNA markers and their use for kinship esti mation in kurumaprawnPenaeus japonicus[J].Fish Sci,2002,68:299-305.
[33]Belfiore,N M,May B.Variable microsatellite loci in red swampcrayfish,Procambarus clarkii,and their characterization in othercrayfish taxa[J].MolEcol,2000,9:2230-2234.
[34]Bentzen P,Taggart C T,Ruzzante,et al.Microsatellite polymor-phismand the population structure of Atlantic cod(Gadus morhua)in the northwest Atlantic[J].Can J Fish Aquat Sci,1996,53:2706-2721.
[35]Allen PJ,Amos W,Pomeroy,et al.Microsatellite variationin greyseals(Halichoerus grypus)shows evidence of genetic differentiationbetweentwo British breedingcolonies[J].Mol Ecol,1995,4:653-662.
[36]Baker N,Byrne K,Moore S,et al.Characterization of microsatel-liteloci in the redclawcrayfish,Cherax quadricarinatus[J].MolEcol,2000,9:494-495.
[37]Callen D F,Thompson A D,Shen Y,et al.Incidence and origin of“null”alleles in the(AC)n microsatellite markers[J].AmJ HumGenet,1993,52:922-927.
[38]Pemberton J M,Slate J,Bancroft D R,et al.Nonamplifying allelesat microsatellite loci:Acaution for parentage and population studies[J].Molecular Ecology,1995,4:249-252.
[39]Vigouroux Y,Jaqueth J S,Matsuoka Y,et al.Rate and pattern ofmutation at microsatellite loci in maize[J].Mol Biol Evol,2002,19:1251-1260.
[40]Pemberton J M,Slate J,Bancroft D R,et al.Nonamplifying allelesat microsatellite loci:Acaution for parentage and population studies[J].Mol Ecol,1995,4:249-252.
[41]Jones A G,Stockwell C A,Walker D,et al.The molecular basis ofa microsatellite null allele from the white sands pupfish[J].JHered,1998,89:339-342.
[42]Sugaya T,Ikeda M,Mori H,et al.Inheritance mode of microsatel-lite DNA markers and their use for kinship esti mation in kurumaprawnPenaeus japonicus[J].Fish Sci,2002,68:299-305.
[43]Murray V,Monchawin C,England P R.The determination of thesequences present in the shadow bands of a dinucleotide repeat PCR[J].Nucleic Acids Res,1993,21:2395-2398.
[44]Hauge X Y,Litt M.Astudy of the origin of shadow bands’seenwhen typing dinucleotide repeat polymorphisms by the PCR[J].Hum Mol Genet,1993,2:411-415.
[45]Shinde D,Lai Y,Sun F,et al.Taq DNApolymerase slippage muta-tion rates measured by PCRand quasi-likelihood analysis:(CA/GT)n and(A/T)n microsatellites[J].Nucleic Acids Res,2003,31:974-980.
[46]Tully G,Sullivan K M,Gill P.Analysis of 6 VNTRloci by’multi-plex’PCR and automated fluorescent detection[J].Hum Genet1993,92:554-562.
[47]Reilly P T,Herbinger C,Wright J M.Analysis of parentage deter-minationin Atlantic sal mon using microsatellites[J].Ani m Genet,1998,29:363-370.
[48]马海涛,常玉梅,于冬梅,等.利用微卫星分子标记分析四个中华绒蟹群体的遗传多样性[J].动物学研究,2007,28(2):126-133.
基本信息:
DOI：10.16441/j.cnki.hdxb.2008.05.004
中图分类号:S917.4
引用信息:
[1]刘萍,宋来鹏,李健,等.蟹类微卫星DNA标记的筛选及其在遗传学研究中的应用[J].中国海洋大学学报(自然科学版),2008,No.164(05):712-718.DOI:10.16441/j.cnki.hdxb.2008.05.004.
基金信息:
国家高技术研究发展计划项目(2006AA10A406);; 科技部平台项目(2004DKA30470);; 青岛市科技计划项目(07-2-3-5-Jch)资助
2008-09-15
2008-09-15