| 286 | 9 | 336 |
| 下载次数 | 被引频次 | 阅读次数 |
利用扫描电子显微镜观察中国西沙群岛的玉足海参(Holothuria leucospilota)、虎纹海参(Holothuria pervicax)、黑赤星海参(Holathuria cinerascens)、黄疣海参(Holathuria hilla)、格皮氏海参(Pearsonothuria graeffei)、糙刺参(Stichopus horrens)6种海参的背脊中部和腹部的骨片,获得了6种海参骨片的超显微结构图像。研究发现,6种海参的骨片分为桌形体、扣状体、穿孔板、杆状体、C形体、花纹样体、颗粒体7种类型。研究发现了新的海参骨片:玉足海参的穿孔板;虎纹海参的具横梁桌形体;黑赤星海参的II型桌形体;黄疣海参的II型扣状体;格皮氏海参的饼干形穿孔板和II型花纹样体;糙刺参中部具扇形突起的杆状体和I型花纹样体。本研究进一步丰富了我国海参骨片形态学研究的资料。
Abstract：The ossicles of six holothurians in Xisha Islands of China have been observed with scanning electronic microscope(SEM).Some ultrastructure images were taken,so that the details of those micro ossicles could be clearly observed.Seven types of ossicles were found,namely table,button,rod,plate,C-shaped,rosette and grain.Comparing with the previous reports,some new ossicles were discovered,including the plate in Holothuria leucospilota,a type of table in Holothuria pervicax,a type of table in Holathuria cinerascens,a type of button in Holathuriahilla,a type of rosette and a type of plate in Pearsonothuria graeffei,a type of rod and a type of rosette in Stichopus horrens.This study provided new data of ossicles in the form of ultrastructure images.And it is convenient to do the morphological identification for sea cucumbers in China with the aid of these types of ultrastructure.
[1]Pagad S,Hayes K,Katsanevakis S,et al.World Register of Introduced Marine Species(WRIMS)[EB/OL].(2014-08-01)[2016-01-05]http://www.marinespecies.org:VLIZ.
[2]廖玉麟,肖宁.中国海棘皮动物的种类组成及区系特点[J].生物多样性,2011,19(6):729-736.Liao Y,Xiao N.Species composition and faunal characteristics of echinoderms in China seas[J].Biodiversity Science,2011,19(6):729-736.
[3]崔桂友,赵廉.食用海参的名称与种类鉴别[J].扬州大学烹饪学报,2000,17(3):13-18.Cui G,Zhao L.Names and species identifying of edible sea cucumber in China[J].Cuisine Journal of Yangzhou University,2000,17(3):13-18.
[4]郭盈莹,丁燕,徐飞飞,等.海参中主要生物活性成分研究进展[J].食品科学,2014,15:68.Guo Y,Ding Y,Xu F,ET AL.Progress in research on main bioactive constituents of Sea cucumber[J].Food Science,2014,15:68.
[5]廖玉麟.我国的海参[J].生物学通报,2001,36(9):1-3.Liao Y.Sea cucumbers of China[J].Bulletin of Biology,2001,36(9):1-3.
[6]Purcell S W,Samyn Y,Conand C.Commercially important sea cucumbers of the world[C].//FAO.Species Catalogue for Fishery Purposes No.6.Rome:FAO,2012:3-4.
[7]Stricker S A.The ultrastructure and formation of the calcareous ossicles in the body wall of the sea cucumber Leptosynapta clarki(Echinodermata,Holothuroida)[J].Zoomorphology,1985,105(4):209-222.
[8]Kerr A M,Janies D A,Clouse R M,et al.Molecular phylogeny of coral-reef sea cucumbers(Holothuriidae:Aspidochirotida)based on 16S mitochondrial ribosomal DNA sequence[J].Marine Biotechnology,2005,7(1):53-60.
[9]Kamarudin K R,Rehan M M.Morphological and molecular identification of Holothuria(Merthensiothuria)leucospilotaand Stichopus horrens from Pangkor Island,Malaysia[J].Tropical Life Sciences Research,2015,26(1):87.
[10]廖玉麟.中国动物志:棘皮动物门.海参纲[M].北京:科学出版社,1997.Liao Y.Fauna Sinica:Phylum Echinodermata.Class Holothuroidea[M].Beijing:Science Press,1997.
[11]赵世民.台湾礁岩海岸的海参[M].台中:“国立”自然科学博物馆,1998.Zhao S.Shallow-Water Sea Cucumber of Taiwan[M].Taizhong:National Museum of Natural Science,1998.
[12]Toral-Granda M V,Rasolofonirina R,Jangoux M,et al.The use of calcareous spicules for the identification of the Galápagos sea cucumber Isostichopus fuscus in the international market[J].SPC Beche de Mer Information Bulletin,2005,22:3-5.
[13]李赟,费来华,陈家鑫.十五种海参骨片的形态学研究[J].中国海洋大学学报(自然科学版),2008,38(2):211-216.Li Y,Fei L,Chen J.The Morphology of ossicles of 15commercial Holothurians[J].Periodical of Ocean University of China,2008,38(2):211-216.
[14]文菁,张吕平,胡超群,等.中国热带6种海参骨片的种类和形态研究[J].海洋学报,2009,31(2):139-145.Wen J,Zhang L,Hu C,et al.Classification an morphology of ossicles of six tropical holothurians in the South China Sea[J].Acta Oceanologica Sinica,2009,31(2):139-145.
[15]范嗣刚,胡超群,张吕平,等.西沙群岛8种热带海参的骨片形态特征[J].热带海洋学报,2010,29(4):148-153.Fan S,Hu C,Zhang L,et al.Morphological character of ossicles in eight tropical holothurians in the Xisha Islands of China[J].Journal of Tropical Oceanography,2010,29(4):148-153.
[16]尤丰,黄晖,张浴阳.海南岛海域枝手目海参新种描述及初步系统进化[J].科学通报,2013,58(17):1604-1609.You F,Huang H,Zhang Y Y.Description of a new cucumariid species from Hainan Island and preliminary study of phylogenetic relation(in Chinese)[J].Chin Sci Bull(Chin Ver),2013,58:1604-1609.
[17]文菁,曾玲,王雁,等.运用形态学和分子生物学方法鉴定市场中伪劣海参产品[J].湛江师范学院学报,2012,3:95-99.Wen J,Zeng L,Wang Y,et al.Morphological and molecular methods to identify genuine and fake sea cucumber products[J].Journal of Zhanjing Normal College,2012,3:95-99.
[18]张庆军.扫描电子显微镜的应用[J].河北理工学院学报,1998,20(3):103.Zhang Q.Application of scanning electron microscope[J].Journal of Hebei Institute of Technology,1998,20(3):103.
[19]朱晓丽.显微技术在生物学中的应用[J].生物技术世界,2015,12:271-272.Zhu X.Application of in Biology of microtechnic[J].Biotech World,2015,12:271-272.
基本信息:
DOI：10.16441/j.cnki.hdxb.20160012
中图分类号:S917.4
引用信息:
[1]姚旺,顾炎斌,吴古远,等.西沙群岛6种海参骨片的超显微结构研究[J],2017,47(01):76-81.DOI:10.16441/j.cnki.hdxb.20160012.
基金信息:
海洋公益专项项目(201505004);; 中央高校基本科研业务费项目(201262037)资助~~