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在模拟生态环境条件下研究了铜(Cu2+)、镉(Cd2+)和锌(Zn2+)3种重金属离子对2种海洋饵料微藻(青岛大扁藻(Platymonas helgolandica)和等鞭金藻8701(Isochrysis galbana 8701))的急性毒性效应,分析其对种群增长的影响,并利用透射电镜对微藻亚显微结构的变化进行分析。在实验浓度范围内,2种微藻的种群增长对3种重金属离子的急性胁迫表现出类似的变化趋势,随着胁迫浓度的增加,微藻的相对增长率均呈现出不断下降的趋势;通过比较2种微藻的48hEC50和96h-EC50发现:3种重金属离子的毒性程度为Cu2+>Cd2+>Zn2+,等鞭金藻8701对重金属胁迫的敏感性高于青岛大扁藻。2种微藻的亚显微结构观察显示,二者的膜结构及细胞核对Cu2+胁迫并不太敏感;与对照组相比等鞭金藻8701的色素体及贮能物质受影响较严重,而青岛大扁藻的叶绿体及淀粉粒受影响较大。
Abstract：The acute toxic effect of 3heavy metal ions(Cu2+,Cd2+and Zn2+)on the growth and ultrastructure of two marine bait microalgae,Platymonas helgolandicaand Isochrysis galbana8701was evaluated under controlled laboratory condition.Our findings included(1)two microalgae were similar in population growth trend when being exposed to different concentrations of heavy metal ions;and(2)two microalgae were differentially sensitive to 3ions.The growth of 2microalgae was inhibited by the ions;their growth continuously decreased with the increase of ion concentration.As was indicated by 48h-EC50and 96h-EC50,I.galbana8701was more sensitive to heavy metal stress than P.helgolandica.The toxicity of Cu2+was the highest,which was followed by Cd2+and Zn2+in order.The ultrastructure observed under TEM showed that the membrane system and nucleus of I.galbana8701and P.helgolandica were not so sensitive to Cu2+stress;while chlormatophore and starch grains were sensitive to Cu2+stress.
[1]国家海洋局.2009年中国海洋环境质量公报[J].海洋开发与管理,2010(4):24-57.
[2]Haritonidis S,Malea P.Bioaccumulation of metals by the green algaUlva rigidafrom Thermaikos Gulf,Greece[J].Environmental Pollution,1999,89(3):319-327.
[3]Edding M,Tala F.Copper Transfer and Influence on a Marine Food Chain[J].Bulletin of Environmental Contamination and Toxicology,1996,57(4):617-624.
[4]赵艳芳,俞志明,宋秀贤,等.营养盐对长江口2种主要赤潮原因藻光合色素和光合作用影响的比较研究[J].环境科学,2009,30(3):700-706.
[5]施国新,杜开和,解凯彬,等.汞、镉污染对黑藻叶细胞伤害的超微结构研究[J].植物学报,2000,42(4):373-378.
[6]周启星,孔繁翔,朱琳.生态毒理学[M].北京:科学出版社,2004:306-348.
[7]周汝伦,孙爱淑.金藻8701的分离、培养和应用初报[J].海洋湖沼通报,1990,1:34-40.
[8]周宏,项斯端.重金属铜、锌、铅、镉对小形月牙藻生长及亚显微结构的影响[J].杭州大学学报(自然科学版),1998,25(2):85-92.
[9]Guillard R R L.Culture of phytoplankton for feeding marine invertebrates[M]//Smith W L,Chanley M H.Culture of Marine Animals.New York:Plenum Press,1975:26-60.
[10]Mark P M.Hormesis Defined[J].Ageing Res Rev,2008,7(1):1-7.
[11]Melchor G D.The role of phytoplankton cells on the control of heavy metal concentration in seawater[J].Mar Chem,1995,48:215-236.
[12]Baker A J M.Metal Tolerance[J].New Phytologist,1987,106:93-111.
[13]李英敏,杨海波,吕福荣,等.叉鞭金藻对微量锌镉的吸附效应研究[J].环境污染与防治,2004,26(5):396-398.
[14]Satoha A,Vudikariab L Q.Evaluation of the sensitivity of marine microalgal strainsto the heavy metals,Cu,As,Sb,Pb and Cd[J].Environment International,2005,31(5):713-722.
[15]刘春凤,吴雪,高悦.两种等鞭金藻外部形态与超微结构的观察比较[J].大连水产学院学报,2008,23(4):273-277.
[16]Garrido J,Gaspar A,Garrido E M.Alkyl esters of hydroxycinnamic acids with improved antioxidant activity and lipophilicity protect PC12cells against oxidative stress[J].Biochimie,2012,94:961-967.
[17]苏秀榕,刘照彬,迟庆宏.从超微结构变化研究Cu2+,Zn2+,Se4+,Cd2+对三角褐指藻的毒性效应[J].辽宁师范大学学报,2000,23(1):68-72.
[18]梁英,王帅.重金属对微藻胁迫的研究现状及前景[J].海洋湖沼通报,2009,11(4):72-82.
基本信息:
DOI：10.16441/j.cnki.hdxb.2014.02.008
中图分类号:S963
引用信息:
[1]于小娣,师玥,刘泳,等.重金属胁迫对两种海洋饵料微藻的急性毒性效应研究[J],2014,44(02):53-59.DOI:10.16441/j.cnki.hdxb.2014.02.008.
基金信息:
国家自然科学基金项目(41076066;31071458)资助