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通过硅胶柱层析法分离得到多棘海盘车脑苷脂,并采用气相色谱质谱联用(GC-MS)及电喷雾质谱(ESI-MS)分析鉴定其结构。GC-MS分析表明,多棘海盘车脑苷脂AAC-1、AAC-2的糖基组成相同,均为葡萄糖;长链碱主要由d18∶2、d18∶3以及少量的d22∶1组成;两者结构最主要的差别在脂肪酸部分,AAC-1由C2位不含羟基的脂肪酸组成,而AAC-2则由C2位含1个羟基取代的脂肪酸组成。ESI-MS分析结果表明,AAC-1和AAC-2的分子量范围在740～850amu之间。通过GC-MS和ESI-MS结合使用,可快速推测出多棘海盘车脑苷脂基本结构及单体分子组成,对下一步的构效关系研究具有重要意义。
Abstract：To determine the structure of the cerebrosides in the starfish Asterias amurensis.The cerebrosides isolated from Asterias amurensis through a silica gel column were further analyzed by Gas Chromatography-Mass Spectrometry(GC-MS) and electrospray ionization mass spectrometry(ESI-MS).The GC-MS rusults suggested that the LCBs and glycoside compositions of AAC-1 and AAC-2 are similar but with an obvious difference in their fatty acid composition.Their glycoside composition is glucose while the major LCBs are d18∶2 and d18∶3 together with a few amount of d22∶1.Compared to AAC-1,the fatty acids of AAC-2 were hydroxylated in the C2 position.The ESI-MS spectra showed that molecular weights of AAC-1 and AAC-2 are arised from 740 to 850.The basic structure and monomer molecular composition of the cerebrosids can be confirmed quickly by the methodology,which make sense for the further structure-activity foundation reasearch.
[1]黄韵,余应年,杨军.鞘脂类研究进展[J].浙江大学学报:医学版,2005,34(4):375-379.
[2]Sarah T,Anatoliy B,Kerri H,et al.Biodiversity of sphingoid ba-ses("sphingosines")and related amino alcohols[J].J Lip Res,2008,49:1621-1639.
[3]Natori T,Morita M,Akimoto K,et al.Agelasphins,Novel anti-tumor and immunostimulatory cerebrosides from the marinesponge Agelas mauritianus[J].Tetrahedron,1994,50(9):2771-2784.
[4]Jin Wenzao,Rinehart K L,Jares-Erijman E A.Ophidiacerebro-sides:cytotoxic glycosphingolipids containing a novel sphingosinefrom a sea star[J].J Org Chemistry,1994,59(1):144-147.
[5]Hannun Y A,Obeid L M.Principles of bioactive lipid signaling:les-sons from sphingolipids[J].Nat Rev Mol Cell Biol,2008,9:139-150.
[6]Sugawara T,M Kinoshita,M Ohnishi,et al.Digestion of maizesphingolipids in rats and uptake of sphingadienine by Caco-2cells[J].J Nutr,2003,133:2777-2782.
[7]Sugawara T,M Kinoshita,M Ohnishi,et al.Efflux of sphingoid ba-ses by p-glycoprotein in human intestinal Caco-2cells[J].BiosciBiotechnol Biochem,2004,68:2541-2546.
[8]王长云,顾谦群,周鹏.多棘海盘车用作新型海洋食品原料的可行性研究[J].中国水产科学,1999,6(4):67-71.
[9]Tatsuya S,Tsuyoshi T,Saeko Y,et al.Intestinal absorption ofdietary maize glucosylceramide in lymphatic duct cannulated rats[J].J Lip Res,2010,51:1761-1769.
[10]Rosario Duran,Eva Zubia,Maria J Ortega,et al.New Glu-cosphingolipids from the Ascidian Phallusia fumigata[J].Tet-rahedron,1998,54:14597-14602.
[11]Maria E,Diaz de Vivar,Alicia M Seldes,et al.Two novel glucosylce-ramides from gonads and body walls of the patagonian starfish Allos-tichaster inaequalis[J].Lipids,2002,37(6):597-603.
[12]Yoko O,Takashi T,Satoko A,et al.Squid nerve sphingomyelincontaining an unusual sphingoid base[J].J Lip Res,2000,41:1118-1124.
基本信息:
DOI：10.16441/j.cnki.hdxb.2011.12.011
中图分类号:O657.63;R284
引用信息:
[1]丛培旭,李兆杰,徐杰,等.气相色谱质谱联用及电喷雾质谱法分析多棘海盘车的脑苷脂结构[J].中国海洋大学学报(自然科学版),2011,41(12):63-68.DOI:10.16441/j.cnki.hdxb.2011.12.011.
基金信息:
海洋公益性行业科研专项(201105029);; 国际科技合作项目(2010DFA31330);; “泰山学者”建设工程专项资助
2011-12-15
2011-12-15