| 1,151 | 17 | 143 |
| 下载次数 | 被引频次 | 阅读次数 |
本研究针对集约化海水养殖引起的氮、磷污染问题，选用海水小球藻(Chlorella sp.)和分离自养殖池塘的芽孢杆菌(Bacillus sp.)SD148BN14构建了一种微藻-细菌复合体系。该复合体系能够去除合成废水中90.66%的总氮和93.04%的总磷，氮、磷去除性能优于单独使用小球藻或菌株SD148BN14。藻-菌复合体系产生的生物量达0.44 g/L,且复合体系生物量中蛋白质和脂质占比显著高于小球藻生物量中二者占比。小球藻与菌株SD148BN14在细胞生长、光合活性和物质交换等方面表现出以协同为主的相互作用关系。在温度20～35℃、盐度10～40、光照强度4 000～16 000 lx、pH6～9、碳氮比1～10、氮磷比4～10、氮浓度1～20 mg/L条件下，藻-菌复合体系具有稳定、高效的氮去除能力。使用藻-菌复合体系能够去除实际海水养殖水中92%以上的总氮和总磷，去除实际海水养殖废水中95%以上的总氮和总磷。生物安全性实验初步验证了菌株SD148BN14应用于养殖环境的可行性。研究结果表明，该海水小球藻-芽孢杆菌复合体系具有优异的氮磷去除性能和生物量生产能力，兼具良好的海水养殖环境适应性，具备治理海水养殖氮磷污染的潜力。
Abstract：In view of nitrogen and phosphorus pollutions caused by intensive mariculture, in this study, Chlorella sp. and Bacillus sp. SD148 BN14 were used to construct a new microalgae-bacteria consortium. The consortium could remove 90.66% of total nitrogen and 93.04% of total phosphorus from synthetic mariculture wastewater and the nitrogen and phosphorus removal efficiencies were better than those of either microalgae-only or bacteria-only. The biomass production of the microalgae-bacteria consortium reached 0.44 g/L. The protein and lipid proportions in biomass of the consortium were higher than those of the microalgae-only. The interactions between Chlorella sp. and Bacillus sp. SD148 BN14 were mainly synergistic in cell growth, photosynthetic activity and substance exchange. The microalgae-bacteria consortium had stable and efficient removal ability of nitrogen at temperatures varying between 20 and 35 ℃, salinities between 10 and 40, light intensities between 4 000 and 16 000 lx, acidities between pH 6 and pH 9, carbon-to-nitrogen ratios between 1 and 10, nitrogen-to-phosphorus ratios between 4 and 10, and nitrogen concentrations between 1 and 20 mg/L. The consortium could remove more than 92% of total nitrogen as well as total phosphorus from real mariculture water and more than 95% of total nitrogen as well as total phosphorus from real mariculture wastewater. Biosafety experiments preliminarily confirmed the feasibility of Bacillus sp. SD148 BN14 used in mariculture environment. The excellent removal performance of nitrogen and phosphorus, capability of biomass production and adaptability to mariculture environment made the Chlorella sp.-Bacillus sp. SD148 BN14 consortium a potential use for nitrogen and phosphorus pollution treatment in mariculture.
[1] Yang P,Lai D Y F,Jin B S,et al.Dynamics of dissolved nutrients in the aquaculture shrimp ponds of the Min River estuary,China:Concentrations,fluxes and environmental loads[J].Science of the Total Environment,2017,603-604:256-267.
[2] 鲁泉，陈新军.改革开放40年来中国渔业产业发展及十四五产量预测[J].上海海洋大学学报，2021,30(2):339-347.Lu Q,Chen X J.Development of Chinese fishery industry in 40 years of reform and opening up and production forecast in the 14th five-year plan[J].Journal of Shanghai Ocean University,2021,30(2):339-347.
[3] Hou P F,Sun X L,Fang Z M,et al.Simultaneous removal of phosphorous and nitrogen by ammonium assimilation and aerobic denitrification of novel phosphate-accumulating organism Pseudomonas chloritidismutans K14[J].Bioresource Technology,2021,340:125621.
[4] Mohsenpour S F,Hennige S,Willoughby N,et al.Integrating micro-algae into wastewater treatment:A review[J].Science of the Total Environment,2021,752:142168.
[5] Malibari R,Sayegh F,Elazzazy A M,et al.Reuse of shrimp farm wastewater as growth medium for marine microalgae isolated from Red Sea-Jeddah[J].Journal of Cleaner Production,2018,198:160-169.
[6] Su Z P,Li Y,Pan L Q,et al.Nitrogen removal performance,quantitative detection and potential application of a novel aerobic denitrifying strain,Pseudomonas sp.GZWN4 isolated from aquaculture water[J].Bioprocess and Biosystems Engineering,2021,44(6):1237-1251.
[7] Kouzuma A,Watanabe K.Exploring the potential of algae/bacteria interactions[J].Current Opinion in Biotechnology,2015,33:125-129.
[8] Ji X Y,Jiang M Q,Zhang J B,et al.The interactions of algae-bacteria symbiotic system and its effects on nutrients removal from synthetic wastewater[J].Bioresource Technology,2018,247:44-50.
[9] Zhang M Y,Pan L Q,Liu L P,et al.Phosphorus and nitrogen removal by a novel phosphate-accumulating organism,Arthrobacter sp.HHEP5 capable of heterotrophic nitrification-aerobic denitrification:Safety assessment,removal characterization,mechanism exploration and wastewater treatment[J].Bioresource Technology,2020,312:123633.
[10] Su Y Y.Revisiting carbon,nitrogen,and phosphorus metabolisms in microalgae for wastewater treatment[J].Science of the Total Environment,2020,762:144590.
[11] Li Y T,Wang Y F,Gao Y Z,et al.Seawater toilet flushing sewage treatment and nutrients recovery by marine bacterial-algal mutualistic system[J].Chemosphere,2018,195:70-79.
[12] Saravanan A,Kumar P S,Varjani S,et al.A review on algal-bacterial symbiotic system for effective treatment of wastewater[J].Chemosphere,2021,271:129540.
[13] Ferro L,Colombo M,Posadas E,et al.Elucidating the symbiotic interactions between a locally isolated microalga Chlorella vulgaris and its co-occurring bacterium Rhizobium sp.in synthetic municipal wastewater[J].Journal of Applied Phycology,2019,31(4):2299-2310.
[14] Zhang Z S,Guo L,Liao Q R,et al.Bacterial-algal coupling system for high strength mariculture wastewater treatment:Effect of temperature on nutrient recovery and microalgae cultivation[J].Bioresource Technology,2021,338:125574.
[15] Lananan F,Hamid S H A,Din W N S,et al.Symbiotic bioremediation of aquaculture wastewater in reducing ammonia and phosphorus utilizing Effective Microorganism (EM-1) and microalgae (Chlorella sp.)[J].International Biodeterioration & Biodegradation,2014,95:127-134.
[16] Wang Y Y,Wang S Y,Sun L Q,et al.Screening of a Chlorella-bacteria consortium and research on piggery wastewater purification[J].Algal Research,2020,47:101840.
[17] Huang F,Pan L Q,He Z Y,et al.Identification,interactions,nitrogen removal pathways and performances of culturable heterotrophic nitrification-aerobic denitrification bacteria from mariculture water by using cell culture and metagenomics[J].Science of the Total Environment,2020,732:139268.
[18] Zhang M Y,Pan L Q,Su C,et al.Simultaneous aerobic removal of phosphorus and nitrogen by a novel salt-tolerant phosphate-accumulating organism and the application potential in treatment of domestic sewage and aquaculture sewage[J].Science of the Total Environment,2021,758:143580.
[19] Wang X F,Miao J J,Pan L Q,et al.Toxicity effects of p-choroaniline on the growth,photosynthesis,respiration capacity and antioxidant enzyme activities of a diatom,Phaeodactylum tricornutu[J].Ecotoxicology and Environmental Safety,2019,169:654-661.
[20] Liu X,Steele J C,Meng X Z.Usage,residue,and human health risk of antibiotics in Chinese aquaculture:A review[J].Environmental Pollution,2017,223:161-169.
[21] Zhao Y T,Zhang X X,Zhao Z H,et al.Metagenomic analysis revealed the prevalence of antibiotic resistance genes in the gut and living environment of freshwater shrimp[J].Journal of Hazar-dous Materials,2018,350:10-18.
[22] Han W,Mao Y F,Wei Y P,et al.Bioremediation of aquaculture wastewater with algal-bacterial biofilm combined with the production of selenium rich biofertilizer[J].Water,2020,12(7):2071.
[23] Liu H,Lu Q,Wang Q,et al.Isolation of a bacterial strain,Acinetobacter sp.from centrate wastewater and study of its cooperation with algae in nutrients removal[J].Bioresource Technology,2017,235:59-69.
[24] He Q N,Yang H J,Wu L,et al.Effect of light intensity on physiological changes,carbon allocation and neutral lipid accumulation in oleaginous microalgae[J].Bioresource Technology,2015,191:219-228.
[25] Peng H X,De-Bashan L E,Higgins B T.Comparison of algae growth and symbiotic mechanisms in the presence of plant growth promoting bacteria and non-plant growth promoting bacteria[J].Algal Research,2021,53:102156.
[26] Natrah F M I,Bossier P,Sorgeloos P,et al.Significance of microalgal-bacterial interactions for aquaculture[J].Reviews in Aquaculture,2014,6(1):48-61.
[27] Cao M,Kang J M,Gao Y M,et al.Optimization of cultivation conditions for enhancing biomass,polysaccharide and protein yields of Chlorella sorokiniana by response surface methodology[J].Aquaculture Research,2020,51(6):2456-2471.
[28] Rasouli Z,Valverde-Pérez B,D’este M,et al.Nutrient recovery from industrial wastewater as single cell protein by a co-culture of green microalgae and methanotrophs[J].Biochemical Engineering Journal,2018,134:129-135.
[29] Guccione A,Biondi N,Sampietro G,et al.Chlorella for protein and biofuels:From strain selection to outdoor cultivation in a Green Wall Panel photobioreactor[J].Biotechnology for Biofuels and Bioproducts,2014,7(1):84.
[30] Ramanan R,Kim B H,Cho D H,et al.Algae-bacteria interactions:Evolution,ecology and emerging applications[J].Biotechnology Advances,2016,34(1):14-29.
[31] Chang Z Q,Neori A,He Y Y,et al.Development and current state of seawater shrimp farming,with an emphasis on integrated multi-trophic pond aquaculture farms,in China-a review[J].Reviews in Aquaculture,2020,12(4):2544-2558.
[32] Kumlu M,Kumlu M,Turkmen S.Combined effects of temperature and salinity on critical thermal minima of pacific white shrimp Litopenaeus vannamei (Crustacea:Penaeidae)[J].Journal of Thermal Biology,2010,35(6):302-304.
[33] Huang F,Pan L Q,He Z Y,et al.Culturable heterotrophic nitrification-aerobic denitrification bacterial consortia with cooperative interactions for removing ammonia and nitrite nitrogen in mariculture effluents[J].Aquaculture,2020,523:735211.
[34] Hoang M N,Nguyen P N,Bossier P.Water quality,animal performance,nutrient budgets and microbial community in the biofloc-based polyculture system of white shrimp,Litopenaeus vannamei and gray mullet,Mugil cephalus[J].Aquaculture,2020,515:734610.
[35] Xu W J,Xu Y,Huang X S,et al.Addition of algicidal bacterium CZBC1 and molasses to inhibit cyanobacteria and improve microbial communities,water quality and shrimp performance in culture systems[J].Aquaculture,2019,502:303-311.
[36] Huang F,Pan L Q,He Z Y,et al.Heterotrophic nitrification-aerobic denitrification characteristics and antibiotic resistance of two bacterial consortia from Marinomonas and Halomonas with effective nitrogen removal in mariculture wastewater[J].Journal of Environmental Management,2021,279:111786.
基本信息:
DOI：10.16441/j.cnki.hdxb.20220005
中图分类号:X714;X17
引用信息:
[1]刘丽平,李赟,潘鲁青,等.海水小球藻-芽孢杆菌复合体系去除海水养殖中氮磷污染的研究[J],2022,52(11):43-55.DOI:10.16441/j.cnki.hdxb.20220005.
基金信息:
广东省重点领域研究发展计划项目(2020B0202010009)资助~~