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考察了以氢气作为电子供体和厌氧污泥作为接种污泥培养驯化氢自养高氯酸盐还原菌的过程。结果表明,在高氯酸盐的起始控制浓度约为300mg/L和厌氧条件下,采用周期运行培养方式,随着培养驯化时间的增加而逐渐缩短,高氯酸根去除率达99%所需时间从最初的8天最终稳定在1天,未发现中间产物的ClO-3和ClO-2的积累,全部被还原为氯离子。对不同阶段的样品进行PCR-DGGE图谱和相似系数分析,发现随着反应的持续进行,微生物种群结构发生了演替变化,证实污泥微生物生态能够迅速进行优胜劣汰的筛选,调整内部微生物种群结构,从而达到适应环境的目的。
Abstract：Enrichment and cultivation of Hydrogen autotrophic perchlorate-reducing bacteria were investigated by using Hydrogen as electron and anaerobic sludge as sludge seeding.Under the anaerobic condition and the initial consistency control at about 300mg/L using period operation method,the requisite time of perchlorate removal efficiency above 99% gradually decreased with the time increasing of enrichment and cultivation,which varied from initial 8dto final 1d.During the whole of enrichment and cultivation,the accumulation of ClO-3and ClO-2was not monitored and the perchlorate was reduced to Cl-.Successional change of microbial community was found with the time increasing of enrichment and cultivation by analyzing PCR-DGGE and similarity coefficient.It approved that microbial ecology could screen the survival of the fittest rapidly and adjust microbial community structure for adapting the environment.
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基本信息:
DOI：10.16441/j.cnki.hdxb.2013.11.012
中图分类号:X172
引用信息:
[1]高孟春,李亚惠,张优,等.氢自养高氯酸盐还原菌培养驯化的研究[J],2013,43(11):82-86.DOI:10.16441/j.cnki.hdxb.2013.11.012.
基金信息:
国家自然科学基金项目(21077096);; 中国海洋大学国家大学生创新训练项目(201210423090)资助