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对青岛产仿刺参体内皂苷类成分进行分离及结构鉴定,确定其皂苷的主要组成及比例。应用大孔树脂,正相硅胶柱层析和半制备型HPLC对青岛产仿刺参体内的皂苷成分进行分离纯化,根据化合物的理化性质和波谱数据鉴定其结构。共分离并鉴定3种三萜皂苷类化合物,分别为Cladoloside B(1),Holotoxin A1(2)和Holotoxin B1(3)。青岛海域仿刺参体内的皂苷组成主要为Cladoloside B(1),Holotoxin A1(2)和Holotoxin B1(3),比例约为1∶4.5∶1.7。
Abstract：To study the constituents and proportion of triterpene glycosides from sea cucumber S.japonicus of Qingdao,Triterpene glycosides were separated and purified by macroporous resin,Si-gel chromatography and semi-preparative HPLC.Three triterpene glycosides were found and were identified as cladoloside B(1),holotoxin A1(2) and holotoxin B1(3) with an approximate ratio of 1∶4.5∶1.7 based on the chemical evidence and spectral data.
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基本信息:
DOI：10.16441/j.cnki.hdxb.2010.08.009
中图分类号:S968.9
引用信息:
[1]薛长湖,于林芳,董平,等.青岛仿刺参中3种海参皂苷的分离纯化及结构鉴定[J].中国海洋大学学报(自然科学版),2010,40(08):60-65.DOI:10.16441/j.cnki.hdxb.2010.08.009.
基金信息:
国家科技支撑计划项目(2008BAD94B05)资助
2010-08-15
2010-08-15