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为了分离好氧反硝化细菌,探究其好氧反硝化过程。利用BTB培养基,从珍珠龙胆(♀Epinephelus fuscoguttatus×♂Epinephelus lanceolatu)循环水养殖系统的生物滤池中筛选出具有硝酸盐去除能力的细菌,并选择脱氮效果良好的菌株进行好氧反硝化反应器的构建,开展反硝化应用研究。本研究共分离出8株具有去除硝酸盐能力的菌株,经反硝化性能测定,都可大幅去除硝酸盐,同时也存在不同程度的氨氮和亚硝酸盐的积累;选择Z1、Z8两株脱氮效果较好的菌株进行好氧反硝化反应器的混合接种试验,结果显示反应器挂膜迅速、高效,接种2周后即达到相对稳定的水处理状态,硝酸盐去除率超过98.8%(约0.827g NO-3-N/(m2·d)),总氮去除率超过71.8%(约0.687g TN/(m2·d)),亚硝酸盐和氨氮的积累不明显,脱氮效果良好。
Abstract：In order to isolate aerobic bacteria and carry out some researches on aerobic denitrification,bromothymol blue(BTB)medium was used to isolated bacteria that can remove nitrate under aerobic condition from biofilter of marine recirculating aquaculture system(RAS).Isolated strains with well denitrifying ability would be selected and inoculated into the bioreactor together for setting up and studying aerobic denitrification bioreactor.Eight strains that could remove nitrate were isolated.According to the result of denitrification test,all the 8 strains can remove nitrate efficiently,and accumulate ammonia and nitrite at various degrees.Strain Z1 and Z8 with well denitrifing ability were selected for the aerobic denitrification bioreactor experiment.The result showed that the bioreactor reacted rapidly and effectively and reached a relatively stable status two weeks after inoculation.The nitrate removal rate was higher than98.8%(approximately 0.827 g NO-3-N/(m2·day))and the total nitrogen removal rate was higher than71.8%(approximately 0.687 g TN/(m2·day)).In contrast,nitrite and ammonia accumulated slightly.The denitrification bioreactor worked well.
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基本信息:
DOI：10.16441/j.cnki.hdxb.20170334
中图分类号:X172;X703
引用信息:
[1]陈钊,宋协法,黄志涛,等.循环水养殖系统中好氧反硝化细菌的分离和应用[J],2018,48(08):27-33.DOI:10.16441/j.cnki.hdxb.20170334.
基金信息:
国家科技支撑计划项目(2011BAD13B04);; 中国海洋大学青年教师科研专项基金项目(201413062);; 山东省博士后创新项目(201302025)资助~~