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为研究与生物膜相关的海洋细菌的多样性,在青岛近海海域,选取黏管藻、裙带菜、囊藻和浒苔4种大型海藻,分离其附生海洋细菌。使用2216E、海水R2A、TCBS和海水琼脂4种培养基平板,分离纯化得到99株海洋细菌。16SrD-NA序列分析表明,变形菌门细菌在数量、种类等方面,均占主导地位。这些菌株绝大多数为变形菌门的γ-变形菌纲(94株)和α-变形菌纲(3株),仅有1株为放线菌门,1株为厚壁菌门。其中丰度最高的3个属分别为假交替单胞菌属(Pseudoalteromonas,22株)、弧菌属(Vibrio,20株)和盐单胞菌属(Halomonas,15株),其次为食烷菌属(Alcanivorax,9株)、肠杆菌属(Enterobacter,8株)、假单胞菌属(Pseudomonas,7株)、气单胞菌属(Aeromonas,5株)、交替单胞菌属(Alteromonas,4株),其它9个属丰度较低。本研究为进一步探讨与生物膜相关的海洋细菌的多样性及其应用奠定了基础。
Abstract：In order to determine the bacterial diversity involving in biofilm formation,epiphytic bacteria were isolated from 4 macroalgae including Gloiosiphoniaelin Berkeley,Undaria pinnatifida,Colpomenia sinuosa and Enteromorpha prolifera inhabiting Qingdao coast where E.prolifera caused green tide appeared and endured from May to July,2008.In total,99 strains were isolated from these algae using four types of media including 2216E,marine R2A,TCBS and seawater agar.Proteobacteria dominant phylum in terms of quantity and the number of species as were revealed by 16S ribosomal RNA gene(rDNA) sequences alalysis;of them,most belonged to class Gammaproteobacteria.It was found that 94 strains belonged to this class;3 strains belonged to Alphaproteobacteria;1 strain belonged to Actinobacteria and 1 strain belonged to Firmicutes.The most abundant genus was Pseudoalteromonas(22 strains),followed by Vibrio(20 strains),Halomonas(15 strains),Alcanivorax(9 strains),Enterobacter(8 strains),Pseudomonas(7 strains),Aeromonas(5 strains) and Alteromonas(4 strains).Including these 8 genera,17 genera were identified in this study.Our findings indicated that the diversity of the epiphytic bacteria was high during green tide;however,the dominant strains and community structure were different from those found during normal period.
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基本信息:
DOI：10.16441/j.cnki.hdxb.2013.03.007
中图分类号:Q938.8
引用信息:
[1]赵锐,王红,史晓翀,等.青岛海区4种大型海藻可培养附生细菌多样性研究[J],2013,43(03):44-49.DOI:10.16441/j.cnki.hdxb.2013.03.007.
基金信息:
国家高技术研究发展计划项目(2007AA09Z434)资助