| 730 | 14 | 250 |
| 下载次数 | 被引频次 | 阅读次数 |
为研究氨氮急性胁迫对斑石鲷(Oplegnathus punctatus)幼鱼行为模式和血液学参数的影响，本研究设置了6、12和18 mg/L 3个不同的氨氮胁迫浓度组，以氨氮浓度0 mg/L作为对照组，分别在氨氮胁迫前和胁迫后对各处理组斑石鲷幼鱼的行为进行拍摄记录，并在研究结束前进行鱼体血液的采集和测定。研究表明，各氨氮胁迫组斑石鲷幼鱼的平均游泳速度和游泳轨迹的平均曲率均显著负相关(P<0.05),氨氮胁迫显著改变了斑石鲷幼鱼的行为模式，平均游泳速度下降和平均曲率上升是氨氮胁迫组区别于对照组的主要行为模式特征。不同胁迫浓度下斑石鲷幼鱼的行为不同，在氨氮浓度为6 mg/L时，斑石鲷幼鱼能通过机体调节逐渐适应不良环境，但当氨氮浓度达到12和18 mg/L时斑石鲷幼鱼的行为则受到不同程度抑制。急性氨氮胁迫导致斑石鲷幼鱼的血液学参数发生明显改变，其中18 mg/L氨氮组的斑石鲷幼鱼血液中白细胞数、血红蛋白浓度和红细胞数均显著低于对照组(P<0.05),而乳酸脱氢酶和谷丙转氨酶活性则均显著高于对照组(P<0.05);各胁迫组甘油三酯、尿酸浓度和碱性磷酸酶活性均显著高于对照组(P<0.05)。相关分析表明，氨氮胁迫8 h后斑石鲷幼鱼的平均游泳速度、运动时间百分比和平均曲率均与多种血液学参数显著相关。研究结果表明，氨氮急性胁迫可导致斑石鲷幼鱼行为异常和血液学参数显著改变，研究推测游泳速度下降和曲率增加是斑石鲷幼鱼对氨氮胁迫响应的典型行为特征。
Abstract：In this study, we investigated the effects of acute ammonia nitrogen stress on the behavior pattern and hematological parameters of juvenile spotted knifejaw(Oplegnathus punctatus). Three different ammonia nitrogen stress concentrations(0, 6, 12 and 18 mg/L) were set with 0 mg/L as the control. The behavior of juvenile spotted knifejaw each treatments was recorded by video before and after ammonia nitrogen stressing, and the fish blood was collected and measured before the end of study. The results showed that there was a significant negative correlation between average swimming speed and average curvature of the swimming track of juvenile spotted knifejaw(P<0.05). Ammonia nitrogen stress significantly changed the behavior pattern of juvenile spotted knifejaw. The decrease of the average swimming speed and the increase of the average curvature were the main behavior pattern of ammonia nitrogen stressing groups different from those of control. Compared with different stressing concentrations, when the ammonia nitrogen concentration was 6 mg/L, the juvenile spotted knifejaw gradually adapted to the adverse environment through body regulation, but when the ammonia nitrogen concentration reached 12 and 18 mg/L, the behavior of juvenile spotted knifejaw was gradually inhibited to varying degrees. Acute ammonia nitrogen stress resulted in significant changes in hematological parameters of juvenile spotted knifejaw. Under 18 mg/L ammonia nitrogen stress, the number of leukocytes, hemoglobin concentration and red blood cell number in the blood of juvenile spotted knifejaw were significantly lower than those of the control(P<0.05), while the activities of lactate dehydroge-nase and alanine aminotransferase were significantly higher than those of the control(P<0.05). The concentration of triglyceride, uric acid and alkaline phosphatase activity each stressing groups were significantly higher than those of the control(P<0.05). Correlation analysis showed that the average swimming speed, percentage of exercise time and average curvature of juvenile spotted knifejaw after 8 hours of ammonia nitrogen stressing were significantly correlated with a variety of hematological para-meters. In conclusion, acute ammonia nitrogen stressing can lead to abnormal behavior and significant changes in hematological parameters of juvenile spotted knifejaw. The decrease of swimming speed and increase of curvature may be the typical behavioral characteristics of juvenile O. punctatus in response to ammonia nitrogen stress.
[1] Gao X Q,Fei F,Huang B,et al.Alterations in hematological and biochemical parameters,oxidative stress,and immune response in Takifugu rubripes under acute ammonia exposure[J].Comparative Biochemistry and Physiology Part C:Toxicology & Pharmacology,2021,243:108978.
[2] Jia R,Liu B L,Han C,et al.Effects of ammonia exposure on stress and immune response in juvenile turbot (Scophthalmus maximus)[J].Aquaculture Research,2017,48(6):3149-3162.
[3] Shingles A,McKenzie D J,Taylor E W,et al.Effects of sublethal ammonia exposure on swimming performance in rainbow trout (Oncorhynchus mykiss)[J].Journal of Experimental Biology,2001,204(15):2691-2698.
[4] Aliko V,Mehmeti E,Qirjo M,et al.'Drink and sleep like a fish':Goldfish as a behavior model to study pharmaceutical effects in freshwater ecosystems[J].Journal of Biological Research-Bollettino della Società Italiana di Biologia Sperimentale,2019,92(1):1.
[5] Hamilton T J,Krook J,Szaszkiewicz J,et al.Shoaling,boldness,anxiety-like behavior and locomotion in zebrafish (Danio rerio) are altered by acute benzo[a]pyrene exposure[J].Science of The Total Environment,2021,774:145702.
[6] 靖锦杰，黄应平，袁喜，等.基于 MATLAB 轨迹跟踪研究重金属铜对鲢幼鱼自发游泳行为的影响[J].水生生物学报，2018,42(1):148-154.Jing J J,Huang Y P,Yuan X,et al.The effect of copper on the swimming ability of juvenils sliver carp based on MATLAB trajectory tracking[J].Acta Hydrobiologica Sinica,2018,42(1):148-154.
[7] Yalsuyi A M,Hedayati A,Vajargah M F,et al.Examining the toxicity of cadmium chloride in common carp (Cyprinus carpio) and goldfish (Carassius auratus)[J].Journal of Environmental Treatment Techniques,2017,5(2):83-86.
[8] Kim J H,Choi H,Sung G H,et al.Toxic effects on hematological parameters and oxidative stress in juvenile olive flounder,Paralichthys olivaceus exposed to waterborne zinc[J].Aquaculture Reports,2019,15:100225.
[9] Kim J H,Cho J H,Kim S R,et al.Toxic effects of waterborne ammonia exposure on hematological parameters,oxidative stress and stress indicators of juvenile hybrid grouper,Epinephelus lanceolatus♂× Epinephelus fuscoguttatus♀[J].Environmental Toxicology and Pharmacology,2020,80:103453.
[10] Fazio F,Marafioti S,Arfuso F,et al.Influence of different salinity on haematological and biochemical parameters of the widely cultured mullet,Mugil cephalus[J].Marine and Freshwater Behaviour and Physiology,2013,46(4):211-218.
[11] 赵玉柱，陈张帆，马腾，等.斑石鲷性腺发育的组织学观察与初步分析[J].上海海洋大学学报，2021,30(4):675-683.Zhao Y Z,Chen Z F,Ma T,et al.Histological observation and preliminary analysis of gonad development in spotted knifejaw (Oplegnathus punctatus)[J].Journal of Shanghai Ocean University,2021,30(4):675-683.
[12] 王雨福.斑石鲷 (Oplegnathus punctatus) 早期生长特征及消化系统发育的研究[D].北京：中国科学院大学，2015.Wang Y F.Studies on Early Growth and Development of Digestive System in Spotted Knifejaw (Oplegnathus punctatus)[D].Beijing:University of Chinese Academy of Sciences,2015.
[13] 王雨福，肖志忠，刘清华，等.斑石鲷早期发育的异速生长模式[J].海洋科学，2016,40(5):43-48.Wang Y F,Xiao Z Z,Liu Q H,et al.Allometric growth pattern during early ontogeny of spotted knifejaw (Oplegnathus punctatus)[J].Marine Sciences,2016,40(5):43-48.
[14] 钟鸿干，张旭，张海滨，等.南方地区斑石鲷工厂化养殖试验[J].中国水产，2015(5):64-66.Zhong H G,Zhang X,Zhang H B,et al.Industrialized culture experiment of Oplegnathus punctatus in southern China[J].China Fisheries,2015(5):64-66.
[15] 王海波，史成银，谢国驷，等.真鲷虹彩病毒引起养殖斑石鲷大规模死亡的研究[J].渔业科学进展，2020,41(4):151-158.Wang H B,Shi C Y,Xie G S,et al.Red seabream iridovirus causing mass mortality in farmed spotted knifejaw,Oplegnathus punctatus[J].Progress in Fishery Sciences,2020,41(4):151-158.
[16] Romero-Ferrero F,Bergomi M G,Hinz R C,et al.Idtracker.ai:Tracking all individuals in small or large collectives of unmarked animals[J].Nature Methods,2019,16(2):179-182.
[17] 徐建瑜.基于计算机视觉的急性应激条件下尼罗罗非鱼的行为研究[D].杭州：浙江大学，2005.Xu J Y.Behavioral Responses of Tilapia (Oreochromis niloticus) to Acute Stress Monitored by Computer Vision[D].Hangzhou:Zhejiang University,2005.
[18] 唐中华.捕食压力对乌江流域不同宽鳍鱲种群的标准代谢、个性和集群的影响[D].重庆：重庆师范大学，2018.Tang Z H.Predator-Driven Intra-species Variation in Maintenance Metabolism,Personalities and Shoaling Behavior in Pale Chub (Zacco platypus)[D].Chongqing:Chongqing Normal University,2018.
[19] Audira G,Sampurna B P,Juniardi S,et al.Establishing simple image-based methods and a cost-effective instrument for toxicity assessment on circadian rhythm dysregulation in fish[J].Biology Open,2019,8(6):1-9.
[20] Yalsuyi A M,Hajimoradloo A,Ghorbani R,et al.Behavior evaluation of rainbow trout (Oncorhynchus mykiss) following temperature and ammonia alterations[J].Environmental Toxicology and Pharmacology,2021,86:103648.
[21] Jolles J W,Boogert N J,Sridhar V H,et al.Consistent individual differences drive collective behavior and group functioning of schooling fish[J].Current Biology,2017,27(18):2862-2868.
[22] 程淑红，张仕军，李雷华，等.基于鱼群运动特征和 XGBoost 的异常水质监测[J].计量学报，2018,39(4):572-577.Cheng S H,Zhang S J,Li L H,et al.Water quality monitoring based on fish movement characteristics and XGBoost[J].Acta Metrologica Sinica,2018,39(4):572-577.
[23] Speedie N,Gerlai R.Alarm substance induced behavioral responses in zebrafish (Danio rerio)[J].Behavioural Brain Research,2008,188(1):168-177.
[24] Sarasamma S,Audira G,Juniardi S,et al.Evaluation of the effects of carbon 60 nanoparticle exposure to adult zebrafish:A behavioral and biochemical approach to elucidate the mechanism of toxicity[J].International Journal of Molecular Sciences,2018,19(12):3853.
[25] Hamilton T J,Tresguerres M,Kline D I.Dopamine D1 receptor activation leads to object recognition memory in a coral reef fish[J].Biology Letters,2017,13(7):20170183.
[26] Ho D H,Burggren W W.Blood-brain barrier function,cell viability,and gene expression of tight junction-associated proteins in the mouse are disrupted by crude oil,benzo[a]pyrene,and the dispersant COREXIT[J].Comparative Biochemistry and Physiology Part C:Toxicology & Pharmacology,2019,223:96-105.
[27] Fazio F,Saoca C,Ferrantelli V,et al.Relationship between arsenic accumulation in tissues and hematological parameters in mullet caught in Faro Lake:A preliminary study[J].Environmental Science and Pollution Research,2019,26(9):8821-8827.
[28] Kim J H,Kang Y J,Kim K I,et al.Toxic effects of nitrogenous compounds (ammonia,nitrite,and nitrate) on acute toxicity and antioxidant responses of juvenile olive flounder,Paralichthys olivaceus[J].Environmental Toxicology and Pharmacology,2019,67:73-78.
[29] 徐杨，肖炜，李大宇，等.慢性氨氮胁迫对尼罗罗非鱼幼鱼生长及生理功能的影响[J].南方农业学报，2015,46(2):327-331.Xu Y,Xiao W,Li D Y,et al.Effects of chronic ammonia stress on growth and physiological function of juvenile Nile tilapia (Oreochromis niloticus)[J].Journal of Southern Agriculture,2015,46(2):327-331.
[30] 龙章强.黑鲷(Acanthopagrus schlegeli)幼鱼对氨氮胁迫的生理响应及其维生素C的营养需求研究[D].上海：华东师范大学，2008.Long Z Q.The Physiological Responses to Ammonia Stress and Vitamin C Requirement of Juvenile Black Seabream (Acanthopagrus schlegeli)[D].Shanghai:East China Normal University,2008.
[31] 尹飞，孙鹏，彭士明，等.亚硝态氮和氨态氮急性胁迫下曼氏无针乌贼幼体血液的生化指标[J].生态毒理学报，2011,6(3):289-295.Yin F,Sun P,Peng S M,et al.Biochemical parameters in juvenile sepiella maindroni induced by acute exposure to nitrite-N or ammonia-N[J].Asian Journal of Ecotoxicology,2011,6(3):289-295.
[32] 蒋琦辰，顾曙余，张文逸，等.氨氮急性胁迫及其毒后恢复对红螯光壳螯虾幼虾相关免疫和代谢指标的影响[J].水产学报，2013,37(7):1066-1072.Jiang Q C,Gu S Y,Zhang W Y,et al.Acute effects of ammonia exposure on selected immunological and metabolic parameters in juvenile red claw crayfish (Cherax quadricarinatus) and the post-exposure recovery[J].Journal of Fisheries of China,2013,37 (7):1066-1072.
[33] 谢雨欣，张木子，黎明，等.氨氮胁迫下饥饿和再投喂对黄颡鱼幼鱼生长性能、血液健康、抗氧化能力及免疫应答的影响[J].动物营养学报，2018,30(8):9.Xie Y X,Zhang M Z,Li M,et al.Effects of starvation and refeeding on growth performance,blood health,antioxidant capacity and immune response of juvenile yellow catfish under ammonia nitrogen stress[J].Chinese Journal of Animal Nutrition,2018,30(8):9.
[34] 孙金辉，潘霞，徐永健，等.铜或镉胁迫对海洋青鳉生理生化指标及产卵量的影响[J].水生生物学报，2020,44(1):9.Sun J H,Pan X,Xu Y J,et al.Effects of copper/cadmium stresses on physiological and biochemical indexes and fecundity of Oryzias melastigma[J].Acta Hydrobiologica Sinica,2020,44(1):9.
[35] 谢松，陈立伟.重金属镉对黄颡鱼过氧化氢酶和乳酸脱氢酶活性的影响[C]// 北方七省市区动物学学术研讨会会议论文集.北京：北京动物学会，2011.Xie S,Chen L W.Effects of heavy metal cadmium on catalase and lactate dehydrogenase activities of Pelteobagrus fulvidraco[C]// Proceedings of the Symposium on Zoology in Seven Northern Provinces.Beijing:Beijing Zoological Society,2011.
[36] 王贞杰，陈四清，曹栋正，等.急性氨氮胁迫对圆斑星鲽(Verasper variegatus)幼鱼鳃和肝组织结构及相关酶活性的影响[J].渔业科学进展，2017,38(2):11.Wang Z J,Chen S Q,Cao D Z,et al.Effects of acute ammonia nitrogen stress on histopathology of gill and liver and enzyme activities of juvenile Verasper variegatus[J].Progress in Fishery Sciences,2017,38(2):11.
[37] Silva M ,Costa F F B D ,Leme F P,et al.Biological responses of Neotropical freshwater fish Lophiosilurus alexandri exposed to ammonia and nitrite[J].Science of the Total Environment,2018,616-617(1):1566-1575.
[38] 彭军辉，陈丽英，程长洪，等.氨氮对拟穴青蟹的急性毒性及对其血清免疫相关酶活力的影响[J].渔业科学进展，2018,39(5):114-121.Peng J H,Chen L Y,Cheng C H,et al.Acute toxicity of ammonia nitrogen to scylla paramamosain and its influence on immune factors in serum[J].Progress in Fishery Sciences,2018,39(5):114-121.
[39] 刘晓，黄六一，刘长东，等.光照颜色对虹鳟行为反应、血浆皮质醇和生化指标的影响[J].水产学报，2021,45(5):740-747.Liu X,Huang L Y,Liu C D,et al.Effects of light colors on behavior response,plasma cortisol and biochemical indexes of rainbow trout Oncorhynchus mykiss[J].Journal of Fisheries of China,2021,45(5):740-747.
基本信息:
中图分类号:S917.4
引用信息:
[1]张盛坤,徐进虎,孙雪倩,等.氨氮急性胁迫对斑石鲷幼鱼行为模式和血液学参数的影响[J],2023,53(03):120-132.
基金信息:
国家重点研究发展计划项目(2017YFE0122100,2020YFD0900201)资助~~
2022-01-27
2022
2022-01-28
2022
1