| 568 | 12 | 248 |
| 下载次数 | 被引频次 | 阅读次数 |
利用新型间歇增氧垂直流人工湿地,揭示了不同进水NH+_4-N浓度下盐度提升对湿地脱氮性能及微生物群落的影响。试验结果表明:间歇增氧垂直流人工湿地在盐胁迫下的脱氮性能明显优于传统垂直流人工湿地,高盐(2.0%盐度)对TN去除性能影响较大,对COD去除性能影响相对较小。进水NH+_4-N浓度为40 mg/L时,当盐度由0%提升至2.0%,出水NH+_4-N浓度由3.88 mg/L增至12.44 mg/L,NH+_4-N去除率由90.3%降至68.9%;出水NO~-2-N浓度在1.09～1.21 mg/L,波动较小,出水NO~-3-N浓度由5.19 mg/L降至3.81 mg/L;TN浓度由10.28 mg/L增至17.40 mg/L,TN去除率由74.3%降至56.5%;出水COD浓度由19.8 mg/L增至37.0 mg/L,COD去除率由89.5%降至81.5%。0.5%盐度下湿地微生物丰富度最高,且各盐度条件下曝气底部填料区微生物丰富度均高于湿地植物沙土区。随着湿地微生物对盐胁迫的逐步适应,微生物多样性初步恢复至无盐工况水平。盐度对人工湿地系统微生物菌群分布及其多样性产生显著影响,随着盐度从0.0%提升至2.0%,具有亚硝化功能的Nitrosomonas和具有硝化功能的Nitrospiraceae的丰度分别由2.5%、1.7%降至0.5%、0.3%,而具有反硝化功能的Thiobacillus和Denitratisoma平均丰度较高(分别为11.6%、5.4%)且保持相对稳定,表明其对盐胁迫具有较好的耐受性。此研究结果可为揭示盐度对人工湿地脱氮影响及推进间歇增氧垂直流人工湿地应用提供参考。
Abstract：The effect of salinity on nitrogen removal performance and microbial community of a novel patent intermittent aeration vertical flow constructed wetland(IA-VFCW) was investigated under different influent NH+_4-N concentrations. The results revealed that the nitrogen removal performance of IA-VFCW was obviously excellent than that of traditional vertical flow constructed wetland at different salinities. High salinity(2.0%) had a great influence on the TN removal efficiency, at the same time the impact on the COD removal efficiency was relatively unobvious. During the salinity was increased from 0% to 2.0%, the effluent NH+_4-N concentration was increased from 3.88 mg/L to 12.44 mg/L as the influent NH+_4-N concentration was 40 mg/L, and the NH+_4-N removal efficiency was sharply decreased from 90.3% to 68.9%, at the same time there was no NO~-2-N accumulation and the effluent NO~-2-N concentration was slightly fluctuated. The effluent TN concentration was increased from 10.28 mg/L to 17.40 mg/L, and the TN removal efficiency was gradually decreased from 74.3% to 56.5%. The effluent COD concentration was strongly stable with the increase of salinity to 1.0%. Furthermore, the effluent COD concentration was increased to 37.0 mg/L when the salinity further increased to 2.0%. Meanwhile, the COD removal efficiency was decreased from 89.5% to 81.5%. The microbial richness in IA-VFCW was the highest at 0.5% salinity. In addition, the microbial richness in the bottom of aeration area under different salinities was higher than that in the area of aquatic plants. As the microorganisms were gradually adapted to salt stress, the microbial diversity was closely restored to initial level in salt-free condition. The microbial distribution and diversity in IA-VFCW could be significantly affected by salinity. The abundance of Nitrosomonas and Nitrospiraceae was individually decreased from 2.5% to 0.5% and 1.7% to 0.3% with the increase of salinity, respectively. However, the abundance of Thiobacillus and Denitratisoma belonging to denitrification microorganism was kept at 11.6% and 5.4%, respectively, and remained relatively stable, indicating that they had better tolerance to salt stress. The research could reveal the effect of salinity on nitrogen removal performance and provide a guidance for practical engineering applications of IA-VFCW.
[1] 贾小宁，何小娟，韩凯旋，等.农村生活污水处理技术研究进展[J].水处理技术，2018,44(9):22-26.Jia X N,He X J,Han K X,et al.Research progress of sewage treatment technology in rural areas[J].Technology of Water Treatment,2018,44(9):22-26.
[2] 王玮，丁怡，王宇晖，等.人工湿地增氧技术在污水脱氮中的应用[J].工业水处理，2014,34(8):1-5.Wang W,Ding Y,Wang Y H,et al.Application of aeration technology to nitrogen removal in constructed wetlands[J].Industrial Water Treatment,2014,34(8):1-5.
[3] 翟俊，李岳.微曝气强化人工湿地处理生活污水试验研究[J].土木与环境工程学报，2020,42(6):178-184.Zhai J,Li Y.The effects of domestic wastewater treatment by micro-aerated hybrid constructed wetland[J].Journal of Civil and Environmental Engineering,2020,42(6):178-184.
[4] Foladori P,Ruaben J,Ortigara A R C.Recirculation or artificial aeration in vertical flow constructed wetlands:a comparative study for treating high load wastewater[J].Bioresource Technology,2013,149:398-405.
[5] Li J,Hu Z,Li F Z,et al.Effect of oxygen supply strategy on nitrogen removal of biochar-based vertical subsurface flow constructed wetland:Intermittent aeration and tidal flow[J].Chemosphere,2019,223(5):366-374.
[6] 亓鹏玉.间歇曝气人工水平潜流湿地脱氮除磷效果及氧化亚氮释放研究[D].济南：山东大学，2012.Qi P Y.Nitrogen and Phosphorus Removal and Nitrous Oxide Emissions from Intermittent Aeration Horizontal Subsurface Flow Constructed Wetlands[D].Jinan:Shandong University,2012.
[7] 汪健，李怀正，甄葆崇，等.间歇曝气对垂直潜流人工湿地脱氮效果的影响[J].环境科学，2016,37(3):980-987.Wang J,Li H Z,Zhen B C,et al.Effect of intermittent aeration on Nitrogen removal efficiency in vertical subsurface flow constructed wetland[J].Environmental Science,2016,37(3):980-987.
[8] 宋维星，周衍平，陈旭，等.基于响应曲面法的垂直流人工湿地脱氮工艺优化[J].中国海洋大学学报(自然科学版),2021,51(2):102-111.Song W X,Zhou Y P,Chen X,et al.Application of response surface methodology in optimizing aeration parameters of vertical flow constructed wetlands for nitrogen removal[J].Periodical of Ocean University of China,2021,51(2):102-111.
[9] 蔡舰，白承荣，巴图那生，等.盐度对芦苇(Phragmites australis)表流湿地除氮效果的影响[J].湖泊科学，2017,29(6):1350-1358.Cai J,Bai C R,Batonalon,et al.Effect of salinity on nitrogen removal performance in a Phragmites australis constructed surface flow wetland[J].Journal of Lake Sciences,2017,29(6):1350-1358.
[10] 王芬，段洪利，刘亚飞，等.人工湿地处理含盐富营养化水的植物根际与非根际菌群分析[J].环境工程学报，2020,14(7):1844-1851.Wang F,Duan H L,Liu Y F,et al.Analysis of bacterial community at the rhizosphere and non-rhizosphere of plants in constructed wetland treating brackish eutrophic water[J].Engineering,2020,14(7):1844-1851.
[11] 国家环境保护总局，水和废水监测分析方法编委会.水和废水监测分析方法[M].(第四版).北京：中国环境科学出版社，2002.Chinese NEPA.Water and Wastewater Monitoring Methods[M].Fourth ed.Beijing:Chinese Environmental Science Publishing Press,2002.
[12] 彭广生，陆燕青，曾鸿鹄，等.人工湿地β-六六六去除效果及细菌群落特征分析[J].水处理技术，2020,46(8):34-38.Peng G S,Lu Y Q,Zeng H H,et al.Purification effect of β-HCH by vertical flow constructed wetlands with different plant[J].Technology of Water Treatment,2020,46(8):34-38.
[13] 刘亚飞.垂直流人工湿地处理含盐富营养化水效果与脱氮菌群分析[D].天津：天津大学，2017.Liu Y F.Performance and Denitrifier Microbial Analysis for Vertical Flow Constructed Wetland Treating Eutrophic Brackish Water[D].Tianjin:Tianjin University,2017.
[14] 石雷，杨璇.人工湿地植物量及其对净化效果影响的研究[J].生态环境学报，2010,19(1):28-33.Shi L,Liu X.Phytomass and its impact on the pollutant removal ability of constructed wetland[J].Journal of Ecological Environment,2010,19(1):28-33.
[15] 王晓明，王杰.进水氨氮负荷对污水处理中硝化作用的影响[J].净水技术，2017,36(12):90-93.Wang X M,Wang J.Effect of different influent loading of ammonia nitrogen on nitrification in wastewater treatment[J].Water Purification Technology,2017,36(12):90-93.
[16] Liang Y X,Zhu H,Banuelos G,et al.Constructed wetlands for saline wastewater treatment:A review[J].Ecological Engineering,2017,98:275-285.
[17] 张千.基于固相反硝化和吸附除磷的低碳源污水脱氮除磷技术研究[D].重庆：重庆大学，2016.Zhang Q.Study on the Technology of Simultaneous Nitrogen and Phosporous Removal Based on Solid-Phase Denitrification and Phosphorous Adsorption[D].Chongqing:Chongqing University,2016.
[18] 陈李玉，李思潼，欧阳二明，等.盐度对活性污泥去除NH3-N及COD的影响研究[J].水处理技术，2019,45(5):82-84.Chen L Y,Li S T,Ouyang E M,et al.Study on effect of salinity for activated sludge on removal of NH3-N and COD[J].Technology of Water Treatment,2019,45(5):82-84.
[19] Panswad T,Anan C.Impact of high chloride wastewater on an anaerobic/anoxic/aerobic process with and without inoculation of chloride acclimated seeds[J].Water Research,1999,33(5):1165-1172.
[20] 郭姿璇，王群，佘宗莲.盐度对未驯化微生物活性的影响[J].中国环境科学，2017,37(1):181-187.Guo Z X,Wang Q,She Z L.Effects of salinity on the activity of non-acclimated biomass[J].China Environmental Science,2017,37(1):181-187.
[21] 王坤，柯水洲，袁辉洲，等.氨氮浓度对MBBR工艺中微生物群落结构的影响[J].环境工程，2020,38(9):119-125.Wang K,Ke S Z,Yuan H Z,et al.Effect of ammonia-nitrogen concentration on microbial community structure in a MBBR process[J].Environmental Engineering,2020,38(9):119-125.
[22] 王之敏，杨铭，王晓慧，等.盐度对SBR处理低碳氮比废水脱氮性能的影响[J].水处理技术，2019,45(9):120-125.Wang Z M,Yang M,Wang X H,et al.Effects of salinity on nitrogen removal performance of SBR for low C/N wastewater treatment[J].Water Treatment Technology,2019,45(9):120-125.
[23] 高锋，杨朝晖，李晨，等.人工湿地处理含盐生活污水的特性研究[J].环境科学，2012,33(11):3820-3825.Gao F,Yang Z H,Li C,et al.Treatment characteristics of saline domestic wastewater by constructed wetland[J].Environmental Science,2012,33(11):3820-3825.
[24] 王坤.进水氨氮浓度对MBBR工艺中微生物群落结构的影响研究[D].长沙：湖南大学，2019.Wang K.Effect of Influent Ammonia-Nitrogen Concentration on Microbial Community Structure in MBBR Process[D].Changsha:Hunan University,2019.
[25] 吕鹏翼.脱氮微生物的筛选及其在生物膜技术中的应用[D].北京：中国矿业大学(北京),2018.Lv P Y.Screening of Denitrogenation Microorganism and Its Application in the Biofilm Technique[D].Beijing:China University of Mining & Technology(Beijing),2018.
[26] Zhang K,Choi H,Dionysiou D D,et al.Application of membrane bioreactors in the preliminary treatment of early planetary base wastewater for long-duration space missions[J].Water Environment Research,2008,80(12):2209-2218.
[27] Wang T,Jiang Z,Dong W,et al.Growth and nitrogen removal characteristics of Halomonas sp.B01 under high salinity[J].Annals of Microbiology,2019,42(2):1-9.
[28] 徐金兰，陈紫薇，张少枫，等.信号分子联合脱氮硫杆菌对同步脱氮除硫的影响[J].环境科学，2019,40(9):4177-4184.Xu J L,Chen Z W,Zhang S F,et al.Effect of signal molecule combined with Thiobacillus denitrificans on simultaneous removal of nitrogen and sulfur[J].Environmental Science,2019,40(9):4177-4184.
[29] Li X,Yuan Y,Yuan Y,et al.Effects of salinity on the denitrification efficiency and community structure of a combined partial nitritation-anaerobic ammonium oxidation process[J].Bioresource Technology,2018,249:550-556.
[30] 侯爱月，李军，卞伟，等.不同短程硝化系统中微生物群落结构的对比分析[J].中国环境科学，2016,36(2):428-436.Hou A Y,Li J,Bian W,et al.Analysis of microbial community structure in different partial nitrification system[J].China Environmental Science,2016,36(2):428-436.
[31] 李炳荣，曹特特，王林，等.低氧条件下A2/O工艺对城市污水脱氮处理的中试研究[J].中国环境科学，2019,39(1):134-140.Li B R,Cao T T,Wang L,et al.A pilot-scale study on nitrogen removal of municipal wastewater by A2/O process under low dissolved oxygen condition[J].China Environmental Science,2019,39(1):134-140.
基本信息:
DOI：10.16441/j.cnki.hdxb.20210032
中图分类号:X703
引用信息:
[1]宋利国,宋维星,王宜东,等.盐胁迫对间歇增氧垂直流人工湿地脱氮性能及微生物群落影响[J],2022,52(03):106-116.DOI:10.16441/j.cnki.hdxb.20210032.
基金信息:
国家自然科学基金项目(51408570,51978636);; 中央高校基本科研业务费专项(201964005)资助~~
2021-01-23
2021
2021-04-02
2021
2021-03-25
1