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研究鮸鱼鱼鳔多糖(Polysaccharide of swim bladder in Nibe Croaker,PSB)的单糖组成、分子量、理化性质及其对肝损伤的保护作用。以四氯化碳(CCl4)诱导急性肝损伤小鼠为模型对鱼鳔多糖进行保护肝脏方面研究,测定血清中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)和谷胱甘肽过氧化物酶(GSH-PX)的活力,并计算肝脏指数并测定肝脏中丙二醛(MDA)、总超氧化物歧化酶(SOD)、总一氧化氮合成酶(NOS)的含量,观察肝脏组织的病理切片。结果表明PSB为富含氨基糖和糖醛酸的硫酸化杂多糖,甘露糖、氨基葡萄糖、葡萄糖醛酸、氨基半乳糖、葡萄糖和半乳糖组成,分子量为89.7kDa。抗肝损伤的实验中与模型组相比,PSB的高剂量组显著地抑制ALT、AST和MDA的升高(P<0.01);PSB高剂量和中剂量组显著地提高GSH-PX活力,SOD和NOS的含量(P<0.01)。综上可见鮸鱼鱼鳔硫酸杂多糖PSB对四氯化碳诱导的小鼠急性肝损伤有一定的保护作用,这一作用可能与提高小鼠自身免疫有关。
Abstract：To explore the physicochemical property and hepatoprotective effect of the polysaccharides of swim bladder in Nibe Croaker(PSB)on carbon tetrachloride(CCl4)-induced acute liver injury in mice.The data showed PSB was a sulfated heteropolysaccharide mainly composed of galactosamine glucuronic acid and mannose with the molecular weight of 89.7 kDa.To study the hepatoprotective effect,the alanine aminotransferase(ALT),aspartate aminotransferase(AST)and glutathion peroxidase(GSH-PX)activities in the serum of the mice were determined.The liver index was calculated.The malondialdehyde(MDA),superoxide dismutase(SOD)and nitric oxide(NOS)contents in liver were also studied.Compared with the model group,feeding with high dose group of PSB revealed a significant decrease in the activity of ALT,AST and MDA(P<0.01).The high dose and middle dose group of PSB revealed a significant increase the content in GSH-PX,SOD and NOS(P<0.01).It showed that the sulfated heteropolysaccharides extracted from Swim Bladder in Nibe Croaker could protect CCl4-induced liver injury in rats,which may be related to enhance the ability of anti-oxidant activities of mice.
[1]朱元鼎,张春霖,成庆泰,等.东海鱼类志[M].北京:科学出版社,1963:308.Zhu Y D,Zhang C L,Cheng Q Q,et al.East Sea Fishes[M].Beijing:Science Press,1963:308.
[2]段振华,殷安齐,贾巧利,等.海鱼鱼鳔资源利用研究[J].食品研究与开发,2006(1):136-137.Duan Z H,Yin A Q,Jia Q L,et al.Utilizationtechnology of marine fish swim bladder[J]Food Research and Development,2006(1):136-137.
[3]段振华.鱼鳔加工应用的研究进展[J].中国食物与营养,2009(6):34-36.Duan Z H.Research progress of swim bladder processing applications[J].Food and Nutrition in China,2009(6):34-36.
[4]陈梅珍.鮸鱼鳔胶原蛋白的提取及其对小鼠学习记忆的影响[D].宁波:宁波大学,2011.Chen M Z.The Extraction of Swim Bladder of Miiuy Croaker Collagen And It's Effect on Learning and Memory In Mice[D].Ningbo:Ningbo University,2011.
[5]丁冬各,贺磊,于晶露,等.舟山鮸鱼资源的发展现状及其可持续发展对策[J].农村经济与科技,2016(7):71-73.Ding D G,He L,Gan J L.et al.Development status of the catfish resources in zhoushan and measures for its sustainable development[J].Rural Economy and Science-Technology,2016(7):71-73.
[6]韩慧敏,谢安,罗杰英.海参多糖提取工艺研究[J].湖南中医杂志,2000,16(1):56-57.Han H M,Xie A,Luo J Y.Study on the polysaccharide extraction technology from sea cucumber[J].Hunan Journal of Traditional Chinese Medicine,2000,16(1):56-57.
[7]董兰芳,张琴,许明珠.方格星虫多糖的分离纯化及单糖组成[J].食品科学,2015(1):109-112.1Dong L F,Zhang Q,Xu M Z,Isolation,Purification and monosaccharide analysis of polysaccharide from sipunculus nudus[J].Food Science,2015(1):109-112.
[8]戴军,朱松,汤坚,等.PMP柱前衍生高效液相色谱法分析杜氏盐藻多糖的单糖组成[J].分析测试学报,2007,26(2):206-210.Dai J,Zhu S,Tang J,et al.Analysis of monosaccharide compositions in polysaccharides from d.salina by pre-co lumn derivatization high performance liquid chromatography[J].Journal of Instrumental Analysis,2007,26(2):206-210.
[9]陈娜,尚宇,邱杨,等.肉制品中总糖含量测定方法的探讨[J].现代食品科技,2012(6):720-721.Chen N,Shang Y,Qiu Y,et al.Comparative study of the methods for determination of total sugar in meat products[J].Modern Food Science&Technology,2012(6):720-721.
[10]陈乾,马天翔,郭宏举,等.硫酸钡-比浊法测定褐藻糖胶中硫酸根的含量[J].药学实践杂志,2012(2):118-120.Chen Q,Ma T X,Guo H J,et al.Determination of the sulfate content in sulfated fucans by barium sulfate turbidity[J].Journal of Pharmaceutical Practice,2012(2):118-120
[11]殷秀红,赵峡,张紫恒,等.紫贻贝多糖的提取、分离和基本理化性质分析[J].中国海洋药物,2011(2):12-18.Yin X H,Zhao X,Zhang Z H,et al.Extraction,purification and physicochemical properties analyses of Myt ilus edulis Linnaeus polysaccharides[J].Chinese Journal of Marine Drugs,2011(2):12-18
[12]贺莉萍,郭毅,蒙艳斌,等.固定剂和固定时间对石蜡包埋组织RT-PCR检测病毒RNA的影响[J].国外医学:流行病学传染病学分册,2005(1):11-15.He L P,Guo Y,Meng Y B,et al.Effect of fixative and fixed_time on the detectability of viral RNA in paraffin_embedded tissue using RT-PCR[J].Foreign Medical Sciences Epidemiology Lemology,2005(1):11-15.
[13]Song Y H,Liu Q,Lv Z P,et al.Protection of a polysaccharide from Salvia miltiorrhiza,a Chinese medicinal herb,against immunological liver injury in mice[J].International Journal of Biological Macromolecules,2008,43(2):170-170.
[14]唐利波,龙尧,何松美.联苯双酯临床应用的再评价[J].实用肝脏病杂志,2006(3):185-188.Tang L B,Long Y,Heng S M,et al.Reevaluation of bifendate in clinical application[J].Journal of Practical Hepatology,2006(3):185-188.
[15]Yang J,Li Y,Wang F,et al.Hepatoprotective effects of apple polyphenols on CCl4-induced acute liver damage in mice[J].Journal of Agricultural and Food Chemistry,2010,58(10):6525-6531.
[16]Weber L W D,Boam M,Stampfl.Hepatotoxic“y and mechanism of action of halOalkanes:carbon tetrachloride as a toxicological mode[J].lCRC Critical Reviews in Toxicology,2003,33(2):105-136.
[17]Luckey S W,Petersen D R.Activation of Kupffer cells during the course of carbon tetrachloride-induced liver iniury and fibrosis in rats[J].Experimental and Molecular Pathology,2001,71(3):226-240.
[18]Kuriakose G C,Kurup M G.Antioxidant and antihepatotoxic effect of Spirulina laxissima against carbon tetrachloride induced hepatotoxicity in rats[J].Food&Function,2011,2(3-4):190-196.
[19]Jayakimar T,Ramesh E,Geraldine P.Antioxidant activity of the Oyster mushroom,Pleurotus ostreatus,on CCl4induced liver injury in rats[J].Food and Chemical Toxicology,2006,44(12):1989-1996.
[20]Saeed N,Khan M R,Shabbir M.Antioxidant activity,total-phenolic and total flavonoid contents of whole plant extracts Torilis leptophellal[J].BMC Complement Altern Med,2012,16(12):221-228.
[21]Mishra A,Paul S,Swarnakar S.Down regulation of matrix metal loproteinase-9by melatonin during prevention of alcohol-induced liver injury in mice[J].Biochimie,2011,93(5):854-856.
[22]王会,郭立,谢文磊.抗氧化剂抗氧化活性的测定方法(一)[J].食品与发酵工业,2006(3):92-98.Wang H,Guo L,Xie W L,Methods for determining antioxitive activity of antioxidants(Ⅰ)[J].Food ang Fermentation Industries,2006(3):92-98.
[23]Naik S R.Antioxidants and their role in biological functions:an overview[J].Indian Drugs,2003,40(9):501-516.
[24]Song F,Xie M L,Zhu L J,et al.Experimental study of osthole on treatment of hyperlipidemic and alcoholic fatty liver in animals[J].World Journal of Gastroenterology,2006(27):4359-4363.
[25]贺菊萍,潘迎捷,赵勇.牛蒡提取物对四氯化碳诱导肝损伤小鼠的保护作用[J].现代食品科技,2014(11):6-11.He J P,Pan Y J,Zhao Y.Hepatoprotective Effect of burdock extracts against carbon tetrachloride-induced liver damage in mice[J].Modern Food Science and Technology,2014(11):6-11.
[26]Minin E A,Buchwalow I B,Wellner M,et al.L-Arginine-NOcGMP signaling following acute liver injury in the rat[J].Experimental and Toxicology Pathology,2005,57(2):161-171.
[27]Hassen B A,Donia T,Laboratory of Human Physiology,et al.How to protect liver graft with nitric oxide[J].World Journal of Gastroenterology,2011(24):2879-2889.
[28]曾洋洋,韩章润,杨玫婷,等.海洋糖类药物研究进展[J].中国海洋药物,2013(2):67-75.Zeng Y Y,Han Z R,Yang G T,et al.An overview of marine polysaccharide-derived drugs in China[J].Chinese Journal of Marine Drugs,2013(2):67-75.
[29]牛庆凤,王斌,李涛,等.一种新型乌贼墨糖胺聚糖的分离纯化、理化性质及其抗氧化活性研究[J].中国海洋药物,2015,34(3):59-64.Niu Q F,Wang B,Li T,et al.Separation,purification and analyses on physicochemical properties,antioxideative activity of a novel glycosaminoglycans from Sepiaesculenta ink[J].Chinese Journal of Marine Drugs,2015,34(3):59-64.
[30]陈丽萍,王弘.硫酸多糖的结构与生物活性关系研究现状[J].广州化工,2005(5):21-23.Chen L P,Wang H.Research on relationship of chemical structure and bioactivity about sullfate polysaccharides[J].Guangzhou Chemical Industry,2005,33(5):21-23.
基本信息:
DOI：10.16441/j.cnki.hdxb.20170089
中图分类号:R285.5
引用信息:
[1]葛雪筠,周德健,王斌,等.鮸鱼鱼鳔多糖对四氯化碳引起的急性肝损伤的保护作用[J],2018,48(09):74-79+139.DOI:10.16441/j.cnki.hdxb.20170089.
基金信息:
国家自然科学基金项目(41406142);; 浙江省自然科学基金项目(21136000114)资助~~
2017-03-01
2017
2019-03-05
2019
2017-10-08
3