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针对生物医用材料在使用过程中易引发细菌感染的问题，本研究通过抗菌化处理硅藻生物硅来降低材料表面的细菌附着，从而降低潜在的伤口感染风险。本文通过还原法对硅藻生物硅进行原位负载银纳米颗粒(AgNPs)。以硝酸银为银源，以聚乙烯吡咯烷酮(PVP)为分散保护剂，制备抗菌化硅藻生物硅——载银硅藻生物硅(DB-AgNPs)。研究了不同还原剂和初始银离子添加量对DB-AgNPs负载AgNPs的影响。探讨了DB-AgNPs的Ag+释放能力、载银量及抗菌效果。研究表明，以硼氢化钠为还原剂，能够实现DB对AgNPs的高效负载，使得AgNPs的数量更多且分散性良好。DB-AgNPs的载银量与初始银离子添加量有关，随着初始银离子添加量的增加，DB-AgNPs的载银量相应升高。其中，初始银离子添加量为16.70 mg/mL时，DB-AgNPs3的载银量最高，为22.57%。银离子释放实验显示，DB-AgNPs表现出缓慢释放Ag+的能力。在抑菌实验中，DB-AgNPs对大肠杆菌(Escherichia coli)、金黄色葡萄球菌(Staphylococcus aureus)和变形链球菌(Streptococcus mutans)均表现出有效的抑菌能力(抑菌率均大于90%,且形成明显的抑菌圈)。研究结果表明，本文通过原位负载AgNPs的方法，制备了一种新型抗菌化硅藻生物硅——DB-AgNPs。DB-AgNPs具有较高的载银量，可通过释放Ag+达到高效、广泛的抗菌效果。本研究结果可进一步拓展硅藻生物硅在抗菌生物材料领域的应用。
Abstract：This study was carried out to reduce bacterial attachment to the surface of diatom biosilica through antibacterial treatment, and avoid the potential risk of wound infection. The reduction method of in situ loading AgNPs onto diatom biogenic silicon was used to prepare the antibacterial diatom biogenic silica DB-AgNPs. Silver nitrate was used as the silver source, and polyvinylpyrrolidone(PVP) as the dispersant protector. The effects of different reducing agents and initial silver ion addition on loading AgNPs onto DB-AgNPs were studied with the Ag+ release ability, silver loading capacity and antibacterial effect of DB-AgNPs explored. Sodium borohydride was used as a reducing agent to achieve efficient loading of DB-AgNPs onto AgNPs. The silver loading of DB-AgNPs was related to the initial amount of silver ions added. With the increase of initial amount of silver ions added, the silver loading of DB-AgNPs increased correspondingly. When the initial silver ion was 16.70 mg/mL, the silver loading of DB-AgNPs3 maximized at 22.57%. The Ag+ release experiment showed that DB-AgNPs exhibited the ability to slowly release Ag+. In the antibacterial experiment, DB-AgNPs showed effective antibacterial ability against E. coli, S. aureus and S. mutans with inhibition rate greater than 90%. A novel antibacterial diatom biosilicon DB-AgNPs was prepared by in situ loading AgNPs. DB-AgNPs have a high silver loading capacity, which showed efficient and extensive antibacterial effects by releasing Ag+. Its application can extend our trials of using diatomaceous biosilicon as the antibacterial biomaterials.
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基本信息:
DOI：10.16441/j.cnki.hdxb.20240102
中图分类号:TB34;R318.08
引用信息:
[1]刘金凤,孙小杰,车晟廷,等.以原位负载AgNPs的策略制备抗菌化硅藻生物硅的研究[J].中国海洋大学学报(自然科学版),2025,55(06):40-49.DOI:10.16441/j.cnki.hdxb.20240102.
基金信息:
国家自然科学基金项目(U22A20588); 三亚市创新专项(2022KJCX57); 崂山实验室专项(12-04); 海南省教育厅支持项目(Hnjg2024-276)资助~~
2025-05-26
2025-05-26