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以酪蛋白、明胶为主要蛋白源配制精制基础饲料,添加不同浓度梯度的还原型谷胱甘肽(GSH),饲料中GSH的含量分别为0、97.96、189.92、371.84、688.72和1 063.56 mg.kg-1,在流水式养殖系统中分别投喂初始体质量为(9.49±0.22)g的牙鲆(Paralichthys olivaceus)幼鱼,8周后观测各组牙鲆的生长情况及机体的抗氧化状态。结果表明,饲料中添加外源的GSH对牙鲆成活率没有显著影响(P>0.05);牙鲆增重率(WGR)在饲料GSH含量为371.84 mg.kg-1时达到最大值,显著高于其它各组(P<0.05);饲料GSH添加组牙鲆肝脏中GSH的含量显著高于零添加组(P<0.05);各处理组肝脏中丙二醛(MDA)的含量没有显著差异(P>0.05);肝脏中谷胱甘肽还原酶(GR)的活力随饲料中GSH含量的升高呈现先降低后上升的趋势,并在饲料中GSH含量为371.84 mg.kg-1时达到最低值;肝脏中超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GPx)活力没有受到饲料中GSH含量的显著影响(P>0.05);饲料中GSH含量为371.84 mg.kg-1时,肝脏中总抗氧化力(T-AOC)显著高于1 063.56 mg.kg-1组(P<0.05),谷胱甘肽-S-转移酶(GST)活力显著低于其它各组(P<0.05)。根据WGR的实验数据,利用折线模型得到牙鲆幼鱼饲料中GSH的适宜含量为368.92 mg.kg-1,过高或过低都会对生长造成负面影响,抑制机体抗氧化能力,增加机体氧化压力。
Abstract：The basal diet employed casein and gelatin as main protein sources and was supplemented with reduced glutathione(GSH) up to 0,97.96,189.92,371.84,688.72 and 1 063.56 mg·kg-1 diet.In the flow-through culture system,the Japanese flounders Paralichthys olivaceus(initial weight(9.49±0.22)g) were fed with graded levels of dietary GSH,respectively,for 8 weeks to evaluate their growth and anti-oxidative status.The results showed that survival rate was not significantly affected by dietary GSH levels(P>0.05).The average weight gain rate(WGR) in the treatment with 371.84 mg·kg-1 dietary GSH was significantly higher than those in other treatments(P<0.05).The hepatic GSH concentration in dietary GSH added groups was significantly higher than that in dietary GSH deprived group(control group)(P<0.05).No significant differences in hepatic malondiadehyde(MDA) concentration were found between GSH deprived and supplemented groups(P>0.05).The lowest value of hepatic GR activities was found in treatment with 371.84 mg·kg-1 dietary GSH(P<0.05).There were no significant differences in the activities of hepatic superoxide dismutase(SOD) and glutathione peroxidase(GPx) among the all treatments(P>0.05).In the group with 371.84 mg·kg-1 dietary GSH,the total anti-oxidative capability(T-AOC) was significantly higher than that in the group with 1 063.56 mg·kg-1 dietary GSH,however,the GST activity was significantly lower than those in the other groups(P<0.05).Based on the WGR data in the present experiment,a broken-line model was employed to calculate the optimal levels of dietary GSH,which was 368.92 mg·kg-1 diet.Deficient or overdose of dietary GSH could cause negative effects on the growth and anti-oxidative capacity of Paralichthys olivaceus.
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基本信息:
DOI：10.16441/j.cnki.hdxb.2011.04.009
中图分类号:S963.73
引用信息:
[1]王芳倩,张文兵,麦康森,等.饲料中添加还原型谷胱甘肽对牙鲆生长和抗氧化能力的影响[J].中国海洋大学学报(自然科学版),2011,41(04):51-56.DOI:10.16441/j.cnki.hdxb.2011.04.009.
基金信息:
国家高技术研究发展计划项目(2007AA09Z437)资助
2011-04-15
2011-04-15