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研究以中国刺参和日本红刺参为材料,进行了4个组合的杂交和自交实验,分析了各实验组受精率、孵化率、幼虫和稚参阶段的生长以及杂交子代的杂种优势。结果表明,杂交组的受精率、孵化率均低于自交组,但是经单因素方差分析表明没有显著性差异(P>0.05);日本红刺参♀×中国刺参♂杂交组(JC)在幼虫期和稚参期的各生长性状平均值均大于其它3组,并表现出显著性差异;日本红刺参♀×中国刺参♂组(JC)的体长和体重的杂种优势率均较为明显,其幼虫体长和稚参体长、体重的杂种优势率分别在4.60%6.90%,8.39%~18.85%和23.21%~63.74%之间;中国刺参♀×日本红刺参♂组(CJ)的杂种优势率在160日龄后表现为正值。杂交子一代中存在正交和反交结果不同的现象可能与母性效应、性别连锁和细胞质遗传有关。
Abstract：Intraspecific hybridization was investigated using two stocks(C and J) of the sea cucumber(Stichopus japonicus).Stock C was sampled from Lidao,Shandong province,China.Stock J was derived from a red type of S.japonicus which was introduced from Oita,Japan.The fertilization rate,larval survival rate,growth and heterosis at larval and juvenile stages were compared between two reciprocal hybrid crosses (C♀×J♂,CJ and J♀ × C♂,JC) and two parental groups(C♀×C♂, CC and J♀×J♂,JJ).The results indicated that the rates of fertilization and larval survival of hybrid crosses were inferior to those of parental groups,but did not show statistically marked significant difference(P>0.05).The growth performance at larval and juvenile stages of JC cross was superferior to the other three groups,showing marked heterosis in the characteristics of body length and weight growth.The heterosis of the JC cross ranged from 4.60% to 6.90% for larval body length, from 8.39% to 18.85% for juvenile body length,and from 23.21% to 49.77% for juvenile body weight.In contrast,the CJ cross did not show positive heterosis until 160 days had passed after fertilization.The difference in heterosis between the two reciprocal hybrid crosses might be related to the maternal effects,sex linkage and cytoplasmic inheritance.
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基本信息:
DOI：10.16441/j.cnki.hdxb.2009.s1.068
中图分类号:S917.4
引用信息:
[1]胡美燕,李琪,孔令锋,等.中国刺参与日本红刺参杂交子一代的早期生长比较[J].中国海洋大学学报(自然科学版),2009,39(S1):375-380.DOI:10.16441/j.cnki.hdxb.2009.s1.068.
基金信息:
国家科技支撑计划项目(2006BAD09A01);; 国家高技术研究发展计划项目(2006AA10A409)资助
2009-08-15
2009-08-15