| 669 | 13 | 207 |
| 下载次数 | 被引频次 | 阅读次数 |
海洋浮游植物群落结构的改变与营养盐结构有关,不同形态氮可能会影响优势种生长从而改变浮游植物群落结构。本文针对莱州湾硅藻向甲藻潜在的种群演替问题,通过船基围隔生态系现场氮加富培养实验,研究NO3-N、NH4-N、陆源有机氮(DONts)和藻源有机氮(DONss)对海洋浮游植物群落结构的影响。结果表明,在NO3-N、NH4-N和DONts加富培养条件下,舟形藻(Navicula spp.,优势度60.4%)、丹麦细柱藻(Leptocylindrus danicusm,56.6%)和密连角毛藻(Chaetoceros densus,57.4%)等硅藻是优势种,在DONss加富培养条件下,春膝沟藻(Gonyaulax verior,60.2%)等甲藻为优势种,说明硅藻主要吸收无机态营养盐成为优势种,而甲藻能够吸收DON成为优势藻。动力学过程分析发现,春膝沟藻(G.verior)等甲藻可以直接吸收利用DONss,而DONts可能是通过矿化转化为无机氮,再被密连角毛藻(C.densus)等硅藻吸收与利用。本文研究成果,有助于对硅藻向甲藻演替的营养盐动力学控制机制的认识。
Abstract:The change of marine phytoplankton community structure is related to the structure of nutrients. Different forms of nitrogen may affect the growth of dominant species and also change the structure of phytoplankton community. In this paper, in view of the potential population succession of diatoms to dinoflagellates in Laizhou Bay, through the field culture experiment of ship-based enclosure ecosystem, the effects of NO3-N, NH4-N, organic nitrogen(DONts) from off-shore sources and marine autogenic organic nitrogen(DONss) on marine phytoplankton community structure were analizd. The results showed that, under the enrichment culture conditions of NO3-N, NH4-N and DONts, the dominant species were Navicula spp.(60.4%), Leptocylindrus danicus(56.6%) and Chaetoceros densus(57.4%). Under DONss enrichment culture conditions, dinoflagellates such as Gonyaulax verior(60.2%) were the dominant species. The reason is that diatoms mainly absorb inorganic nutrients and therefore become dominant species under NO3-N, NH4-N and DONts enrichment, and dinoflagellate can absorb DON and consequently become the dominant algae under DONss enrichment. Through dynamic process analysis, it was found that G. verior and other dinoflagellates can directly absorb and utilize DONss, while DONts may be converted to inorganic nitrogen through mineralization, and then be absorped and utilized by C. densus and other silicon algae. The research results in this paper can enhance the understanding for the dynamic nutrients control mechanism of succession from diatoms to dinoflagellates.
[1] 郭飞,刘森,王飞飞,等.夏季莱州湾水域营养盐现状及影响因素[J].海洋地质前沿,2016,32(2):38-44.Guo F,Liu S S,Wang F F,et al.Nutrient status and affecting factors of Laizhou Bay waters in summer[J].Marine Geology Frontiers,2016,32(2):38-44.
[2] 刘义豪,杨秀兰,靳洋,等.莱州湾海域营养盐现状及年际变化规律[J].渔业科学进展,2011,32(4):1-5.Liu Y H,Yang X L,Jin Y,et al.Nutrient status and inter-annual variation of Laizhou Bay waters[J].Progress In Fishery Sciences,2011,32(4):1-5.
[3] 李志林,石晓勇,张传松.春季黄渤海海水中尿素分布特征及溶解态氮的组成[J].环境科学,2015,36(11):3999-4004.Li Z L,Shi X Y,Zhang C S.Distribution characteristics of urea and composition of dissolved nitrogen in the Yellow Sea and Bohai Sea in spring[J].Environmental Science,2015,36(11):3999-4004.
[4] 王峰.基于海陆统筹的渤海入海污染物总量控制研究[D].青岛:中国海洋大学,2015.Wang F.Research on Total Amount Control of Pollutant Leaking into the Bohai Sea Based on Sea-Land Integrated Planning[D].Qingdao:Ocean University of China,2015.
[5] 张锦峰,高学鲁,李培苗,等.莱州湾西南部海域及其毗邻河流水体营养盐的分布特征及长期变化趋势[J].海洋通报,2015,34(2):222-232.Zhang J F,Gao X L,Li P M,et al.Distribution characteristics and long-term change trend of nutrients in the waters of southwestern Laizhou Bay and adjacent river[J].Marine Science Bulletin,2015,34(2):222-232.
[6] 郑军,郑东,张燕.加强小清河污染管理拯救莱州湾渔业资源[J].齐鲁渔业,2005(7):52.Zheng J,Zheng D,Zhang Y.Strengthen pollution management of Xiaoqing River and rescue fishery resources in Laizhou Bay[J].Shandong Fisheries,2005(7):52.
[7] 林国红,董月茹,李克强,等.赤潮发生关键控制要素识别研究—以渤海为例[J].中国海洋大学学报(自然科学版),2017,47(12):91-99.Lin G H,Dong Y R,Li K Q,et al.Study on identification of key control elements for red tide occurrence-taking Bohai Sea as an example[J].Periodical of Ocean University of China,2017,47(12):91-99.
[8] Thompson P A,Levasseur M E,Harrison P J J L,et al.Light-limited growth on ammonium vs.nitrate:What is the advantage for marine phytoplankton?[J].Limnology & Oceanography,1989,34(6):1014-1024.
[9] Li T-S,Yu R-C,Zhou M-J.Short-term effects of different nitrogen substrates on growth and toxin production of dinoflagellate Alexandrium catenella Balech (strain ACDH)[J].Harmful Algae,2011,12:46-54.
[10] Kamp A,Hogslund S,Risgaard-Petersen N,et al.Nitrate storage and dissimilatory nitrate reduction by eukaryotic microbes[J].Frontiers in Microbiology,2015,6(1492):1-15.
[11] Altman J C,Paerl H W.Composition of inorganic and organic nutrient sources influences phytoplankton community structure in the New River Estuary,North Carolina[J].Aquatic Ecology,2012,46(3):269-282.
[12] Malone T C,Falkowski P G,Hopkins T S,et al.Mesoscale response of diatom populations to a wind event in the plume of the Hudson River[J].Deep Sea Research Part a Oceanographic Research Papers,1983,30(2):149-170.
[13] Tzilkowski S S.Watershed Scale Controls on Urea Transport in a Coastal Plain River Network[D].[s.l.]:The Pennsywania State Vniversity,2012.
[14] Crandall J,Teece M A.Urea is a dynamic pool of bioavailable nitrogen in coral reefs[J].Coral Reefs,2012,31(1):207-214.
[15] Collos Y,Jauzein C,Ratmaya W,et al.Comparing diatom and Alexandrium catenella/tamarense blooms in Thau lagoon:Importance of dissolved organic nitrogen in seasonally N-limited systems[J].Harmful Algae,2014,37:84-91.
[16] Tonis P,Maestrini S Y,Maija B,et al.The role of inorganic and organic nutrients on the development of phytoplankton along a transect from the Daugava River mouth to the Open Baltic,in spring and summer 1999[J].Ices Journal of Marine Science,2003,60(4):827-835.
[17] Mccarthy J J,Taylor W R,Taft J L,et al.Nitrogenous nutrition of the plankton in the Chesapeake Bay.1.Nutrient availability and phytoplankton preferences[J].Limnology & Oceanography,1977,22(6):996-1011.
[18] Glibert P M,Garside C,Fuhrman J A,et al.Dependent coupling of inorganic and organic nitrogen uptake and regeneration in the plume of the Chesapeake Bay estuary and its regulation by large heterotrophs[J].Limnology and Oceanography,1991,36(5):895-909.
[19] Fan C,Glibert P M,Burkholder J A M.Characterization of the affinity for nitrogen,uptake kinetics,and environmental relationships for Prorocentrum minimum in natural blooms and laboratory cultures[J].Harmful Algae,2003,2(4):283-299.
[20] Wang X C,Litz L,Chen R F,et al.Release of dissolved organic matter during oxic and anoxic decomposition of salt marsh cordgrass[J].2007,105(3-4):309-321.
[21] Duce R A,LaRoche J,Altieri K,et al.Impacts of atmospheric anthropogenic nitrogen on the open ocean[J].Science,2008,320(5878):893-897.
[22] Cochlan W P,Herndon J,Kudela R M.Inorganic and organic nitrogen uptake by the toxigenic diatom Pseudonitzschia australis (Bacillariophyceae)[J].Harmful Algae,2008,8(1):111-118.
[23] Lonborg C,Sondergaard M.Microbial availability and degradation of dissolved organic carbon and nitrogen in two coastal areas[J].Estuarine Coastal & Shelf Science,2009,81(4):513-520.
[24] Gu X,Li K,Pang K,et al.Effects of pH on the growth and NH4-N uptake of Skeletonema costatum and Nitzschia closterium[J].Marine Pollution Bulletin,2017,124(2):946-952.
[25] Zhang L,Wang X,Han X,et al.Effects of petroleum hydrocarbon on the growth of marine algae:Experiment versus model[J].Journal of Ocean University of Qingdao,2002,32(5):804-810.
[26] Séférian R,Gehlen M,Bopp L,et al.Inconsistent strategies to spin up models in CMIP5:Implications for ocean biogeochemical model performance assessment[J].Geoentific Model Development Discussions,2015,8(10):8751-8808.
[27] Dueri S,Dahllof I,Hjorth M,et al.Modeling the combined effect of nutrients and pyrene on the plankton population:Validation using mesocosm experiment data and scenario analysis[J].Ecological Engineering,2009,220(17):2060-2067.
[28] Munoz-Tamayo R,Martinon P,Bougaran G,et al.Getting the most out of it:Optimal experiments for parameter estimation of microalgae growth models[J].Journal of Process Control,2014,24(6):991-1001.
[29] Mateus D M,Vaz M M,Pinho H J J E E.Fragmented limestone wastes as a constructed wetland substrate for phosphorus removal[J].Ecological Engineering,2012,41:65-69.
[30] Halpern B S,Walbridge S,Selkoe K A,et al.A global map of human impact on marine ecosystems[J].Science,2008,319(5865):948-952.
[31] 郭富,王保栋,辛明,等.2015年春、夏季莱州湾营养盐分布特征[J].海洋科学进展,2017,35(2):258-266.Gu F,Wang B D,Xin M,et al.Distribution characteristics of nutrients in Laizhou Bay in spring and summer of 2015[J].Advances in Marine Science,2017,35(2):258-266.
[32] 徐晓晨,苏莹,李克强,等.基于费效优化模型的小清河流域入海总氮污染物治理措施初步研究[J].中国海洋大学学报(自然科学版),2019,49(4):97-105.Xu X C,Su Y,Li K Q,et al.Preliminary study on treatment measures of total nitrogen pollutants into the sea of Xiaoqing River Basin based on cost-effective optimization model[J].Periodical of Ocean University of China,2019,49(4):97-105.
[33] 高懋芳.小清河流域农业面源氮素污染模拟研究[D].北京:中国农业科学院,2011.Gao M F.Simulation Study on Nitrogen Pollution from Agricultural Non-Point Sources in Xiaoqinghe River Basin[D].Beijing:Chinese Academy of Agriculture Sciences,2011.
[34] 毕聪聪.渤海环流季节变化及机制分析研究[D].青岛:中国海洋大学,2013.Bi C C.Analysis and Study on Seasonal Change and Mechanism of Circulation in Bohai Sea[D].Qingdao:Ocean University of China,2013.
[35] Duan K,Li K Q,Liang S K,et al.Optimizing a coastal monitoring network using a water-quality response grid (WRG)-based sampling design for improved reliability and efficiency[J].Marine Pollution Bulletin,2019,145:480-489.
[36] Lananan F,Jusoh A,Ali,Nora’aini,et al.Effect of Conway Medium and f/2 Medium on the growth of six genera of South China Sea marine microalgae[J].Bioresource Technology,2013,141:75-82.
[37] Utermohl H.Zur vervollkommnung der quantitativen phytoplankton-methodik:Mit 1 Tabelle und 15 abbildungen im Text und auf 1 Tafel[J].Internationale Vereinigung für theoretische und angewandte Limnologie:Mitteilungen,1958,9(1):1-38.
[38] Milliman J D,Qinchun X,Zwosheng Y J A J O S.Transfer of particulate organic carbon and nitrogen from the Yangtze River to the Ocean[J].America Journal of Science,1984,284(7):824-834.
[39] Jeffrey S,Humphrey G.New spectrophotometric equations for determining chlorophylls a,b,c1 and c2 in higher plants,algae and natural phytoplankton[J].Biochemie Und Physiologie Der Pflanzen,1975,167(2):191-194.
[40] Libiseller C,Grimvall A.Performance of partial Mann-Kendall tests for trend detection in the presence of covariate[J].Environmetrics,2010,13(1):71-84.
[41] Lu D L,Yun N,Liang S K,et al.Comparison of land-based sources with ambient estuarine concentrations of total dissolved nitrogen in Jiaozhou Bay (China)[J].Estuarine Coastal & Shelf ence,2016,180:82-90.
[42] Collos Y,Vaquer A,Souchu P.Does inhibition of nitrate uptake by ammonium lead to increased nitrogen uptake by phytoplankton?A test of the hypothesis with culture data and field data from the coastal ocean[C].[s.l.]:EGS-AGU-EUG Joint Assembly,2003,2572:2572.
[43] Pridmore R D,Rutherford J C.Modelling phytoplankton abundance in a small enclosed bay used for salmon farming[J].Aquaculture Research,1992,23(5):525-542.
[44] Ma L,Li J C A,Ahuja L R,et al.Root zone water quality model sensitivity analysis using monte carlo simulation[J].Transactions of the Asae,2000,43(4):883-895.
[45] Li K Q,Wang X L,Han X R,et al.Modelling nitrogen and phosphorus dynamics in a mesocosm pelagic ecosystem in Laizhou Bay in China[J].Journal of Ocean University of China,2009,8(2):133-140.
[46] 宋秀凯,程玲,付萍,等.莱州湾金城海域网采浮游植物年际变化及与环境因子的关系[J].生态学报,2019,39(8):144-153.Song X K,Cheng L,Fu P,et al.Interannual variation of phytoplankton harvesting in Jincheng sea area,Laizhou bay and its relationship with environmental factors[J].Acta Ecologica Sinica,2019,39(8):144-153.
[47] 张晓举,赵升.莱州湾西部浮游植物群落与环境因子关系的研究[J].大连海洋大学学报,2018,33(4):532-538.Zhang X J,Zhao S.Study on the relationship between phytoplankton community and environmental factors in the west of laizhou bay[J].Journal of Dalian Ocean University,2018,33(4):532-538.
[48] 袁骐,王云龙.N和P对东海中北部浮游植物的影响研究[J].海洋环境科学,2005,24(4):5-8.Yuan Q,Wang Y L.Study on the Effects of N and P on phytoplankton in the North Central China Sea[J].Marine Environmental Science,2005,24(4):5-8.
[49] Billen G,Somville M,Becker E D,et al.A nitrogen budget of the Scheldt hydrographical basin[J].Netherlands Journal of Sea Research,1988,19(3-4):223-230.
[50] Yun L,Li D,Tang J,et al.Long-term changes in the Changjiang Estuary plankton community related to anthropogenic eutrophication[J].Aquatic Ecosystem Health & Management,2010,13(1):66-72.
[51] Twomey L J,Piehler M F,Paerl H W.Phytoplankton uptake of ammonium,nitrate and urea in the Neuse River Estuary,NC,USA[J].Hydrobiologia,2005,533(1-3):123-134.
[52] Bronk D,See J,Bradley P,et al.DON as a source of bioavailable nitrogen for phytoplankton[J].Biogeosciences,2007,4(3):283-296.
[53] Bronk D,Ward B B.Inorganic and organic nitrogen cycling in the Southern California Bight[J].Biogeosciences,2005,52(12):2285-2300.
[54] Wen Z Y,Chen F.Optimization of nitrogen sources for heterotrophic production of eicosapentaenoic acid by the diatom Nitzschia laevis[J].Technology,2001,29(6-7):341-347.
[55] Glibert P M.Margalef revisited:A new phytoplankton mandala incorporating twelve dimensions,including nutritional physiology[J].Harmful Algae,2016,55(5):25-30.
[56] Andersen T,Schartau A,Paasche E.Quantifiying external and internal nitrogen and phosphorus pools,as well as nitrogen and phosphorus supplied through remineralization,in coastal marine plankton by means of a dilution technique[J].Mar Ecol Prog Ser,1991,69(69):67-80.
[57] Warns A,Hense I,Kremp A.Encystment of a cold-water dinoflagellate—From in vitro to in silico[J].Journal of Marine Systems,2013,125:54-60.
[58] áLvarez-Salgado X A,Castro C G,PéRez F F,et al.Nutrient mineralization patterns in shelf waters of the Western Iberian upwelling[J].Continental Shelf Research,1997,17(10):1247-1270.
[59] Berg G M,Glibert P M,JRgensen N O G,et al.Variability in inorganic and organic nitrogen uptake associated with riverine nutrient input in the Gulf of Riga,Baltic Sea[J].Estuaries,2001,24(2):204-214.
[60] Palenik B,Henson E,The use of amides and other organic nitrogen sources by the phytoplankton Emiliania huxleyi[J].Journal of Plankton Research,1997,42(7):1544-1551.
[61] Gettings R M,Townsend D W,Thomas M A,et al.Dynamics of late spring and summer phytoplankton communities on Georges Bank,with emphasis on diatoms,Alexandrium spp,and other dinoflagellates[J].Deep Sea Research Part II:Topical Studies in Oceanography,2014,103:120-138.
[62] Guicheng Z,Shengkang L,Xiao S,et al.Dissolved organic nitrogen bioavailability indicated by amino acids during a diatom to dinoflagellate bloom succession in the Changjiang River estuary and its adjacent shelf[J].Marine Chemistry,2015,176:83-95.
[63] Smayda T J,Reynolds C S.Community assembly in marine phytoplankton:application of recent models to harmful dinoflagellate blooms[J].Journal of Plankton Research,2001,5:5.
[64] Spilling K.Dense sub-ice bloom of dinoflagellates in the Baltic Sea,potentially limited by high pH[J].Journal of Plankton Research,2007,29(10):895-901.
基本信息:
中图分类号:Q948.8
引用信息:
[1]郜培怡,李克强,陈衎,等.氮形态组成对海洋浮游植物群落结构的影响与动力学研究[J],2021,51(05):57-71.
基金信息:
国家重点研究发展项目(2018YFC1407600);; 山东省重大科技创新工程项目(2019JZZY020705);; 中央高校基本科研业务费专项项目(201962008);; 国家自然科学基金项目(U1706215,41676062,NORC2018-01)资助~~
2020-05-03
2020
2020-07-01
2020
2020-05-28
1