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1999, 01,
鲈鱼苗烂鳃、烂尾病病原菌的研究
基金项目(Foundation): 淡水生态和生物技术国家重点实验室(FEBL)项目,联合国教科文组织(UNESCO)资助,国家自然科学基金
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DOI: 10.16441/j.cnki.hdxb.1999.01.014
发布时间: 1999-01-15
出版时间: 1999-01-15
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摘要:

1997年4~5月,对花鲈(Lateolabraxjaponicus)鱼苗期细菌性烂鳃和烂尾病的病原菌进行了研究。从具有明显症状的病鱼的病灶组织分离到2株致病菌C-1和W-1,经人工感染及从人工感染发病鱼体再分离的W-4和W-5菌株的再感染试验结果表明,所分离的细菌为鲈鱼苗烂尾,烂鳃病的致病菌。经64项生物学特性,生理生化特征测试结果表明,这4株菌可归为一类,同属于鳗弧菌(Vibrioanguilarum)。药敏试验结果表明,痢特灵、CIN100、MEL25、呋喃妥因、氯霉素、磺胺药+TMP和美洛西林等7种药物对病原菌有显著的抑制作用。

关键词:
Abstract:

A serious epidemic of seaperch (Lateolabrax japonicus) fry occurred in some fish farms in Laizhou, Shandong Province. The typical symptoms of the diseased fry fish were poor swimming activities, darker body colour, anorexia or even rejection of food, congestive eyes, rotted gills and rotted caudal fins. A rod shaped bacterium was isolated from the diseased fish which has been proved to be the causative agent of the present infection of seaperch fry after experimental infection tests. Sixty four tests of morphological, physiological and biochemical characteristics of 4 isolated and reisolated strains were tested. All the 4 strains were rod shaped, motile with monotrichous flagella, gram negative, non luminescent. The bacteria grew well as faint yellow round colonies on TCBS agar. They were sensitive to vibristatic agent O/129 (150μg/mL), positive to Oxidase and Oxidation/Fermentation tests. They were classified to the same phenon by numerical taxonomy analysis and identified as V.anguillarum. Meanwhile, the sensitivity of two present strains to 47 chemotherapeutants were also investigated. Both strains had resistance to 22 kinds of chemotherapeutants and were more sensitive to Furazolidone, Cinoxacin, Mecillinam, Nitrofurantoin, Chloramphenicol, Sulphamethoxazole/Trimethoprim and Mezlocillin. Acknowledgements The authors thank FEBL, UNESCO and NSFC for their support of this research under contracts 9711B2(FEBL), 861.359.8 (UNESCO) and 39870581 (NSFC)

参考文献

1殷战,徐伯亥.鱼类细菌性疾病的研究.水生生物学报,1995,1(1):76~832李跃祖,华鼎可译.鱼病学教程.英RobertsRJ主编,北京:农业出版社,1984.215~2173AustinB,AustinDA.BacterialFishPathogens:Diseaseinfarmedandwildfish.ElisHorwood,Chichester,(UK)1987,2664麦克法丁JE.医学细菌生化试验鉴定手册.林万明译.北京:北京人民卫生出版社,1985.94~1005WestPAandColwelRR..IdentificationandClasificationofVibrionaceae-AnOverview.In:ColwelRRed.Vibriointheenvironment.NewYork:JohnWiley&SonsInc,1984.285~3636LemosML,ToranzoAEandBarjaJL.Modifiedmediumfortheoxidation-fermentationtestintheidentificationofmarinebacteria.AppliedAndEnvironmentalMicrobiology,49(6):1541~15437张纪忠.微生物分类学(MicrobialTaxonomy),上海:复旦大学出版社,1990.55~618许兵,等.对虾病原菌抑菌药物的研究.青岛海洋大学学报,1993,23(2):43~519BaumannP,etal.GenusVibrio.In:HoltJG.Bergey'sManualofSystematicBacteriology:Vol1.Baltimore:Wiliams&Wilkins,1984.518~53810MercedesAandAnicentRB.AsetofkysforbiochemicalidentificationofenvironmentalVibrioSpecies.JournalofAppliedBacteriology,1994,76:79~8511刘秀珍,邹晓理.海水网箱养殖石斑鱼病原菌研究.热带海洋,1994,1:7~9

基本信息:

DOI:10.16441/j.cnki.hdxb.1999.01.014

中图分类号:S941.42

引用信息:

[1]肖慧,李军,王祥红,纪伟尚,徐怀恕.鲈鱼苗烂鳃、烂尾病病原菌的研究[J].青岛海洋大学学报(自然科学版),1999(01).DOI:10.16441/j.cnki.hdxb.1999.01.014.

基金信息:

淡水生态和生物技术国家重点实验室(FEBL)项目,联合国教科文组织(UNESCO)资助,国家自然科学基金

发布时间:

1999-01-15

出版时间:

1999-01-15

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