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为探讨使用喹烯酮对刺参幼参的安全性,研究了喹烯酮对幼参生长、非特异性免疫及抗应激能力的影响。实验选用初始体重0.6g左右的幼参,采用单因素梯度设计,以不添加任何促生长剂的饲料作对照,在饲料中分别添加10、20、50、80和100mg/kg的喹烯酮,连续投喂69d,停药2周后经长途运输考察抗应激能力的变化。结果表明,添加喹烯酮的实验组的增重率、特定生长率和成活率都高于对照组,20mg/kg组最高,且显著高于其他组。适量的喹烯酮能提高刺参幼参体壁中SOD、AKP和ACP酶的活性,20mg/kg组最高;随着时间的延长,实验组与对照组的差异减小,50d后SOD、ACP活性接近对照组水平。高浓度喹烯酮(80~100mg/kg)使幼参的抗应激能力变差。研究表明,刺参苗种中间培育期间喹烯酮的最适用量为20mg/kg。
Abstract：To investigate the security of quinocetone on juvenile sea cucumber,the effect of quinocetone on the growth performance,non-specific immunity and anti-stress ability of juvenile sea cucumber were stu-died.The initial body weight of the animals was about 0.6g.Using a single factor gradient design,the experiment without adding growth promoter was used as the control.The animals were fed on feeds supplemented with 10,20,50,80 and 100mg/kg quinocetone for 69 days.The stress resistance after longdistance transportation was measured after acclimatization for two weeks.The results showed that the weight growth rate(WGR),specific growth rate(SGR)and survival rate(SR)were higher than those of control group when quinocetone was added into feed.When quinocetone was 20mg/kg,the WGR,SGR and SR were significantly higher than those of other groups.Quinocetone showed an effective immune enhancement as it improved the SOD,AKP and ACP enzyme activities in the body wall of animals.The maximal value were documented when quinocetone was 20mg/kg.The difference between treatments and control decreased with time.The enzyme activities of SOD and ACP in treatments generally decreased to that of control in 50 days.The experiments indicated that high concentration of quinocetone(80~100mg/kg)reduced the anti-stress ability of juvenile sea cucumber;thus 20mg/kg quinocetone was recommended during juvenile sea cucumber culture.
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基本信息:
DOI：10.16441/j.cnki.hdxb.20140023
中图分类号:S948
引用信息:
[1]安红红,张华威,宫向红,等.喹烯酮对刺参幼参生长、非特异性免疫及抗应激能力的影响[J],2015,45(03):67-72.DOI:10.16441/j.cnki.hdxb.20140023.
基金信息:
山东省科学技术发展计划项目(2012GHY11517);; 烟台市科技发展计划项目(2012134);; 国家海洋公益性行业科研专项项目(201105013);; 水生动物营养与饲料泰山学者岗位项目(2007-2012)资助