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为探讨饲料中添加一定比例的南极磷虾粉(Antarctic krill meal)在凡纳滨对虾(Litopenaeus vannamei)亲虾营养强化期间的作用,以筛选出在亲虾营养强化期间适合的人工配合饲料。用3种比例不同的磷虾粉人工配合饲料(0、10%和20%,对应的编号为K0、K10和K20)和沙蚕(对照组)分别对凡纳滨对虾进行60天的营养强化。营养强化后对每个处理组的雌性亲虾进行单侧眼柄摘除手术,亲虾进入繁殖产卵阶段,通过人工授精操作使亲虾交配、产卵,收集产卵相关数据。研究表明,K10和K20组的亲虾产卵量、受精卵孵化率和无节幼体变态率与对照组无显著差异(P>0.05);K20组的亲虾产卵率、多次产卵率和卵径大小显著高于对照组(P<0.05),K10组的亲虾产卵率显著高于对照组(P<0.05)。脂肪酸组成分析表明,K10和K20组与沙蚕组的多不饱和脂肪酸组成相同(包括C20:5n-3、C22:6n-3、C20:4n-6等);各处理组的卵脂肪酸组成与饲料的脂肪酸组成在一定程度上正相关。研究结果表明,添加10%~20%南极磷虾粉的人工配合饲料可作为凡纳滨对虾亲虾营养强化期间的饵料。
Abstract：To study the function of Antarctic krill meal to Litopenaeus vannamei broodstocks in their nutrient enrichment phase,we designed three kinds of artificial formula diets with different krill meal content(0,10%,20%),which were numbered Diet K0,Diet K10 and Diet K20,respectively,in this study.Three kinds of artificial formula diets were used to feed L.vannamei broodstocks in their nutrient enrichment phase for 60 days,while the fresh bait,Nereididae,was used as the control.After 60days' nutrient enrichment phase,the females were subjected to unilateral eyestalk ablation,then mature females were subjected to artificial fertilization.The number of eggs(fecundity)was estimated,as well as the egg hatchability and metamorphism rate.In addition,the egg diameter and fatty acids profile were also measured.Fatty acids profile of the three kinds of artificial formula diets was analyzed,so did the fresh bait.The spawning rate,continuous spawning rate and average spawning times in four treatment groups were calculated.The results showed that the female broodstocks in control group had the highest fecundity,but had no significant difference(P>0.05)with Diet K10 and Diet K20 group.There was no significant difference(P>0.05)between the four groups about egg hatchability.As to metamorphism rate,the control group,Diet K10 and Diet K20 group had no significant difference(P>0.05)with each other,and had significant difference(P<0.05)with Diet K0 group.Diet K0,Diet K10 and Diet K20 groups had higher spawning rate,continuous spawning rate and average spawning times than the control group.Diet K20 group had the maximal egg diameter,had no significant difference(P>0.05)with Diet K10 group,and had significant difference(P<0.05)with Diet K0 group and control group.Fatty acids profile analysis showed that there was a positive correlation between the fatty acids profiles in eggs and in diets partly.Furthermore,the egg fatty acids profiles in Diet K10 and Diet K20 groups had the similar even superior status than that in control group.We concluded that Diet K10 and Diet K20 which contained certain content krill meal(10% ~20%)could be the excellent artificial formula diets in L.vannamei broodstocks' nutrient enrichment phase.
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基本信息:
DOI：10.16441/j.cnki.hdxb.20160370
中图分类号:S968.22
引用信息:
[1]张玉玲,孔杰,梁萌青,等.饲料中添加南极磷虾粉对营养强化阶段凡纳滨对虾亲虾产卵的影响[J],2017,47(06):69-77.DOI:10.16441/j.cnki.hdxb.20160370.
基金信息:
中国水产科学研究院黄海水产研究所中央级公益性科研院所基本科研业务费专项(20603022016006);; 山东省重点研发计划项目“凡纳滨对虾耐盐碱品种选育及健康养殖技术研究”(2016GSF115030)资助~~
2016-11-02
2016
2017-01-20
2017-03-21
2017
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