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采用AFLP技术对中国对虾野生群体和第9、10代选育群体的遗传多样性及遗传分化进行分析,计算了3个群体间的遗传多态度、遗传距离及分化系数。结果显示,5对引物共产生了137条带,其中多态性条带有63条,平均每对引物检测到12.6条多态性条带。3个群体的多态位点比例分别为45.99%、40.57%和41.02%;Shannon多样性指数分别为0.1818,0.1807和0.1774;野生群体与选育群体之间的遗传距离及分化都比较大,但选育群体之间的遗传距离和分化都很小分别为0.0031和0.0206。结果表明,选育群体相较于野生群体多态位点比例和遗传多态度均有所下降,随着选育时间的延长,相邻世代群体间遗传距离和分化也均有所下降,出现趋同现象,显示出"黄海1号"新品种具有遗传上的稳定性。
Abstract：The AFLP technique was used to analyze the genetic diversity and genetic differentiation of a wild population and 2 consecutive selected populations of shrimp Fenneropenaeus Chinensis.The genetic polymorphisms,genetic distances and differentiation indexes were calculated according to the AFLP results.The results showed that 137 bands were generated with 5 primer sets,and 63 bands were polymorphic bands.On the average,every primer set had detected 12.6 polymorphic bands.The proportions of polymorphic loci were 45.99%,40.57% and 41.04%,and the genetic diversities of the wild and the selected populations quantified by Shannon indexs were 0.181?8,0.180?7 and 0.177?4,respectively.The genetic distances and differentiation indexes between the wild and selected populations were large but they were very small between the ninth and the tenth generations of the selected populations 0.003?1 and 0.020?6 respectively.According to the results,with the increasing of selected generation,the selected populations showed that the genetic distances and genetic differentiation indexes became smaller,and that there was a convergence phenomeon implying that,this new variety had genetic stability.
[1]岑丰.我国水产养殖业的回顾与展望[J].现代渔业信息,1993,8(1):2-6.
[2]李健,刘萍,何玉英,等.中国对虾快速生长新品种“黄海1号”的人工选育[J].水产学报,2005,29(1):1-5.
[3]于安,路仁杰,王凤敏,等.“黄海1号”中国对虾2006年河北养殖调查报告[J].河北渔业,2007,3:20.
[4]Vos P,Hogers R,Bleeker M,et al.AFLP:a new technique forDNA fingerprinting[J].Nucleic Acids Research,1995,23(21):4407-4414.
[5]Chen S P,Bao Z M,Pan J,et al.Genetic diversity and specificmarkers in four scallop species,Patinopecten Yessoensis,Argopectenirradians,Chlamys nobilisandC.farreri[J].Acta OceanologicaSinica,2005,24(4):107-113.
[6]Congiu L,Dupanloup I,Patarnello T,et al.Identification of inter-specific hybrids by amplified fragment length polymorphism:the caseof sturgeon[J].Mol Ecol,2000,10:2355-2359.
[7]王维继,孔杰,董世瑞,等.中国明对虾AFLP分子标记遗传连锁图谱的构建[J].动物学报,2006,52(3):575-584.
[8]岳志芹,王维继,孔杰,等.用AFLP方法分析中国对虾抗病选育群体的遗传变异[J].水产学报,2005,29(1):13-19.
[9]王伟继,高焕,孔杰,等.利用AFLP技术分析中国明对虾的韩国南海种群和养殖群体的遗传差异[J].高技术通讯,2005,15(9):81-86.
[10]李朝霞,李健,何玉英,等.中国对虾人工选育快速生长群体不同世代间的AFLP分析[J].高技术通讯,2006,16(4):435-440.
[11]Garcia D K.Genetic diversity of cultured Penaeus vannamei shrimpusing three molecular genetic technique[J].Mol Mar Biol Biotech,1994,3:270.
[12]庄志猛,孔杰,石拓,等.日本对虾野生与养殖群体遗传多样性的RAPD分析[J].自然科学进展,2001,11(3):250-255.
[13]谭树华,王桂英,艾春香,等.斑节对虾养殖群体遗传多样性的同工酶和RAPD分析[J].中国水产科学,2005,12(6):702-707.
[14]李健,高天翔,柳广东,等.中国对虾人工选育群体的同工酶分析[J].海洋水产研究,2003,24(2):1-8.
[15]李健,刘萍,何玉英,等.中国对虾快速生长新品种“黄海1号”的人工选育[J].水产学报,2005,29(1):1-5.
[16]石拓,庄志猛,孔杰,等.中国对虾遗传多样性的RAPD分析[J].自然科学进展,2001,11(4):360-364.
[17]何玉英,刘萍,李健,等.中国明对虾第一代和第六代人工选育群体的遗传结构分析[J].中国水产科学,2004,11(6):572-575.
[18]何玉英,刘萍,李健,等.中国明对虾快速生长选育群体的RAPD分析[J].海洋水产研究,2005,26(4):8-13.
[19]张天时,王清印,刘萍,等.中国对虾(Fenneropenaeus chinensis)人工选育群体不同世代的微卫星分析[J].海洋与湖沼,2005,36(1):72-80.
[20]张天时,刘萍,李健,等.用微卫星DNA技术对中国对虾人工选育群体遗传多样性的研究[J].水产学报,2005,29(1):6-12.
[21]Wright S.The genetical structure of population[J].Annals of Eu-genics,1951,15:323-334.
[22]刘艳,李太武,孙修勤.栉孔扇贝稚贝的同工酶的基因表达[J].辽宁师范大学学报:自然科学版,2002,25(2):174-177.
[23]李太武,孙修勤,刘艳,等.栉孔扇贝种群的遗传变异分析[J].高技术通讯,2001:25-27.
[24]张志峰,马英杰,廖承义,等.中国对虾幼体发育阶段的同工酶研究[J].海洋学报,1997,19(4):63-71.
[25]杨翠华,王伟继,李鹏飞,等.红鳍东方6种同工酶的组织特异性及基因位点分析[J].海洋水产研究,2006,27(3):47-51.
[26]张岩,高天翔,刘曼红,等.顿吻黄盖鲽同工酶组织特异性及群体遗传结构的初步研究[J].中国海洋大学学报.自然科学版,2007,37(2):235-242.
[27]Pejic I,Ajmone Marsan P,Morgante M,et al.Comparative analy-sis of genetic similarity among maize inbred lines detected be RFLPs,RAPDs,SSRs,and AFLPs[J].Theor Appl Genet,1998,97:1248-1255.
[28]Garcia Mas J,Oliver M,Gomez Paniagua H,et al.ComparingAFLP,RAPD and RFLP markers for measuring genetic diversity inmelon[J].Theor Appl Genet,2000,101:860-864.
[29]王玲玲,宋林生,李红蕾,等.AFLP和RAPD标记技术在栉孔扇贝遗传多样性研究中的应用比较[J].动物学杂志,2003,38(4):35-39.
[30]杨东,余来宁.RAPD和AFLP在分析尼罗罗非鱼遗传多样性研究中的应用比较[J].江西农业学报,2006,18(2):1-4.
[31]Jones C J,Edwards K J,Castaglione S,et al.Reproducibility test-ing of RAPD,AFLP,and SSR markers in plants by a network ofEuropean Laboratories[J].Molecular Breeding,1997,3:381-390.
基本信息:
DOI：10.16441/j.cnki.hdxb.2008.06.011
中图分类号:S917.4
引用信息:
[1]安丽,刘萍,李健,等.“黄海1号”中国对虾不同世代间的AFLP分析[J].中国海洋大学学报(自然科学版),2008,No.166(06):921-926.DOI:10.16441/j.cnki.hdxb.2008.06.011.
基金信息:
国家科技支撑计划(2006BAD01A13);; 青岛市科技计划项目(07-2-3-5-jch);; 国家自然科学基金项目(40706052)资助
2008-11-15
2008-11-15