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高效液相色谱-质谱联用(HPLC-MS)技术,将HPLC的高分离效能与MS的强大结构鉴定功能结合起来,已逐渐成为天然产物化学成分快速分离、鉴别的强有力手段。本研究采用高效液相色谱-电喷雾质谱联用(HPLC-ESI-MS)技术,成功建立了刺参皂苷快速鉴别方法,并对市售干刺参中的4种皂苷进行了鉴别。此外,应用该技术对罗氏海盘车中的13种化学成分进行了快速鉴别。为海洋药用生物化学成分快速鉴别提供了1种值得推广的新方法,为更好的开发利用海洋天然药物奠定了基础。
Abstract：In recent years,high performance liquid chromatography-mass spectrometry combined the high separation efficiency of HPLC with rapid identification function of MS had become a powerful tool for the rapid separation and identification of chemical components in natural products.In the present study,a high performance liquid chromatography-electrospray ionization mass spectrometry(HPLC-ESI-MS) method was developed for rapid identification of 4 saponin components in dry Holothurian sold on the market.On the other hand,13 compounds in Asterias rollestoni were also identified by HPLC-ESI-MS.The HPLC-MS method presented in this paper for the rapid identification of marine compounds was worth popularizing and to lay a foundation for the further exploration of marine natural products.
[1]Cai Z W,Lee F S C,Wang X R,et al.Acapsule review of recentstudies on the application of mass spectrometry in the analysis of Chi-nese medicinal herbs[J].J Mass Spectrom,2002,37:1013-1024.
[2]刘荣霞,果德安,叶敏,等.液质联用技术(LC-MS)在中药现代研究中的应用[J].世界科学技术-中药现代化,2005,7(5):33-40.
[3]Tolstikov V V,Fiehn O.Analysis of highly polar compounds ofplant origin:Combination of hydrophilic interaction chromatogra-phy and electrospray ion trap mass spectrometry[J].Anal Bio-chem,2002,301:298-307.
[4]王小如.中药及中药材质量控制关键技术[M].北京:化学工业出版社,2006.
[5]林文翰.我国海洋生物的药学研究思考[J].中国天然药物,2006,4(1):10-14.
[6]李国强,王斌,管华诗.海洋糖苷类成分生物活性研究进展[J].中国海洋大学学报:自然科学版,2007,37(4):541-549.
[7]易杨华,李玲,汤海峰.海洋药物研究进展[J].上海医药,2003,24(2):75-77.
[8]郭跃伟.海洋天然产物和海洋药物研究的历史、现状和未来[J].自然杂志,2009,31(1):27-32.
[9]王燕,乔善义.海洋天然产物的药物开发[J].国际药学研究杂志,2009,36(4):307-309.
[10]孙鹏,易杨华,李玲,等.海参皂苷的生源分类和化学结构特征(楯手目)[J].中国天然药物,2007,5(6):463-468.
[11]张虹,吴雪美.高效液相色谱-质谱联用法分析冬虫夏草及其功能食品中的活性成分[J].中国食品学报,2007,7(5):113-121.
[12]Li G Q,Deng Z W,Li J,et al.Chemical Constituents fromStar-fish Asterias rollestoni[J].Journal of Chinese PharmaceuticalSciences,2004,13(2):81-86.
基本信息:
DOI：10.16441/j.cnki.hdxb.2010.05.024
中图分类号:R284.1
引用信息:
[1]张道来,陈军辉,周明,等.高效液相色谱-质谱联用技术在海洋药用生物化学成分快速鉴别中的应用[J].中国海洋大学学报(自然科学版),2010,40(05):99-102.DOI:10.16441/j.cnki.hdxb.2010.05.024.
基金信息:
国家自然科学基金项目(20675021,20905017);; 中国近海海洋综合调查与评价908专项项目(908-02-05-04);; 海洋局青年基金资助
2010-05-15
2010-05-15