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本文考察了Cu2+冲击负荷对序批式反应器(SBR)脱氮性能、脱氮速率、微生物酶活性和微生物群落的影响,并评价了SBR的抗Cu2+冲击负荷能力。结果表明,在100 mg/L Cu2+冲击24 h后,COD的去除率由92.59%下降到47.01%,NH+_4-N的去除率由99.64%下降到45.76%。冲击停止后,SBR脱氮性能经过61天得到恢复。比耗氧速率、脱氮速率以及相关酶活性在Cu2+冲击60天后恢复正常水平。Cu2+冲击促进了活性氧的产生、乳酸脱氢酶的分泌,说明瞬时冲击引起了细胞氧化应激并使细胞膜受损。过氧化氢酶和超氧化物歧化酶的活性在瞬时Cu2+冲击下明显增加,以抑制活性氧的产生。微生物群落的丰富度和多样性在Cu2+负荷冲击下变化显著。SBR具有一定的抗瞬时Cu2+冲击负荷的能力,Cu2+冲击负荷消失后能够逐渐恢复其脱氮性能。
Abstract：The nitrogen removal performance, nitrogen removal rate, enzyme activity and microbial community of a sequencing batch reactor(SBR) were investigated under copper(Cu2+) shock loading stress, and resistance to the Cu2+ shock loading of SBR was evaluated. After adding the 100 mg/L Cu2+ shock loading for 24 hours, the removal efficiency of COD decreased from 92.59% to 47.01%, and the removal efficiency of NH+_4-N declined from 99.64% to 45.76%. The nitrogen removal performance of SBR recovered after 61 days without the existence of shock loading. The specific oxygen utilization rate(SOUR), nitrification rate, denitrification rate and the related enzyme activities returned to the pre-shock level after 60 days. The shock loading accelerated the production of reactive oxygen species(ROS), the release of lactate dehydrogenase(LDH), indicating that the instantaneous shock loading caused oxidative stress of cells and destroyed cell membranes. The activity of catalase(CAT) and superoxide dismutase(SOD) increased significantly under instantaneous Cu2+ shock loading to inhibit the production of ROS. The richness and diversity of microbial community changed significantly under Cu2+ shock loading. SBR has a certain ability to resist the shock loading of instantaneous Cu2+, and the nitrogen removal performance can be gradually restored after the instantaneous Cu2+ shock loading.
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基本信息:
中图分类号:X703
引用信息:
[1]潘云浩,孙艳霞,宋陈光,等.瞬时Cu~(2+)冲击负荷对序批式反应器脱氮性能、微生物活性和微生物群落的影响[J],2021,51(07):83-91.
基金信息:
中央高校基本科研业务费专项项目(201964003)资助~~