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为了探讨绿藻微观繁殖体在江苏紫菜养殖区的分布及其种类组成,分析其在绿潮形成发展过程中的作用,于2012年秋季对江苏近岸紫菜养殖区及附近海域进行水体和养殖区内沉积物、绠绳和养殖竹片的绿藻微观繁殖体调查研究。通过实验室培养萌发实验的方法确定了繁殖体在水体、沉积物、绠绳和养殖竹片上的数量,并对培养出的绿藻幼苗进行RFLP分析,确定其属种。结果表明,水体中微观繁殖体数量介于12~648株/L,高值区主要集中在紫菜养殖区站位,其中最高值出现在泥螺沙(NLS)站位。并且,小洋口(XYK)、高泥(GN)、泥螺沙(NLS)3个站位的水体、沉积物、绠绳和养殖竹片上均有绿藻繁殖体固着,其平均值分别为374株/L、7.9株/g、3.6和12.8株/cm2。通过分子检测发现,通过繁殖体培养萌发的绿藻种类主要存在浒苔(Ulva prolifera),缘管浒苔(Ulva linza)和Ulva sp.1 3种,其所占的比例分别是46%、14%和40%。因此,调查海域广泛存在绿藻微观繁殖体,其分布趋势呈现近岸高外海低,由养殖区向外扩散的趋势。江苏南部紫菜养殖区是繁殖体存在的一个重要源头。通过研究繁殖体在苏北浅滩的分布现状及种类组成情况,为该区域的绿潮溯源研究提供有力的支持。
Abstract：In order to investigate the distribution and species composition of micro-propagules in Porphyra yezoensis farming areas in southern Jiangsu and analysis of its role in the formation and development of green tides,we investigated micro-propagules of green algae in water,sediments,ropes and bamboos in seaweed farming areas of Jiangsu offshore in autumn,2012.The number of germination of micropropagules in the water,sediments,ropes and bamboos could be defined by laboratory culture experiments.Besides,the species of micro-propagules was determined by RFLP analysis.The research showed that there were large quantity of micro-propagules in water,which number ranged from 12 to 648ind/L.The high value was focused on seaweed farming area,which was the highest in the NLS station.In addition,there were micro-propagules in water,sediments,ropes and bamboos at XYK、GN and NLS stations,which average were 374ind/L,7.9ind/g,3.6and 12.8ind/cm2.By molecular testing,there were three kinds of green algal propagules such as Ulva prolifera,Ulva linzaand Ulva sp.1,which proportion were 46%,14% and 40%.Therefore,the distribution of micro-propagules in water was high value in shore and low value off shore.Besides,the trend was outward diffusion from the seaweed farming areas.It was an important source of micro-propagules in seaweed farming areas in southern Jiangsu.The study of distribution and species of micro-propagules in Jiangsu offshore provided support for researches of green tides in future.
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基本信息:
DOI：10.16441/j.cnki.hdxb.20140185
中图分类号:S968.4
引用信息:
[1]刘湘庆,王宗灵,张学雷,等.2012年秋季江苏南部紫菜养殖区绿藻微观繁殖体现状[J],2015,45(07):58-63.DOI:10.16441/j.cnki.hdxb.20140185.
基金信息:
国家自然科学基金项目(41276119);; 山东海洋科学研究中心联合基金项目(U1406403)资助