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本文研究了10 mg/L的Ni2+和5 mg/L的金霉素(CTC)对序批式反应器(SBR)性能、微生物酶活性和微生物群落的影响。结果表明,COD去除率在反应器整个运行过程中始终保持在90%以上。10 mg/L Ni2+单独存在及其与5 mg/L CTC共存均能导致氮去除率的下降,且在二者共存时下降程度最大,表明Ni2+和CTC共存对氮的去除呈现协同抑制作用。随着进水中Ni2+和CTC的加入,活性污泥的耗氧速率、硝化和反硝化速率均有所降低。脱氢酶活性以及与脱氮相关的微生物酶活性的变化趋势与耗氧速率和相应的硝化反硝化速率的变化趋势保持一致,均出现了一定程度的降低。Ni2+和CTC共存对硝化反硝化活性呈现协同抑制作用,协同抑制作用主要依赖于Ni2+的含量。Ni2+和CTC共存对活性污泥中活性氧(ROS)的产生和乳酸脱氢酶(LDH)的释放呈现协同促进作用,可以诱导活性污泥细胞内氧化应激以及细胞损伤。长期暴露条件下添加10 mg/L Ni2+和5 mg/L CTC会降低微生物群落的丰富度和多样性。Ni2+和CTC的存在显著抑制了活性污泥中硝化菌(Nitrosomonas、Nitrospira)和反硝化菌(Thauera、Longilinea、Denitratisoma和Anaerolinea)的相对丰度,从而抑制了SBR的脱氮性能。
Abstract：The combined effects of Ni2+ at 10 mg/L and CTC at 5 mg/L on the performance, microbial enzymatic activity and microbial community in sequencing batch reactors(SBRs) were investigated. The COD removal efficiency was always above 90% during the whole operational process. The presence of 10 mg/L Ni2+ alone and its coexistence with 5 mg/L CTC caused the decrease of the nitrogen removal efficiencies, and their decreased degrees were the highest at the addition of mixed Ni2+ and CTC, indicating that the combined effect of Ni2+ and CTC had synergistic inhibitory effect on nitrogen removal. The special oxygen uptake rate, special nitrification and denitrification rates of activated sludge decreased with the addition of Ni2+ and CTC. The microbial enzymatic activities associated with the nitrogen removal showed similar change trends with the corresponding nitrification and denitrification rates with the addition of Ni2+ and CTC. The Ni2+ and CTC had synergistic inhibition effects on nitrifying and denitrifying activities. The combined toxicity was mainly dependent on Ni2+ level. The presence of Ni2+ and CTC promoted the activated sludge to produce more reactive oxygen species and release more lactate dehydrogenase, which could impact on the microbial morphology and physiological functions. The functional groups of sludge stressed by Ni2+ was altered more obvious than that by CTC. The richness and diversity of microbial community decreased with the addition of 10 mg/L Ni2+ and 5 mg/L CTC. The presence of Ni2+ and CTC significantly inhibited the relative abundance of nitrobacter(Nitrosomonas, Nitrospira) and denitrifiers(Thauera, Longilinea, Denitratisoma and Anaerolinea) in activated sludge and further inhibited the denitrification performance of SBR.
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基本信息:
DOI：10.16441/j.cnki.hdxb.20200367
中图分类号:X703;X172
引用信息:
[1]武原原,李姗姗,吴淑妍,等.Ni~(2+)和金霉素共存对序批式反应器性能、微生物活性及其微生物群落的影响[J],2021,51(10):89-100.DOI:10.16441/j.cnki.hdxb.20200367.
基金信息:
中央高校基本科研业务费专项(201964003)资助~~