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为探究海南省万宁市老爷海人工红树林湿地固碳细菌群落结构及其环境驱动机制，本研究以光滩与红树林地土壤为研究对象，综合运用高通量测序技术与荧光定量PCR分析方法开展系统研究。研究表明：除个别光滩采样点(如GT2)外，红树林地土壤固碳细菌基因丰度总体高于光滩。Chao1物种丰富度指数显示红树林地微生物物种丰富度更优；而Simpson多样性指数、Shannon-Wiener多样性指数和Pielou均匀度指数均呈现空间异质性，表现为在距海较远的样点，光滩的多样性高于红树林地；而在距海较近的样点，则红树林地高于光滩。固碳优势菌门主要为变形菌门(Pseudomonadota)和蓝细菌门(Cyanobacteriota),优势菌属占比：贪铜菌属(Cupriavidus)占比16.11%～24.66%,硫杆菌属(Thiobacillus)占比10.39%～12.77%,硫杆状菌属(Sulfuricaulis)占比9.13%～10.54%;且两类生境存在29个标志差异物种，分属于α-变形菌纲、β-变形菌纲、γ-变形菌纲。主坐标分析(Principal coordinates analysis, PCoA)结合相似性分析(Analysis of similarities, ANOSIM)、置换多因素方差分析(Permutational multivariate analysis of variance, PERMANOVA)显示，湿地固碳细菌群落结构存在明显不同，组间差异大于组内差异。冗余分析(Redundance analysis, RDA)表明：光滩生境固碳细菌群落结构主要受有机碳、易氧化有机碳、粉粒、细砂、有机质、全氮、全磷、黏粒、含水率、pH等因子驱动，红树林地生境则以有机碳、黏粒、易氧化有机碳、有机质、全磷、粉粒、细砂、含水率为关键影响因子，其中土壤粒度与有机碳是两类生境的共同主导因子。研究结果显示，人工种植红树林可通过改善土壤理化性质重塑固碳细菌群落结构与多样性，在滨海湿地生态修复与碳汇功能提升中展现出重要的应用潜力与实践价值。
Abstract：To investigate the community structure of carbon-fixing bacteria and their environmental driving mechanisms in the artificial mangrove wetlands of Laoyehai Lagoon, Wanning City, Hainan Province, we systematically analyzed the soils from bare tidal flats and mangrove areas using high-throughput sequencing and quantitative real-time PCR(qPCR). The results showed that the gene abundance of carbon-fixing bacteria in mangrove soil was generally higher than that in bare tidal flats, except for individual bare tidal flat sampling sites(e.g., GT2). The Chao1 species richness index indicated the superior microbial species richness in mangrove areas, while the Simpson diversity index, Shannon-Wiener diversity index and Pielou evenness index all exhibited spatial heterogeneity. The bare tidal flats far from the ocean are higher than the mangrove areas, whereas mangrove areas close to the ocean had higher values than bare tidal flats. The dominant carbon-fixing phyla were mainly Pseudomonadota and Cyanobacteriota, and the proportions of dominant genera were Cupriavidus(16.11%～24.66%), Thiobacillus(10.39%～12.77%) and Sulfuricaulis(9.13%～10.54%). Additionally, there were 29 marker differential species between the two habitats, which belonged to α-Proteobacteria, β-Proteobacteria and γ-Proteobacteria. Principal Coordinate Analysis(PCoA) combined with Analysis of Similarities(ANOSIM) and Permutational Multivariate Analysis of Variance(PERMANOVA) tests revealed significant differences in the community structure of wetland carbon-fixing bacteria. The differences between groups were greater than those within groups. Redundancy analysis(RDA) demonstrated that the community structure of carbon-fixing bacteria the bare tidal flat habitat was mainly driven by factors such as organic carbon, easily oxidizable organic carbon, silt, fine sand, organic matter, total nitrogen, total phosphorus, clay, water content and pH, while the mangrove areas was mainly driven by organic carbon, clay, easily oxidizable organic carbon, organic matter, total phosphorus, silt, fine sand and water content, among them soil particle size and organic carbon were the common dominant factors in the two habitats. The results of this study confirmed that artificial planting of mangroves can reshape the community structure and diversity of carbon-fixing bacteria by improving soil physicochemical properties, thus demonstrating the important application potential and practical value in coastal wetland ecological restoration and carbon sink function enhancement.
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基本信息:
DOI：10.16441/j.cnki.hdxb.20250204
中图分类号:X171.1;X144
引用信息:
[1]赖郑霞,吴丹,赵志忠,等.海南岛老爷海人工红树林湿地固碳细菌群落特征研究[J].中国海洋大学学报(自然科学版),2026,56(05):32-43.DOI:10.16441/j.cnki.hdxb.20250204.
基金信息:
海南省院士创新平台科研专项项目(YSPTZX202024)资助~~
2025-07-07
2025
2025-12-03
2026-03-18
2026
1
2026-04-27
2026-04-27