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采用潮汐流人工湿地，研究不同淹没/空闲时长比(F/R)对含盐废水脱氮效果的影响，分析运行周期内无机氮的沿程变化，利用高通量测序技术阐析湿地内微生物群落结构和脱氮功能菌的分布特征。结果表明，改变F/R对湿地的硝化作用影响不大，NH+_4-N去除率无显著差异(p>0.05),平均去除率达85.37%;F/R对总无机氮(TIN)去除有明显影响(p<0.05),较长的淹没时间促进了湿地的反硝化效果，进而提高了TIN去除率；F/R为15.5 h/6.5 h时，TIN去除率最高(62.94±3.70)%。运行周期内，淹没初期NH+_4-N浓度迅速降低，NO~-3-N开始积累，NH+_4-N的氧化主要发生在深度0～27 cm区域，在湿地内存在同步硝化反硝化过程(SND,Simultaneous nitrification and denitrification)。湿地内检测到的脱氮功能菌主要是氨氧化古菌Thaumarchaeota、氨氧化细菌Nitrosomonas、亚硝酸盐氧化细菌Nitrospira、反硝化细菌Dokdonella和Nakamurella,硝化细菌的丰度远低于反硝化细菌。
Abstract：Tidal flow constructed wetlands were established to study the influence of flood/rest time ratio(F/R) on the inorganic nitrogen removal efficiency of saline wastewater. Meanwhile, the temporal and spatial variation of pollutants in a operation cycle were analyzed. High-throughput sequencing technology was used to illustrate the microbial community structure and the distribution characteristics of functional bacteria related to nitrogen removal. The results showed that F/R had little influence on the nitrification of wetland, and there was no significant difference in NH+_4-N removal rate(p>0.05), with an average removal rate of 85.37%. F/R had a significant effect on the total inorganic nitrogen(TIN) removal efficiency(p<0.05), and the longer flood time promoted the denitrification process of the wetland, thus contribute the removal efficiency of TIN. The highest TIN removal efficiency(62.94±3.70)% were achieved when F/R was 15.5 h/6.5 h. In a operation cycle, the concentration of NH+_4-N falling rapidly in the initial period, NO~-3-N were accumulate in the beginning of the flood, the nitrification process mainly takes place in 0～27 cm(along the depth) area. The simultaneous nitrification and denitrification process(SND) was existed in the wetland. The functional bacteria related to nitrogen removal detected in the wetland are mainly Thaumarchaeota(ammonia-oxidizing archaea, AOA), Nitrosomonas(ammonia-oxidizing bacterium, AOB), Nitrospira(nitrite oxidizing bacteria, NOB), and Dokdonella and Nakamurella(denitrifying bacteria, DNB). The abundance of denitrifying bacteria is much lower than that of denitrifying bacteria.
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基本信息:
DOI：10.16441/j.cnki.hdxb.20210075
中图分类号:X703
引用信息:
[1]张晓雨,王福浩,夏振刚,等.潮汐运行方式对人工湿地处理含盐废水脱氮性能的影响[J],2022,52(07):107-117.DOI:10.16441/j.cnki.hdxb.20210075.
基金信息:
中央高校基本科研业务费专项(201964003)资助~~
2021-02-22
2021
2021-04-28
2021
2021-04-15
1