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利用实验生态学的方法,探讨盐度日节律性连续变化对孔石莼(Ulva pertusa)生长和生化组成的影响,旨在查明潮间带大型海藻对盐度日节律性连续变化的适应性。实验共设5个盐度日节律变化处理(30±3,30±6,30±9,30±12和30±15)和1个恒盐(30±0)对照处理。实验结果表明:孔石莼能够耐受较大范围的盐度日节律性连续变化,这种变化虽然对孔石莼的生长不利,但是仍然能够使孔石莼的相对生长率保持在12%以上;盐度变化幅度±3~±15与恒盐对照相比明显不利于孔石莼的生长,盐度变化幅度越大,孔石莼生长越缓慢。同恒盐处理相比,盐度日节律连续变化对孔石莼叶绿素含量的影响不明显,但叶绿素a和叶绿素b含量都有随着盐度变化幅度的增大而逐渐减少的趋势。盐度日节律性连续变化使孔石莼蛋白质和可溶性糖的相对含量显著增加(P<0.05),随着盐度变化幅度的增大,蛋白质和可溶性糖增加的趋势越明显,并且在盐度日节律性连续变化下,蛋白质增加的速度比可溶性糖增加的速度要快。同时,游离脯氨酸的相对含量随着盐度变化幅度的增加而增加,但是在缓和的盐度日节律性连续变化下(30±3)孔石莼游离脯氨酸的含量却显著小于恒盐对照组(P<0.05)。盐度日节律性连续变化同恒定盐度相比,虽不利于孔石莼的生长,却促进了孔石莼生化产物的合成,是海藻干露时品质得以提高的原因之一。
Abstract：The marcoalga Ulva pertusa was cultured under 30±3,30±6,30±9,30±12 and 30±15 circadian rhythms of fluctuating salinity conditions,and a constant salinity of 30 was used as the control.The growth rates of macroalga under fluctuating salinity conditions were significantly lower than that at the constant salinity of 30,while the RGR of U.pertusa under different fluctuating salinity conditions were all kept at least 12%.Analysis of biochemical composition at the final stages of thaulls growth revealed that diel fluctuating salinity caused various influences(P<0.05).The contents of chlorophyll a and chlorophyll b under fluctuating salinity conditions were slightly lower than those at constant salinity of 30,however no statistically significant differences were found among them(P>0.05).The contents of protein and total solute carbohydrate under fluctuating salinity conditions were significantly higher than those at constant salinity of 30(P<0.05),and there was a trend that the content of proline increased with the increase of amplitude of fluctuating salinity,while the content of proline during mild salinity of changes(30±3) was significantly lower than that at constant salinity(P<0.05). Therefore,more protein and carbohydrate production might account for the enhancement in the quality of macroalga at the diel flunctuating salinity in the present study.
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基本信息:
DOI：10.16441/j.cnki.hdxb.2007.06.010
中图分类号:S917.3
引用信息:
[1]王巧晗,董双林,田相利,等.盐度日节律性连续变化对孔石莼生长和生化组成的影响[J].中国海洋大学学报(自然科学版),2007,No.158(06):911-915.DOI:10.16441/j.cnki.hdxb.2007.06.010.
基金信息:
国家十一五支撑计划(2006BAD09A01);; 国家十一五863项目(2006AA10Z409)资助
2007-11-15
2007-11-15