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本研究在青岛金沙滩潮上带连续采集浅层地下水样品,运用高通量测序技术及荧光定量PCR技术,探讨潮汐作用对滨海浅层地下水中细菌群落结构的影响。结果表明,滨海浅层地下水中的细菌优势类群为变形菌,潮汐作用会引起地下水中细菌群落组成与丰度发生明显变化。通过对各细菌样本的聚类分析发现,涨潮前期的样品之间、退潮阶段的样品之间相似性较高,而高潮阶段样品与它们的差异性较大。NO~-2、SiO■对浅层地下水中的细菌群落结构具有显著影响(P<0.05)。受潮汐作用影响,浅层地下水环境中与氮循环相关的细菌群落相对丰度较高。本研究表明,潮汐作用对浅层地下水中细菌群落结构的影响不可忽视。本研究为滨海浅层地下水中微生物群落结构的研究提供了可靠依据。
Abstract：In this study,the shallow groundwater samples were collected continuously in the upper tidal zone of Qingdao Golden Beach.The high-throughput sequencing and fluorescent quantitative PCR were used to investigate the influence of tide on bacterial community structure in coastal shallow groundwater.The results showed that Proteobacteria were the dominant group of microorganisms in the groundwater,and the tide significantly changed the composition and abundance of bacterial community in the groundwater.It was found that there was high community similarity among samples in the early rising tide period and ones in the falling tide period by cluster analysis,while there was significant difference in the samples from the high tide period.The NO2~-and SiO32-had important influence on the bacterial community structure in shallow groundwater(P<0.05).Influenced by the tide,the bacteria associated with nitrogen cycle in shallow groundwater was relatively abundant.This study demonstrates that it cannot be negligible that the influence of tide on bacterial community structure in shallow groundwater.
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基本信息:
DOI：10.16441/j.cnki.hdxb.20200289
中图分类号:Q938
引用信息:
[1]张胜东,王勋功,甄毓.潮汐作用对滨海浅层地下水中细菌群落结构的影响[J],2021,51(12):89-96.DOI:10.16441/j.cnki.hdxb.20200289.
基金信息:
国家自然科学基金项目(U1906210);; 青岛海洋科学与技术试点国家实验室海洋生态与环境科学功能实验室青年人才培育项目(LMEES-YTSP-2018-02-06);; 中央高校基本科研业务项目(201962003)资助~~
2020-10-09
2020
2021-04-02
2021
2021-03-25
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