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为实现循环海水养殖系统中硝酸盐的经济、高效去除，采用固定化好氧反硝化菌(Marinobacter sp. strain B108)有效解决菌剂持留问题，进而提升好氧环境下硝酸盐去除效率。本研究以Marinobacter sp. strain B108为研究对象，采用聚乙烯醇(PVA)、海藻酸钠(SA)、茶渣粉为固定化载体材料，探究并优化菌球固定化材料配比，在此基础上进一步研究固定化好氧反硝化菌球的理化特征及脱氮动力学特性。结果表明，固定化材料最佳配比为PVA 10.103%、SA 1.548%、茶渣粉0.732%。最佳配比条件下固定化菌球机械强度高，耐受机械剪切力强；当初始NO■-N浓度为100 mg/L时，固定化菌球7 h内NO■-N去除率可达98.63%。固定化菌球对NO■-N去除动力学过程可用Monod模型拟合，拟合得出最大比去除速率Vmax为5.041 mg/(L·g·h),半饱和常数Km为24.810 mg/L;固定化菌球对NO■-N去除动力学过程可用Andrews模型拟合，拟合得出Vmax为0.790 mg/(L·g·h),Km为23.783 mg/L,抑制常数Ki为0.092 mg/L。研究可为好氧反硝化菌在循环海水养殖系统硝酸盐处理中的应用奠定基础。
Abstract：Immobilized aerobic denitrifying bacteria(Marinobacter sp. strain B108) can solve the problem of bacterial retention and improve the nitrate removal efficiency under aerobic environment, thus removing nitrate from marine recirculating aquaculture systems(MRAS) economically and efficiently. Polyvinyl alcohol(PVA), sodium alginate(SA) and tea residue powder were used as support materials to immobilize Marinobacter sp. strain B108. The ratios of immobilized materials were explored and optimized, and the physicochemical characteristics and denitrification kinetics of immobilized aerobic denitrifying bacterial pellets were further investigated on the basis of the optimal immobilized materials ratio. The optimum ratios of support materials were 10.103% PVA, 1.548% SA, and 0.732% tea residue powder. Under the optimum conditions, the immobilized bacterial pellets had high mechanical strength and strong resistance to mechanical shear, and the removal efficiency of NO■-N within 7 h was 98.63% as initial influent NO■-N concentration was 100 mg/L. The kinetic process of NO■-N removal could be fitted by Monod model, and the Vmax and Km were 5.041 mg/(L·g·h) and 24.810 mg/L, respectively. The kinetic process of NO■-N removal could be fitted by Andrews model, and the Vmax, Km and Ki were 0.790 mg/(L·g·h), 23.783 mg/L, and 0.092 mg/L, respectively. The conclusions could lay a good foundation for aerobic denitrifying bacteria practical application in MRAS for nitrate removal treatment.
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基本信息:
DOI：10.16441/j.cnki.hdxb.20240175
中图分类号:X172;X714
引用信息:
[1]王文鑫,林久淑,聂新华,等.好氧反硝化菌Marinobacter sp. strain B108固定化及脱氮动力学研究[J].中国海洋大学学报(自然科学版),2025,55(09):103-113.DOI:10.16441/j.cnki.hdxb.20240175.
基金信息:
国家自然科学基金项目(51978636)资助~~
2024-04-23
2024
2024-05-16
2024
2024-05-11
1
2025-08-26
2025-08-26