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本文以金刚虾(Penaeus monodon)为研究对象,采用生物絮团技术(Biofloc Techonology,BFT),在室内水泥池进行为期30d的监测,分析生物絮团对于水质、金刚虾生长和非特异性免疫酶活性的影响。实验分为实验组和对照组,实验组以红糖作为碳源,定期向水体中泼洒金贝斯2号微生态制剂,对照组采用普通的养殖法,1~15d根据养殖水质少量换水,15d后每天换水60%。实验第7天透可见水中微小悬浮物,生物絮团生成。实验第15、30天抽取血样,检测血清中酚氧化酶(PO)、酸性磷酸酶(ACP)、超氧化物歧化酶(SOD)溶菌酶(LZM)的活性,并称量试验结束时金刚虾的体重、体长和养殖结束时的体长、体重和总产量。试验结果表明:实验组金刚虾生物学体长以及体重远超对照组,差异显著(P<0.05);而实验组PO、ACP、SOD、LZM活性明显高于对照组(P<0.05),并且PO、ACP、LZM在实验期间变化趋势相似,实验第15天的数值要大于实验第30天;SOD是实验第30天大于第15天;实验结果证明了生物絮团技术对养殖水体中的氨氮和亚硝酸氮有很好的去除作用(P<0.05)。养殖结束后,实验组产量为143.5kg/池明显的高于对照组106kg/池,实验组的成活率64.6%显著的高于对照组的成活率56.8%(P<0.05)。因此,以生物絮团技术养殖金刚虾不但能够促进金刚虾的生长,还能够提高金刚虾的免疫力,净化水质,这为实际生产提供理论依据。
Abstract：We evaluated the effects of bioflocs on growth and immunity of Penaeus monodon during a 30 d feeding trial.The experiment is divided into the experimental group and control group.The experimental group was treated with sugar as carbon source,to pour probiotics into water regularly and control group without any treatment.The blood was sampled on 15 days and 30 days to measure the activities of PO,ACP,SOD and LZM.The results showed that the biological body length and weight were higher than the control group significantly(P<0.05)and the activities of PO,ACP,SOD and LZM were also higher than control group significantly(P<0.05).The dates on the 15 th day were significantly higher than that on the 30 th day,but the date of SOD was on the contrary,indicating that the effect of bioflocs on activities of SOD had a longer time.The result had also shown that bioflocs had brought down the ammonia nitrogen and nitrite nitrogen significantly.At the end,the yield of the experimental group was 143.5 kg/pool,which was significantly higher than the control group of 106 kg/pool.The survival rate of the experimental group was 64.6%significantly higher than that of the control group,which was 56.8%(P<0.05).In a result,the Biofloc Techonology,BFT not only could promote the growth of Penaeus monodon,but can enhance the immunity of Penaeus monodon.
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基本信息:
DOI：10.16441/j.cnki.hdxb.20180114
中图分类号:S968.22
引用信息:
[1]叶海斌,王友红,王晓璐,等.生物絮团对金刚虾的生长和非特异性免疫酶活性的实验研究[J].中国海洋大学学报(自然科学版),2018,48(S2):52-58.DOI:10.16441/j.cnki.hdxb.20180114.
基金信息:
山东省现代农业产业技术体系虾蟹类创新团队项目(SDAIT-13)资助~~
2018-12-10
2018-12-10