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为了解决海藻病害中条件致病菌难以有效分离与鉴定的问题，本研究采用了传统微生物分离培养方法，建立了细菌-海带侵染实验体系，并运用16S rRNA基因序列比对技术，从健康的海带成熟孢子体上分离出一株条件致病菌MS-3。侵染实验及显微观察显示，实验室条件下，菌株MS-3侵染6 h时，可引起海带绿烂的病症。MS-3菌落为圆形，呈亮黄色，菌体呈棒状，具有单根极生鞭毛。经16S rRNA基因序列比对发现，菌株MS-3与新喀里多尼亚弧菌NC470(Vibrio neocaledonicus NC470)的相似度为99.7%。结合菌株MS-3的生理生化特征，将该菌株鉴定为新喀里多尼亚弧菌MS-3(Vibrio neocaledonicus MS-3)。本研究结果不仅丰富了海带条件致病菌的菌种资源，也为后续探索条件致病菌的致病机制及开发海带病害诊断技术奠定了坚实基础。
Abstract：The pathogenic bacteria of seaweed diseases are mostly the opportunistic pathogenic bacteria. In this study, an opportunistic pathogenic bacterium MS-3 was isolated and identified from healthy mature sporophytes of Saccharina japonica by combining the traditional culture-dependent method, bacteria-S. japonica infection assay and 16s rRNA gene sequence comparison. Through the infection assay and microscopic observations, it was shown that strain MS-3 can cause green-rotten diseased symptom in S. japonica after infected at 6 h under laboratory condition. The colony of MS-3 was round and bright yellow. The cells of MS-3 were rod shaped with a single polar flagellum. MS-3 had the highest similarity of 99.7% with Vibrio neocaledonicus NC470 by comparing 16s rRNA gene sequences. Combining the physiological and biochemical characteristics of MS-3, we identified it as Vibrio neocaledonicus MS-3. Our results not only enrich the strain resource of opportunistic pathogenic bacteria of S. japonica and lay a foundation for investigating the pathogenic mechanisms of the opportunistic pathogenic bacteria, but also help to develop the diagnostic technology for S. japonica diseases.
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基本信息:
DOI：10.16441/j.cnki.hdxb.20230132
中图分类号:S946.11
引用信息:
[1]高欣,蔡玲,张吉美,等.一株诱导海带绿烂病的条件致病菌——新喀里多尼亚弧菌MS-3的分离与鉴定[J].中国海洋大学学报(自然科学版),2025,55(04):71-80.DOI:10.16441/j.cnki.hdxb.20230132.
基金信息:
国家自然科学基金项目(42076106)资助~~