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含酶防污涂层材料由于良好的环境友好性而受到广泛关注,蛋白酶是含酶防污涂层材料中的关键防污活性酶之一。本文基于海洋微生物产出蛋白酶对海洋环境具有较好的适应性和二氧化硅微球易于在防污涂层中作为填料应用的背景,选择牡蛎表面生物膜分离菌株所产蛋白酶作为防污活性酶,以正硅酸乙酯为原材料,采用反相乳液聚合法制备活性表面纳米二氧化硅微球(NPSiO2),通过3-氨丙基三乙氧基硅烷(APTES)对NPSiO2进行氨基化处理,并通过戊二醛将牡蛎源菌株产出蛋白酶共价固定于NPSiO2表面,制备出纳米二氧化硅微球共价固定化蛋白酶(NPSiO2-P)。相对于游离酶,固定化蛋白酶对温度、pH值的适应性明显提高,储存稳定性良好。以典型污损生物贻贝和底栖硅藻为评价生物,考察了固定化蛋白酶的防污性能。研究结果表明,固定化蛋白酶对硅藻、贻贝足丝的附着具有明显的抑制作用,同时可显著降低硅藻在材料表面的附着强度。
Abstract：Silica nanoparticles were prepared in nonionic water in an oil microemulsion and applied to immobilize protease produced by a Strain from Ostreaplicatula biofilm after amino modification and treatment of glutaraldehyde.Compared with the free enzyme,the adaptability of immobilized protease to temperature and pH was improved obviously,and with good storage stability.Antifouling performance of the immobilized protease was investigated by mussel byssus settlement and diatom attachment.The results showed that the immobilized protease not only significantly inhibited the adhesion of fouling organisms,but also weakened the adhesion strength of Navicula.Therefore,this work may be useful for the development of environmental-friendly antifouling surface.
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基本信息:
DOI：10.16441/j.cnki.hdxb.20170171
中图分类号:X172;X55
引用信息:
[1]王利,李跃瑞,于良民,等.牡蛎源菌株蛋白酶共价固定及其防污性能评价[J],2018,48(09):67-73.DOI:10.16441/j.cnki.hdxb.20170171.
基金信息:
国家重点基础研究发展计划项目(2014CB643305)资助~~
2017-04-13
2017
2017-06-28
2019-03-05
2019
2