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研究开发了1种多倍体诱导的新方法,采用低渗抑制受精卵第2极体(PB2)的释放诱导虾夷扇贝(Patinopecten yessoensis)三倍体。结果表明:以低渗盐度S=12,在第一个PB2出现时开始处理,持续处理20min,诱导率可高达(84.74±3.82)%;三倍体处理组的幼虫比对照组具有明显的生长优势,但是幼虫存活率低于二倍体对照组。
Abstract：A new method of polyploid induction was developed and used in scallop Patinopecten yessoensis for triploid induction by inhibiting polar bodyⅡ(PB2)releasing with hypotonic treatment. Results showed when the fertilized eggs were treated 20 min with a hypotonic solution of 12 salinity at the time when the first PB2 was observed, can yield that the highest triploidy rate which was up to 84.74%±3.82%. The induced triploid larvae appeared much advantageous in growth, but the survival rate was lower than that of the diploid controls.
[1]Stanley J G,Allen S KJr,Hidu H.Ployploidyinducedinthe Ameri-can oysterCrassostrea virginicawith Cytochalasin B[J].Aquacul-ture,1981,23:1-10.
[2]王昭萍,王如才,于瑞海,等.多倍体贝类的生物学特性[J].青岛海洋大学学报,1998,28(3):309-403.
[3]常亚青,王子臣,杨旦光.贝类多倍体育种研究现状[J].水产科学,2002,21(1):31-36.
[4]Downing S L,Allen S K Jr.Induced triploid in the pacific oysters Crassostrea vergigas,opti mal treatment with Cytochalasin B depend on temperature[J].Aquaculture,1987,61:1-15.
[5]Desrosiers R R,Ggerard A,Peignon J M,et al.Anovel method to produce triploids in bivalve mollusc by the use of6-Di methyla minop-urine[J].J Exp MarBiol Rcol,1993,170(1):29-43.
[6]于瑞海,王昭萍,王如才,等.三种化学诱导剂诱导太平洋牡蛎三倍体的比较研究[J].青岛海洋大学学报,2000,30(4):589-592.
[7]Araik,Naito F,Fujino K.Triploidization of the Pacific abalone with temperature and pressure treatments[J].Bull Japan Soc Sci Fish,1986,52(3):417-422.
[8]于瑞海,王昭萍,王如才,等.两种方法诱导太平洋牡蛎三倍体在生产上的应用效果[J].海洋湖沼通报,2003,1:57-61.
[9]Guo X,Allen S K.All-triploid pacific oysters,Crassostrea gigas(Thunberg)produced by mating tetraploids and diploids[J].Aqua-culture,1996,142:149-161.
[10]杨蕙萍,李莉,郭希明.细胞松弛素B诱导虾夷扇贝多倍体初探[J].动物学报,2001,(4):459-464.
[11]常亚青,相建海,张国范,等.虾夷扇贝三倍体诱导与培育技术的研究[J].中国水产科学,2001,(1):18-21.
[12]王子臣,毛连菊,陈来钊,等.温度休克诱导栉孔扇贝和虾夷扇贝三倍体的初步研究[J].大连水产学院学报,1990,5(3-4):1-6.
[13]于瑞海,王如才,王昭萍,等.不同卵密度对太平洋牡蛎三倍体诱导效果影响的研究[J].海洋湖沼通报,2002,(2):57-61.
[14]容寿柏,李一民,刘绍琼,等.用冷热休克诱导四倍体近江牡蛎[J].湛江水产学院学报,1992,12(2):18-21.
[15]田传远,梁英,王如才,等.6-DMAP诱导太平洋牡蛎三倍体孵化率和D幼畸形率与三倍体诱导率的关系[J].青岛海洋大学学报,1998,28(3):421-425.
[16]田传远,王如才,梁英,等.6-DMAP诱导太平洋牡蛎三倍体抑制受精卵第二极体释放[J].中国水产科学,1999,6(2):1-4.
基本信息:
DOI：10.16441/j.cnki.hdxb.2009.02.004
中图分类号:S968.31
引用信息:
[1]王昭萍,赵婷,于瑞海,等.一种新方法——低渗诱导虾夷扇贝三倍体的研究[J].中国海洋大学学报(自然科学版),2009,39(02):193-196.DOI:10.16441/j.cnki.hdxb.2009.02.004.
基金信息:
国家自然科学基金项目(30771622);; 国家高技术研究发展规划项目(2006AA10A401)资助
2009-03-15
2009-03-15