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温度会对鱼类的营养需求产生影响，为研究不同温度条件下不同饲料方案对大菱鲆(Scophthalmus maximus L.)幼鱼生长和氧化应激的影响，确定不同温度下最适合的饲料方案。设计了等脂等能的4种实验饲料，分别为45%蛋白15%糊精的低蛋白组(LP组)、50%蛋白10%糊精的对照组(CON组)、55%蛋白0%糊精的高蛋白组(HP组)和50%蛋白20%糊精的高糖组(HC组)。在3个温度条件下(15、18和21℃)挑选初始质量(7.50±0.10) g的幼鱼进行8周的养殖实验，并对生长、抗氧化应激指标和抗氧化指标应激相关基因表达情况进行研究。研究显示，15℃时，CON组和LP组的特定生长率(SGR)显著高于HP组(P<0.05),且CON组和LP组肝脏丙二醛(MDA)含量显著低于HP组和HC组(P<0.05)。18℃时，CON组的SGR最高，显著高于LP组(P<0.05);且CON组肝脏MDA含量最低(P<0.05)。21℃时，HP组有最高的SGR和最低的饲料系数(FC)(P<0.05),且HP组肝脏MDA含量显著低于LP组和HC组(P<0.05)。在本实验3种温度条件下HC组肝脏MDA含量都最高；同时，CON组和HP组可通过上调Nrf2-ARE信号通路中相关基因来提高鱼体抗氧化能力。研究结果表明，在低温度下适合投喂低中蛋白饲料，在适温度条件下适合投喂中蛋白饲料，在高温度条件下适合投喂高蛋白饲料，高糖饲料会导致严重的氧化应激。
Abstract：Temperature affects the nutrient requirement of fish. In order to study the optimal diet for juvenile turbot(Scophthalmus maximus L.) under different temperature conditions and the effects of different diet strategies on the growth and oxidative stress of turbot, four experimental diets with isolipid and isoergy were designed and compared. The compared included the low protein group(LP group) with 45% protein and 15% dextrin, control group(CON group) with 50% protein and 10% dextrin, high protein group(HP group) with 50% protein and 10% dextrin and high sugar group(HC group) with 50% protein and 20% dextrin. At three temperatures(15, 18 and 21 ℃), juvenile fish with an initial mass of(7.50±0.10) g were selected and cultured for 8 weeks. The results showed that the specific growth rate(SGR) of the CON group and the LP group was significantly higher than that of HP group(P<0.05) at 15 ℃, and the liver malondialdehyde(MDA) content of the CON group and the LP group was significantly lower than that of HP group and HC group(P<0.05). The CON group had the highest SGR, significantly higher than of LP group(P<0.05) at 18 ℃, and the CON group had the lowest MDA content in the liver(P<0.05). The HP group had the highest SGR and the lowest feed coefficient(FC)(P<0.05) at 21 ℃, and the liver MDA content was significantly lower than that of the LP and HC groups(P<0.05). In addition, the liver MDA content of the HC group was the highest at three temperatures, and the CON group and the HP group improved the antioxidant capacity of fish by up-regulating the expression of genes functioning in Nrf2-ARE signaling pathway. Our findings demonstrated that low and medium protein diets are suitable for feeding at low temperatures; medium protein diets are suitable for feeding at moderate temperatures; high protein diets are suitable for feeding at high temperatures; and high sugar diets cause severe oxidative stressing.
[1] Radhakrishnan G,Shivkumar,Mannur V S,et al.Dietary protein requirement for maintenance,growth,and reproduction in fish:A review[J].Journal of Entomology and Zoology Studies,2020,8(4):208-215.
[2] Gauquelin F,Cuzon G,Gaxiola G,et al.Effect of dietary protein level on growth and energy utilization by Litopenaeus stylirostris under laboratory conditions[J].Aquaculture,2007,271(1-4):439-448.
[3] Wilson R P.Utilization of dietary carbohydrate by fish[J].Aquaculture,1994,124(1-4):67-80.
[4] Fernandez F,Miquel A G,Cordoba M,et al.Effects of diets with distinct protein-to-carbohydrate ratios on nutrient digestibility,growth performance,body composition and liver intermediary enzyme activities in gilthead sea bream (Sparus aurata,L.) fingerlings[J].Journal of Experimental Marine Biology & Ecology,2007,343(1):1-10.
[5] 陈俊行.饲料中糖和脂肪水平对吉富罗非鱼生长、体组成、外周组织糖代谢和糖耐受的影响[D].重庆：西南大学，2021.Chen J H.Effects of Dietary Carbohydrate and Lipid Levels on the Growth,Body Composition,Peripheral Glucose Metabolism and Glucose Tolerance of Genetically Improved Farmed Tilapia Oreochromis niloticus[D].Chongqing:Southwest University,2021.
[6] Wassana P,Panita P,Ivan J,et al.Transcriptomics,metabolomics and histology indicate that high-carbohydrate diet negatively affects the liver health of blunt snout bream (Megalobrama amblycephala)[J].BMC Genomics,2017,18(1):856.
[7] Nellore J,Cynthia P P,Mohanan S P,et al.Vinca rosea normalizes oxidative stress and inhibits hyperglycemia induced increase in VEGF in zebrafish retina[J].Research Journal of Pharmaceutical,Biological and Chemical Sciences,2013,4(4):927-936.
[8] Wu C,Chen L,Lu Z,et al.The effects of dietary carbohydrate on the growth,antioxidant capacities,innate immune responses and pathogen resistance of juvenile Black carp Mylopharyngodon piceus[J].Fish & Shellfish Immunology,2016,49:132-142.
[9] An M I,Choi C Y.Activity of antioxidant enzymes and physiological responses in ark shell,Scapharca broughtonii,exposed to thermal and osmotic stress:Effects on hemolymph and biochemical parameters[J].Comparative Biochemistry & Physiology Part B Biochemistry & Molecular Biology,2010,155(1):34-42.
[10] Tort L,Rotllant J,Rovira L.Immunological suppression in gilthead sea bream Sparus aurata of the North-West Mediterranean at low temperatures[J].Comparative Biochemistry & Physiology A,1998,120(1):175-179.
[11] Bl A,Bo L,Qz B,et al.Effects of different temperatures and protein levels on growth performance,physiological response and expression of immune-related genes of juvenile oriental river prawn (Macrobrachium nipponense)[J].Aquaculture,2021,536:736435.
[12] Qiang J,Yang H,Wang H,et al.Growth and IGF-I response of juvenile Nile tilapia (Oreochromis niloticus) to changes in water temperature and dietary protein level[J].Journal of Thermal Biology,2012,37(8):686-695.
[13] Ruyet P L.Turbot (Scophthalmus maximus) grow-out in Europe:practices,results,and prospects[J].Turkish Journal of Fisheries & Aquatic Sciences,2002,2:29-39.
[14] Aydin L,Ercan K,Polat H,et al.Growth and feed conversion ratio of diploid and triploid induced juvenile turbot reared at different water temperatures[J].Aquaculture,2021,543:736981.
[15] 陈四清，马爱军，雷霁霖，等.大菱鲆幼鱼的蛋白质与能量需求[J].水产学报，2004,28(4):425-430.Chen S Q,Ma A J,Lei J L,et al.Requirement of protein and energy for juvenile Scophthalmus maximus[J] Journal of Fisheries of China,2004,28(4):425-430.
[16] Lee J K,Cho S H,Park S U,et al.Dietary protein requirement for young turbot (Scophthalmus maximus L.)[J].Aquaculture Nutrition,2003,9(4):283-286.
[17] Li Y,Jiang K Y,SUN G X,et al.The eco-nutrition requirements for dietary protein and its rhomb characteristics in juvenile turbot (Scophthalmus maximus L.)[J].Journal of Oceanology and Limnology,2011,29(5):1002-1008.
[18] Liu X,Mai K,Liufu Z,et al.Effects of dietary protein and lipid levels on growth,nutrient utilization,and the whole-body composition of turbot,Scophthalmus maximus,Linnaeus 1758,at different growth stages[J].Journal of the World Aquaculture Society,2014,45(4):355-366.
[19] 李晓宁.饲料糖水平对大菱鲆和牙鲆生长、生理状态参数及体组成的影响[D].青岛：中国海洋大学，2011.Li X N.Effects of Dietary Carbohydrate Levels on Growth Performance,Physiological Status and Body Composition of Turbot (Scophthalmus maximus Linnaeus) and Japanese Flounder (Paralichthys olivaceus)[D].Qingdao:Ocean University of China,2011.
[20] Liu D,Zhang Y,Pan M,et al.Interactive effects of dietary biotin and carbohydrate on growth performance and glucose metabolism in juvenile turbot Scophthalmus maximus L[J].Aquaculture,2021(3):736752.
[21] 付丰顺，刘成栋，王旋，等.大菱鲆对饲料精氨酸的需求量及饲料精氨酸水平对大菱鲆生长和代谢的影响[J].水产学报，2021,45(10):1692-1702.Fu F S,Liu C D,Wang X,et al.Dietary arginine requirement and effects of dietary arginine levels on the metabolism of Scophthalmus maximus[J].Journal of Fisheries of China,2021,45(10):1692-1702.
[22] Horwitz W,Latimer G W.Official Methods of Analysis of AOAC International[M].18th Edition.USA:Association of Official Analytical Chemists,2005.
[23] Rawles S D,Green B W,Gaylord T G,et al.Response of sunshine bass (Morone chrysops×M.saxatilis) to digestible protein/dietary lipid density and ration size at summer culture temperatures in the Southern United States[J].Aquaculture,2012,356:80-90.
[24] Cai L S,Wang L,Song K,et al.Evaluation of protein requirement of spotted seabass (Lateolabrax maculatus) under two temperatures,and the liver transcriptome response to thermal stress[J].Aquaculture,2020,516:734615.
[25] Alexander C,Sahu N P,Pal A K,et al.Higher water temperature enhances dietary carbohydrate utilization and growth performance in Labeo rohita (Hamilton) fingerlings[J].Journal of Animal Physiology & Animal Nutrition,2011,95(5):642-652.
[26] 黄金凤.不同温度下松浦镜鲤幼鱼蛋白质需求量的研究[D].上海：上海海洋大学，2013.Huang J F.Study on the Protein Requirement of Juvenile Mirror Carp (Cyprinus carpio) at Different Temperature[D].Shanghai:Shanghai Ocean University,2013.
[27] Liang H,Xu H,Ge X P,et al.Water temperature affects the protein requirements,growth performance,and nutritional metabolism of grass carp (Ctenopharyngodon idella) juveniles[J].Aquaculture Reports,2022,25:101267.
[28] Derubertis F R,Cravens P A.Oxidative and glycooxidative stress in diabetic nephropathy[J].The Diabetic Kidney,2006(1):151-172.DOI:doi.org/10.1007/978-1-59745-153-6_9.
[29] Forman H J,Davies K,Ursini F.How do nutritional antioxidants really work:Nucleophilic tone and para-hormesis versus free radical scavenging in vivo[J].Free Radical Biology & Medicine,2014,66:24-35.
[30] 马骏，李勇，张静，等.3种非营养性抗氧化剂在水产动物中的研究进展[J].水产科学，2018,37(3):414-420.Ma J,Li Y,Zhang J,et al.Research progress on three non-nutritive antioxidantsin aquatic animals:A review[J].Fisheries Science,2018,37(3):414-420.
[31] Mozanzadeh M T,Marammazi J G,Yavari V,et al.Dietary n-3 LC-PUFA requirements in silvery-black porgy juveniles (Sparidentex hasta)[J].Aquaculture,2015,448:151-161.
[32] Xu C,Liu W B,Shi H J,et al.Benfotiamine ameliorates high-carbohydrate diet-induced hepatic oxidative stress,inflammation and apoptosis in Megalobrama amblycephala[J].Aquaculture Research,2021,52(7):3174-3185.
[33] Gao J,Koshio S,Ishikawa M,et al.Effect of dietary oxidized fish oil and vitamin C supplementation on growth performance and reduction of oxidative stress in Red Sea Bream Pagrus major[J].Aquaculture Nutrition,2013,19(1):35-44.
[34] Efrati S,Bechor Y,Hadanny A,et al.Seizures during hyperbaric oxygen therapy:Retrospective analysis of 62 614 treatment sessions[J].Undersea and Hyperbaric Medicine:Journal of the Undersea and Hyperbaric Medical Society,2016,43(1):21-28.
[35] Ostrowski R,Stepien K,Pucko E,et al.Hyperbaric oxygen modalities are differentially effective in distinct brain ischemia models[J].Medical Gas Research,2016,6(1):39-47.
[36] 范鹏涛，杨菲，刘保成，等.高压氧对模型大鼠颅脑损伤CAT、SOD、GSH-Px和Nrf2影响的研究[J].神经解剖学杂志，2017,33(3):280-284.Fan P T,Yang F,Liu B C,et al.Effects of hyperbaric oxygen on CAT,SOD,GSH-Px and Nrf2 in model rats with brain injury[J].Chinese Journal of Neuroanatomy,2017,33(3):280-284.
[37] 胡流芳，王迎，任汝静，等.Keap1-Nrf2/ARE信号通路的抗氧化应激作用及其调控机制[J].国际药学研究杂志，2016,43(1):146-152.Hu L F,Wang Y,Ren R J,et al.Anti-oxidative stress actions and regulation mechanisms of Keap1-Nrf2/ARE signal pathway[J].Journal of International Pharmaceutical Research,2016,43(1):146-152.
[38] Wen M L,Feng L,Jiang W D,et al.Thiamin deficiency induces impaired fish gill immune responses,tight junction protein expression and antioxidant capacity:Roles of the NF-kappa B,TOR,p38 MAPK and Nrf2 signaling molecules[J].Fish & Shellfish Immunology,2016,51:373-383.
[39] Jiang W D,Feng L,Qu B,et al.Changes in integrity of the gill during histidine deficiency or excess due to depression of cellular anti-oxidative ability,induction of apoptosis,inflammation and impair of cell-cell tight junctions related to Nrf2,TOR and NF-κB signaling in fish[J].Fish & Shellfish Immunology,2016,56:111-122.
[40] Pan F Y,Feng L,Jiang W D,et al.Methionine hydroxy analogue enhanced fish immunity via modulation of NF-κB,TOR,MLCK,MAPKs and Nrf2 signaling in young grass carp (Ctenopharyngodon idella)[J].Fish & Shellfish Immunology,2016,56:208-228.
[41] 姚琦，郑男，郭志欣，等.水生生物Nrf2-Keap1/Are信号通路的研究进展[J].中国畜牧兽医，2021,48(2):492-500.DOI:10.16431/j.cnki.1671-7236.2021.02.011.Yao Q,Zheng N,Guo Z X,et al.Advances in the Nrf2-Keapl/Are signaling pathway in aquatic organism[J].China Animal Husbandry & Veterinary Medicine,2021,48(2):492-500.DOI:10.16431/j.cnki.1671-7236.2021.02.011.
基本信息:
DOI：10.16441/j.cnki.hdxb.20230135
中图分类号:S965.399
引用信息:
[1]吴唐飞,刘成栋,王旋,等.三种温度下不同饲料方案对大菱鲆幼鱼生长和氧化应激的影响[J].中国海洋大学学报(自然科学版),2024,54(07):130-138.DOI:10.16441/j.cnki.hdxb.20230135.
基金信息:
国家重点研究发展计划项目(2018YFD0900400); 国家现代农业产业技术体系项目(CARS-47-G10)资助~~
2023-04-07
2023
2023-05-15
2023
2023-05-09
1
2024-06-19
2024-06-19