| 606 | 33 | 29 |
| 下载次数 | 被引频次 | 阅读次数 |
自然发病的养殖大菱鲆幼苗出现口部周围皮下出血,肝脏灰白色并萎缩坏死的症状。从肝脏中分离纯化得到优势菌株,定名为db14。该菌株在LB培养基上生长较缓慢,早期菌落稍隆起,白色,质地较硬;人工感染试验证实对大菱鲆有较强的致病性,其半数致死剂量LD50为2.75×103cfu/g;生理生化特征测定显示其与杀鲑气单胞菌无色亚种(Aeromonas salmonicidasubsp.achromogenes)相似度最高;细菌16S rDNA序列测定后在GenBank中进行序列比对分析,结果与杀鲑气单胞菌无色亚种的序列同源性最高,为100%。综合上述结果,确定该菌株为杀鲑气单胞菌无色亚种。该菌株对复达欣、菌必治、丁胺卡那霉素和氯霉素等较为敏感。通过对人工感染菌株db14的大菱鲆组织切片观察,发现病鱼的肝脏、肾脏、脾脏、肠道等器官的组织均有明显的病理变化,而心脏和脑未发现明显异常。
Abstract：A strain of bacterial pathogen named as db14 was isolated from a cultured turbot(Scophthalmus maximus) fry with the symptom of subcutaneous hemorrhage around the mouth,and gray and atrophyic liver.Strain db14 grew slowly on the LB medium,and the colonies were caelate,white and hard. The intraperitoneal injection experiment revealed that the strain was virulent to turbot with an LD50 value of 2.75×103 colony forming units/g.The strain was similar in biochemical and biophysical properties to Aeromonas salmonicida subsp.achromogenes.Compared to the similar sequences in the GenBank database,the sequence of 16S rDNA of strain db14 shared a high similarity to that of A.salmonicida subsp.achromogenes.Based on the results obtained,strain db14 was identified as A.salmonicida subsp.achromogenes.The chemotherapeutant sensitivity test showed that fortum,chloramphenicol,ceftriaxone and amikacin could effectively inhibit the growth of the strain db14.When challenged by strain db14,there were obvious pathological changes in the turbot tissue of liver,kidney,spleen and intestine in the pathological sections of the fish,and the tissues of the heart and brain looked normal.
[1]邹玉霞,张培军,莫照兰,等.大菱鲆出血症病原菌的分离和鉴定[J].高技术通讯,2004,14(4):89-93.
[2]陈吉祥,李彩风,颜显辉,等.大菱鲆病原鳗弧菌生物学及分子特征研究[J].高技术通讯,2005,15(6):92-96.
[3]范文辉,黄,王秀华,等.养殖大菱鲆溃疡症病原菌的分离鉴定及系统发育分析[J].微生物学报,2005,45(5):665-670.
[4]张伟妮,周丽,邢婧,等.养殖大菱鲆腹水症病原菌SR1的分离及鉴定[J].中国水产科学,2006,13(4):603-609.
[5]姚志刚,丁天宝.大菱鲆育苗期的细菌病研究[J].海洋科学,2004,28(9):10-12.
[6]李筠,颜显辉,陈吉祥,等.养殖大菱鲆腹水病病原的研究[J].中国海洋大学学报:自然科学版,2006,36(4):649-654.
[7]Wardlaw,A C.Practical statistics for experimental biologists[M].John Wiley and Sons,Chichester,UK,1985.
[8]沈萍,范秀容,李广武.微生物学实验[M].(第三版).北京:高等教育出版社,2004:28-37.
[9]Spangler B D.Methods in molecular biology and protein chemistry[M].New York:John Wiley&Sons,Ltd,2002.
[10]Holt J G,Krieg N R,Sneath P H A,et al.Bergey’s manual of de-terminative bacteriology[M].Ninth Edition.1994:251-253.
[11]曲芬.气单胞菌感染的研究进展[J].传染病信息2006,19(1):22-23.
[12]Pavan M E,Abbott S L,Zorzopulos J,et al.Aeromonas salmonici-dasubsp.pectinolyticasubsp.nov.,a new pectinase-positive sub-species isolated from a heavily polluted river[J].International Jour-nal of Systematic and Evolutionary Microbiology,2000,50:1119-1124.
[13]Nash J H E,Findlay W A,Luebbert C C,et al.Comparative ge-nomics profiling of clinical isolates ofAeromonas salmonicidausingDNA microarrays[J].BMC Genomics,2006,7:43.
[14]Austin B,Austin D A.Bacterial fish pathogens:disease in farmedand wild fish[M].Chichester,Ellis Horwood Limited,1987.
[15]丁雷,岳永生,宋憬愚.虹鳟皮肤溃烂病的病原菌研究[J].淡水渔业,2002,32(3):28-30.
[16]李健,陈沁,王巧全,等.出口渔场杀鲑气单胞菌杀鲑亚种的分离鉴定[J].黑龙江畜牧兽医,2003,4:38-39.
[17]张晓君,房海,陈翠珍,等.杀鲑气单胞菌一新亚种的生物学特性及系统发育学分析[J].中国水产科学,2006,13(60):917-922.
[18]Nougayreda P,Sochon E,Vuillaume A.Isolation ofAeromonassalmonicidasubspeciessalmonicidain farmed turbot(Psetta maxi-ma)in France[J].Bull Eur Ass Fish Pathol,1990,10:139-140.
[19]Toranzo A E,Santos Y,Nuriez S,et al.Biochemical and serologicalcharacteristics drug resistance and plasmid profiles of Spanish isolatesofAeromonas salmonicida[J].Fish Pathol,1991,26:55-60.
[20]Bjornsdottir B,Gudmundsdottir S,Bambir S H,et al.Experimentalinfection of turbot,Scophthalmus maximus(L.),byAeromonassalmonicidasubsp.achromogenesand evaluation of cross protectioninduced by a furunculosis vaccine[J].Journal of Fish Diseases,28(3):181-188.
[21]Wang Z,Liu X,Dacanay A,et al.Carbohydrate analysis and sero-logical classification of typical and atypical isolates ofAeromonassalmonicida:a rationale for the lipopolysaccharide-based classifica-tion ofA.salmonicida[J].Fish&Shellfish Immunology,2007,23(5):1095-1106.
[22]Bjornsdottir B,Gudmundsdottir S,Bambir S H,et al.Induction ofinflammatory cytokines by extracellular products and LPS of the fishpathogenAeromonas salmonicidassp.achromogenesin mice andmouse cell cultures[J].Veterinary Immunology and Im-munopathology,2001,81(1-2):71-83.
[23]Mulder I E,Wadsworth S,Secombes C J.Cytokine expression inthe intestine of rainbow trout(Oncorhynchus mykiss)during infec-tion withAeromonas salmonicida[J].Fish&Shellfish Immunolo-gy,2007,23:747-759.
[24]Lin B,Chen S,Cao Z,et al.Acute phase response in zebrafish uponAeromonas salmonicidaandStaphylococcus aureusinfection:Strik-ing similarities and obvious differences with mammals[J].MolecularImmunology,2007,44:295-301.
[25]Neji H,Noue J.Effect of animal and vegetal protein diets on feed in-take and apparent digestibility of nutrients in rainbow trout(On-corhynchus mykiss)infected byAeromonas salmonicida,with andwithout chronic hypoxia stress[J].Canadian Journal of Fisheriesand Aquatic,1998,55(9):2019-2027.
基本信息:
DOI：10.16441/j.cnki.hdxb.2009.01.016
中图分类号:S943
引用信息:
[1]吕俊超,张晓华,王燕,等.养殖大菱鲆病原菌——杀鲑气单胞菌无色亚种的分离鉴定和组织病理学研究[J].中国海洋大学学报(自然科学版),2009,39(01):91-95.DOI:10.16441/j.cnki.hdxb.2009.01.016.
基金信息:
国家高技术研究发展计划项目(2007AA09Z434);; 教育部新世纪优秀人才支持计划项目(NCET-04-0645)资助
2009-01-15
2009-01-15