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2025 03 v.55 23-33
三种不同芽孢杆菌对凡纳对虾生长、非特异性免疫及抗病力影响的比较研究
基金项目(Foundation): 国家重点研究发展计划项目(2023YFD2401705,2020YFD0900200)资助~~
邮箱(Email):
DOI: 10.16441/j.cnki.hdxb.20240028
中文作者单位:

海水养殖教育部重点实验室(中国海洋大学);三亚热带水产研究院;

摘要(Abstract):

为评估饲料中添加3种不同芽孢杆菌属益生菌对凡纳对虾(Penaeus vannamei)生长性能、非特异性免疫及抗病力的影响,在基础饲料中分别添加1×10~9 CFU·kg-1浓度的枯草芽孢杆菌(Bacillus subtilis)BSXE-1601(BD组)、贝莱斯芽孢杆菌(B.velezensis)26.3(BV组)和蜡样芽孢杆菌(B.cereus)BCXE-01(CD组)投喂对虾,以基础饲料投喂组为对照组,进行了为期6周的养殖实验。养殖实验结束后,进行14 d的副溶血弧菌(Vibrio parahaemolyticus)攻毒实验。研究表明,BD组和BV组对虾末体质量、特定生长率和饲料效率显著高于对照组(P<0.05),其中BV组最高。3个处理组对虾血清中过氧化物酶、总超氧化物歧化酶、酸性磷酸酶和酚氧化酶活性均显著高于对照组(P<0.05),BV组对虾血清中酸性磷酸酶、碱性磷酸酶和总超氧化物歧化酶活性显著高于BD组(P<0.05)。与对照组相比,BD和BV组对虾肝胰腺中proPO、LZM、SOD、HSP70、Imd、Toll、TOR、4E-BP和eIF4E1α基因相对表达量均显著上调(P<0.05)。CD组对虾肝胰腺proPO、LGBP、Imd、Toll、4E-BP和eIF4E2基因相对表达量均显著高于对照组(P<0.05)。BV组对虾肝胰腺SOD、LGBP、Toll和4E-BP基因相对表达量显著高于CD和BD组(P<0.05)。副溶血弧菌攻毒实验结果表明,与对照组相比,3个处理组凡纳对虾累计死亡率均显著降低(P<0.05)。本研究表明,添加枯草芽孢杆菌BSXE-1601和贝莱斯芽孢杆菌26.3均可显著提高凡纳对虾的生长性能,二者对机体免疫反应的诱导作用优于蜡样芽孢杆菌BCXE-01,3种芽孢杆菌均可显著提高凡纳对虾对副溶血弧菌的抵抗力。

关键词(KeyWords): 生长;非特异性免疫;抗病力;副溶血弧菌;芽孢杆菌;凡纳对虾
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基本信息:

DOI:10.16441/j.cnki.hdxb.20240028

中图分类号:S968.22

引用信息:

[1]田相利,李明静,郭泽阳等.三种不同芽孢杆菌对凡纳对虾生长、非特异性免疫及抗病力影响的比较研究[J].中国海洋大学学报(自然科学版),2025,55(03):23-33.DOI:10.16441/j.cnki.hdxb.20240028.

基金信息:

国家重点研究发展计划项目(2023YFD2401705,2020YFD0900200)资助~~

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