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为了评估在人工微颗粒饲料中添加扇贝肽(Scallop peptide)对大黄鱼(Larimichthys crocea)稚鱼的生长性能、摄食相关基因表达、消化酶活性、抗氧化力和炎症基因表达的影响。本实验以初始体质量(8.16±0.84) mg的大黄鱼稚鱼为研究对象，在饲料中分别添加0%、0.05%、0.1%和0.2%的扇贝肽，共配制4种等氮(粗蛋白54.15%)等脂(粗脂肪16.52%)的人工微颗粒饲料，进行为期30 d的摄食生长实验。研究表明，饲喂0.1%扇贝肽饲料稚鱼的FBL(终末体长)、FBW(终末体质量)和SGR(特定生长率)显著高于对照组(P<0.05)。在摄食相关基因表达方面，与对照组的相比，人工微颗粒饲料中添加0.1%扇贝肽可显著提高大黄鱼稚鱼的npy(神经肽Y)和ghrelin(饥饿素)的mRNA表达量，相应地，当稚鱼被饲喂含有0.1%扇贝肽的饲料时，稚鱼的trh(促甲状腺激素释放激素)和leptin(瘦素)的mRNA表达量显著降低(P<0.05)。在消化酶活性方面，饲喂0.1%扇贝肽饲料稚鱼的胰腺段和肠段的胰蛋白酶活性显著高于对照组，同时饲喂0.05%扇贝肽饲料的稚鱼胰腺段的胰蛋白活性也显著高于对照组(P<0.05)。在肠道发育方面，与对照组相比，在人工微颗粒饲料中添加0.1%扇贝肽可以显著提高稚鱼肠道刷状缘的LAP(亮氨酸氨基肽酶)和AKP(碱性磷酸酶活性)活性(P<0.05)。在抗氧化力方面，与对照组相比，人工微颗粒饲料中添加0.1%扇贝肽可显著提高稚鱼内脏团SOD(超氧化物歧化酶)活性，且降低稚鱼内脏团丙二醛(MDA)的含量(P<0.05)。饲喂0.1%扇贝肽饲料稚鱼的内脏团总一氧化氮合酶(T-NOS)活性和诱导型一氧化氮合酶(iNOS)活性显著高于对照组(P<0.05)。在炎症基因表达方面，与对照组相比，添加0.1%和0.2%扇贝肽饲料可显著降低稚鱼的il-1β(白介素-1β)、il-6(白介素-6)和il-8(白介素-8)的mRNA表达量(P<0.05)。综上所述，在人工微颗粒饲料中添加0.1%扇贝肽可以增强促食因子的mRNA表达量、抑制厌食因子的mRNA表达量、增强消化酶活性、提高抗氧化力及缓解炎症反应，最终提高大黄鱼稚鱼的生长性能。
Abstract：A 30-day feeding trial was conducted to determine the effects of dietary scallop peptide supplementation on survival, growth performance, expression of feeding-related genes, activities of digestive enzymes, antioxidant capacity and expression of inflammatory factors of large yellow croaker larvae with an initial weight of(8.16±0.84) mg. Four 54.15% crude protein and 16.52% crude lipid diets were formulated with supplementation of 0%, 0.05%, 0.1% and 0.2% SP, respectively. Results indicated that larvae fed diets with 0.1% SP had higher specific growth rate than the control(P<0.05). Compared with the control, the mRNA expression of orexigenic factor genes including npy and ghrelin were significantly increased in larvae fed the diet with 0.1% SP, while the mRNA expression of anorexigenic factor genes including trh and leptin were significantly decreased in larvae fed the diet with 0.1% SP(P<0.05). The trypsin activity in larvae fed the diet with 0.1% SP was significantly higher than the control, while larvae fed the diet with 0.05% SP also had significantly higher trypsin activity than the control(P<0.05). The LAP and AKP in larvae fed the diet with 0.1% SP was significantly higher than the control(P<0.05). A clear increase of SOD was observed in larvae fed the diet with 0.1% SP compared with the control(P<0.05). Correspondingly, larvae fed the diet with 0.1% SP had significantly lower malondialdehyde(MDA) than the control. The total nitric oxide synthase(T-NOS) and inducible nitric oxide synthase(iNOS) of larvae fed the diet with 0.1% SP was significantly higher than the control. Moreover, the mRNA expression of interleukin-1β(il-1β), interleukin-6(il-6) and interleukin-8(il-8)(pro-inflammatory genes) in larvae fed diets with 0.1% and 0.2% SP were significantly lower than the control(P<0.05). In conclusion, the supplementation of 0.1% SP could enhance the expression of orexigenic factor genes, activities of digestive enzymes and antioxidant capacity, ultimately improving the growth performance of large yellow croaker larvae.
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基本信息:
DOI：10.16441/j.cnki.hdxb.20240304
中图分类号:S965.322
引用信息:
[1]艾庆辉,王云涛,张健敏,等.微颗粒饲料中添加扇贝肽对大黄鱼稚鱼生长性能、消化酶活性和抗氧化力的影响[J].中国海洋大学学报(自然科学版),2024,54(10):145-156.DOI:10.16441/j.cnki.hdxb.20240304.
基金信息:
现代农业产业技术体系项目(CARS-47-11)资助~~
2024-08-29
2024
2024-09-12
2024
1
2024-09-26
2024-09-26