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研究褐藻寡糖在低温下对烟草的保护作用,探讨褐藻寡糖作用机理,将褐藻寡糖溶液均匀喷施到烟草叶面后置于(4±1)℃下进行低温胁迫,间隔不同时间检测相关指标。研究发现,浓度是影响褐藻寡糖抗冻性能的重要因素。0.05%,0.20%和0.30%褐藻寡糖具有诱导激发因子作用,能够诱导烟草CAT、SOD和POD活力提高,清除体内产生的氧自由基,保护细胞膜和叶绿素结构,减少烟草叶片损伤,提高烟草耐低温能力,其中以0.20%褐藻寡糖诱导效果最好;0.10%褐藻寡糖具有抗冻保护剂作用,在24h内烟草各项指标均没有明显变化,可保护细胞免受低温伤害,但48h时冻伤指标增强,其保护作用减弱或者消失;高浓度1.00%褐藻寡糖具有毒副作用,在烟草细胞表面形成较强渗透势,胞内物质外渗严重,烟草细胞结构受到破坏,加速低温下烟草叶片损伤。
Abstract：The objective of the investigation was to test the effects of alginate-derived oligosaccharide (ADO) on the low temperature resistance of tobacco leaves. Different concentrations of ADO were sprayed homogeneously on the surface of tobacco leaves and the tobacco plants were put into (4±1)℃ for low temperature stress. The results showed that concentration was the most important factor, which affected the protection ability of ADO. Compared to the concentration 0, 0.05%, 0.20% and 0.30% ADO solutions all showed chilling resistance inducing abilities and could promote the activities of CAT, SOD and POD markedly in a short time, protect the cell membrane and chlorophylls, reduce the damage of tobacco cells. The 0.20% ADO exhibited the best low temperature resistance inducing ability. The 0.10% ADO had no inducing ability but could be used as a chilling resistance protector in 24 h. After chilling stress for 48 h, the protection effect trailed off and the damage of the tobacco leaves increased sharply. The 1.00% ADO solution had side effects which could form osmotic potential on the surface of tobacco leaves and increase the damage of the tobacco leaves at low temperatures.
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基本信息:
DOI：10.16441/j.cnki.hdxb.2009.02.013
中图分类号:S572
引用信息:
[1]刘瑞志,江晓路,管华诗.褐藻寡糖激发子诱导烟草抗低温作用研究[J].中国海洋大学学报(自然科学版),2009,39(02):243-248.DOI:10.16441/j.cnki.hdxb.2009.02.013.
基金信息:
山东省自主创新项目“应用集成技术进行几种特殊海藻生物产品的产业化开发”;; 国家自然科学基金项目(30771646)资助
2009-03-15
2009-03-15