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菲律宾蛤仔软体部经匀浆、乙醇提取、旋转蒸发浓缩,得到菲律宾蛤仔乙醇提取物,乙醇提取物经有机溶剂萃取,依次得到乙酸乙酯萃取物、石油醚萃取物和水溶性组分。在32℃,pH=6.8的Na2HPO4-NaH2PO4缓冲体系中,研究菲律宾蛤仔提取物对酪氨酸酶活力的抑制作用。结果表明,菲律宾蛤仔乙醇提取物对酪氨酸酶有明显的抑制作用,其IC50为0.388 mg/mL。进一步动力学分析表明,乙酸乙酯萃取物、石油醚萃取物对酪氨酸酶具有非竞争性可逆抑制作用,水溶性组分对酪氨酸酶具有竞争性可逆抑制作用。
Abstract：The soft tissue of Ruditapes philippinarum was separated to obtain the extract by pounding to pieces,marinating in ethanol and distilling to a concentrated liquid.The inhibitory capability of the extract from Ruditapes philippinarum against tyrosinase activity was studied with Na2HPO4-NaH2PO4 as buffer solution(pH=6.8) and at 32 ℃.The results showed that the ethanolic extract from Ruditapes philippinarum can inhibit the activitiy of tyrosinase obviously,the concentration corresponding to 50% inhibitory rate(IC50) is 0.388 mg/mL.The ethanolic extract was leached with ethyl acetate and petroleum ether.Lineweaver-Burk plots demonstrate that the inhibition effect of the ethyl acetate-soluble fraction and the petroleum ether-soluble fraction express as what a noncompetitive inhibitor does,the substance in water expresses as what a competitive inhibitor does.
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基本信息:
DOI：10.16441/j.cnki.hdxb.2009.06.014
中图分类号:S917.4
引用信息:
[1]焉翠蔚,徐睿,孙妍,等.菲律宾蛤仔提取物抑制酪氨酸酶活性的研究[J].中国海洋大学学报(自然科学版),2009,39(06):1233-1236+1260.DOI:10.16441/j.cnki.hdxb.2009.06.014.
基金信息:
国家自然科学基金项目(30672515)资助
2009-11-15
2009-11-15