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2026, 04, v.56 27-38
胰岛素样促雄腺激素基因敲低对凡纳滨对虾生殖-代谢调控网络的影响
基金项目(Foundation): 国家自然科学基金青年科学基金项目(32202895); 山东省优秀青年科学基金项目(海外)(2023HWYQ-055); 中央高校基本科研业务费专项资金项目(202212022)资助~~
邮箱(Email): qz@ouc.edu.cn;
DOI: 10.16441/j.cnki.hdxb.20250142
摘要:

为深入探究凡纳滨对虾(Litopenaeus vannamei)胰岛素样促雄腺激素(Insulin-like androgenic gland hormone, IAG)对基因表达调控的影响,本研究通过体内IAG的敲低和重组蛋白处理进行验证。通过设置4个诱导温度:16、20、28和37℃,探究了温度对IAG重组蛋白原核表达效率的影响。研究显示,在原核表达系统中28℃诱导12 h, pET32a-IAG重组蛋白在包涵体中的产量相对较高。在IAG dsRNA短期干扰实验中,IAG的表达量显著降低了97%。同时,促雄腺转录组分析表明生殖相关基因CYP2L1以及花生四烯酸途径基因GPx的表达显著上调,而代谢相关基因SEC63、TnC的表达显著下调(P<0.05)。研究结果表明,IAG在凡纳滨对虾的生殖和生长发育过程中起到重要的调控作用,其表达水平的改变能够显著影响生殖和代谢相关基因的表达模式,进而影响生殖与生长发育的过程。

Abstract:

To deeply investigate the effect of insulin-like androgenic hormone(IAG) on the regulation of gene expression in shrimp Litopenaeus vannamei, we conducted in vivo IAG knockdown and recombinant protein treatment experiments. By setting four induction temperatures, 16, 20, 28 and 37 ℃, we investigated the influence of temperature on the prokaryotic expression efficiency of the IAG recombinant protein. The findings showed that induction at 28 ℃ for 12 hours resulted in a relatively high yield of pET32a-IAG recombinant protein in the inclusion bodies of the prokaryotic expression system. In the IAG dsRNA short-term interference experiment, IAG expression was significantly reduced by 97%. Meanwhile, transcriptome analysis of the androgenic gland showed a significant up-regulation of the expression of the reproduction-related gene CYP2L1, as well as the arachidonic acid pathway gene GPx, while the expression of the metabolism-related gene TnC was significantly down-regulated(P<0.05). The results of this study suggested that IAG may play an important regulatory role in the process of reproduction and development of L. vannamei, and that changes in its expression level can significantly affect the expression pattern of reproduction-related and metabolism-related genes, which in turn may influence the process of reproduction and development.

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基本信息:

DOI:10.16441/j.cnki.hdxb.20250142

中图分类号:S917.4

引用信息:

[1]杜莹,陈颖,孙小娟,等.胰岛素样促雄腺激素基因敲低对凡纳滨对虾生殖-代谢调控网络的影响[J].中国海洋大学学报(自然科学版),2026,56(04):27-38.DOI:10.16441/j.cnki.hdxb.20250142.

基金信息:

国家自然科学基金青年科学基金项目(32202895); 山东省优秀青年科学基金项目(海外)(2023HWYQ-055); 中央高校基本科研业务费专项资金项目(202212022)资助~~

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