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2025 04 v.55 39-48
mTOR信号通路对凡纳滨对虾生长、抗氧化及非特异性免疫力的影响
基金项目(Foundation): 国家重点研究发展计划项目(2023YFD2400601); 国家海水鱼产业技术体系项目(CARS-47-G10)资助~~
邮箱(Email): xuanwang@ouc.edu.cn;
DOI: 10.16441/j.cnki.hdxb.20240080
中文作者单位:

海水养殖教育部重点实验室(中国海洋大学);

摘要(Abstract):

为探究mTOR信号通路对凡纳滨对虾(Litopenaeus vannamei)生长、抗氧化及非特异性免疫力的影响,设计了3组等氮等能的配合饲料,分别命名为对照组(Con组)、亮氨酸组(Leu组)和雷帕霉素组(Rap组),在Con组中添加了1%的丙氨酸(Ala),在Leu组中添加了1%的亮氨酸,在Rap组中添加了0.003%的雷帕霉素。选取初始体质量(0.70±0.01)g的凡纳滨对虾,在室内循环水系统中进行养殖实验,每个处理组3个重复,每个重复60尾虾,养殖周期为56 d。养殖结束后,对生长、抗氧化及非特异性免疫力相关指标进行分析。研究显示,Leu组的特定生长率极显著高于Con组(P<0.01),Rap组的特定生长率极显著低于Con组(P<0.01)。对虾肌肉S6蛋白的磷酸化水平在摄食后1 h激活程度最强,然后逐渐减弱。与Con组相比,Leu组摄食后1 h肌肉S6蛋白的磷酸化水平显著上升,Rap组摄食后1 h肌肉S6蛋白的磷酸化水平显著下降(P<0.05)。在对虾血淋巴中,Leu组的碱性磷酸酶、酚氧化酶活力显著高于Con组(P<0.05),Rap组的酸性磷酸酶活力显著低于Con组(P<0.05)。在对虾肝胰腺中,Leu组的过氧化氢酶、总抗氧化酶和酚氧化酶活力显著高于Con组(P<0.05),Leu组的丙二醛含量显著低于Rap组(P<0.05)。Leu组的Lysozyme、C-MnSOD、Lectin相对表达量显著高于Con组(P<0.05),Rap组的Relish、Hsp70相对表达量显著低于Con组(P<0.05)。研究结果表明,饲料中添加亮氨酸可有效激活mTOR信号通路,从而提升凡纳滨对虾的生长性能、抗氧化及非特异性免疫能力;而添加雷帕霉素可有效抑制mTOR信号通路,对凡纳滨对虾生长性能、抗氧化及免疫功能起到抑制作用。

关键词(KeyWords): mTOR信号通路;凡纳滨对虾;亮氨酸;雷帕霉素;生长;抗氧化;非特异性免疫
参考文献

[1] Guertin D A,Sabatini D M.Defining the role of mTOR in cancer[J].Cancer Cell,2007,12(1):9-22.

[2] Saxton R A,Sabatini D M.mTOR signaling in growth,metabolism,and disease[J].Cell,2017,168(6):960-976.

[3] Kim E,Goraksha-Hicks P,Li L,et al.Regulation of TORC1 by rag GTPases in nutrient response[J].Nature Cell Biology,2008,10(8):935-945.

[4] Wilson R P.Amino acids and proteins[M]//Fish Nutrition.[S.l.]:Academic Press,2003:143-179.

[5] Zhang Y,Wang P,Lin S,et al.mTORC1 signaling-associated protein synthesis in porcine mammary glands was regulated by the local available methionine dependingonmethionine sources[J].Amino Acids,2018,50:105-115.

[6] Ahmad I,Ahmed I,Fatma S,et al.Role of branched-chain amino acids on growth,physiology and metabolism of different fish species:A review[J].Aquaculture Nutrition,2021,27(5):1270-1289

[7] Zhou Z,Wang X,Wu X,et al.Effects of dietary leucine levels on growth,feed utilization,neuro-endocrine growth axis and TOR-related signaling genes expression of juvenile hybrid grouper (Epinephelus fuscoguttatus ♀×Epinephelus lanceolatus ♂)[J].Aquaculture,2019,504:172-181.

[8] Xia Y,Sun M,Xie Y,et al.mTOR inhibition rejuvenates the aging gingival fibroblasts through alleviating oxidative stress[J].Oxidative Medicine and Cellular Longevity,2017,2017:6292630.

[9] 陈永康,郑朝中,章双,等.黑水虻幼虫粉对凡纳滨对虾生长、免疫和脂质代谢的影响[J].水生生物学报,2023,47(2):269-278.Chen Y K,Zheng C Z,Zhang S,et al.Effects of larval meal of Tabanus melanopter on growth,immunity and lipid metabolism of Litopenaeus vannamei [J].Chinese Journal of Hydrobiology,2023,47(2):269-278.

[10] Wei Z L,Zhuang Y T,Liu X T,et al.Leucine promotes protein synthesis of juvenile white shrimp Litopenaeus vannamei through TOR signaling pathway[J].Aquaculture,2023(564):739060

[11] 田原,刘成栋,王旋,等.低蛋白饲料添加亮氨酸、精氨酸对大菱鲆幼鱼生长、消化、免疫及mTOR信号通路的影响[J].中国海洋大学学报(自然科学版),2024,54(5):33-43.Tian Y,Liu C D,Wang X,et al.Effects of low protein diet supplemented with leucine and arginine on growth,digestion,immunity and mTOR signaling pathway of juvenile Turbot[J].Periodical of Ocean University of China,2019,54(5):33-43.

[12] Wang Q,He G,Mai K,et al.Chronic rapamycin treatment on the nutrientutilizationand metabolism of juvenile turbot (Psetta maxima)[J].Scientific Reports,2016,6(1):28068.

[13] Cunniff P.Official methods of analysis of AOAC International,method 923.03[J].Trends in Food Science & Technology,1995,6(11):382.

[14] Bos C,Metges C C,Gaudichon C,et al.Postprandial kinetics of dietary amino acds are the main determinant of their metabolism after soy or milk protein ingestion in humans[J].Journal of Nutrition,2003,133(5):1308-1315.

[15] Dickinson J M,Fry C S,Drummond M J,et al.Mammalian target of rapamycin complex 1 activation is required for the stimulation of human skeletal muscle protein synthesis by essential amino acids[J].Journal of Nutrition,2011,141(5):856-862.

[16] Seiliez I,Stéphane P,Lansard M,et al.Dietary carbohydrate-to-protein ratio affects TOR signaling and metabolism-related gene expression in the liver and muscle of rainbow trout after a single meal[J].AJP Regulatory Integrative and Comparative Physiology,2011,300(3):733-743.

[17] Song F,Xu D,Mai K,et al.Comparative study on the cellular and systemicnutrientsensing and intermediary metabolism after partial replacementof fishmeal by meat andbone meal in the diet of turbot (Scophthalmus maximus L.)[J].PLoS One,2016,11(11):e0165708.

[18] Liu J,Mai K,Xu W,et al.Effects of dietary glutamine on survival,growth performance,activities of digestive enzyme,antioxidant status and hypoxia stress resistance of half-smooth tongue sole (Cynoglossus semilaevis Günther) post larvae[J].Aquaculture,2015,446:48-56.

[19] Zou T,Cao S P,Xu W J,et al.Effects of dietary leucine levels on growth,tissue protein content and relative expression of genes related toproteinsynthesis in juvenile gibel carp (Carassius auratus gibelio var.CAS Ⅲ)[J].Aquaculture Research,2018,49(6):2240-2248.

[20] Tan X,Lin H,Huang Z,et al.Effects of dietary leucine on growth performance,feed utilization,non-specific immune responses and gut morphology of juvenile golden pompano Trachinotus ovatus[J].Aquaculture,2016,465:100-107.

[21] Kim D H,Sabatini D M.Raptor and mTOR:Subunits of a nutrient-sensitive complex[J].Current Topics in Microbiology and Immunology,2004(279):259-270.

[22] Liu F,Liu Y,Tian L,et al.Quantitative dietary isoleucine requirement of juvenile Pacific white shrimp,Litopenaeus vannamei (Boone) reared in low-salinity water[J].Aquaculture International,2014,22(4):1481-1497.

[23] López N,Sánchez J,Picó C,et al.Dietary l-leucine supplementation of lactating rats results in a tendency to increase lean/fat ratio associated to lower orexigenic neuropeptide expression in hypothalamus[J].Peptides,2010,31(7):1361-1367.

[24] Zhang Q,Li F,Zhang X,et al.cDNA cloning,characterization and expression analysis of the antioxidant enzyme gene,catalase,of Chinese shrimp Fenneropenaeus chinensis[J].Fish & Shellfish Immunology,2008,24(5):584-591.

[25] Duan Y,Liu P,Li J,et al.Molecular responses of calreticulin gene to Vibrio anguillarum and WSSV challenge in the ridgetail white prawn Exopalaemon carinicauda[J].Fish & Shellfish Immunology,2014,36(1):164-171.

[26] Reddy,Naik M G,Annappaswamy.Evaluation of the dietary essentiality of vitamins for Penaeus monodon[J].Aquaculture Nutrition,1999,5(4):267-275.

[27] 尹森.镜鲤酸性磷酸酶和碱性磷酸酶的QTL分析[D].上海:上海海洋大学,2012.Yin S.QTL Analysis of Acid Phosphatase and Alkaline Phosphatase in Mirror Carp(Cyprinus carpio)[D].Shanghai:Shanghai Ocean University,2012.

[28] Song S K,Beck B R,Kim D,et al.Prebiotics as immunostimulants in aquaculture:A review[J].Fish & Shellfish Immunology,2014,40(1):40-48.

[29] Lu J,Zhang X,Zhou Q,et al.Dietary DL-methionyl-DL-methionine supplementation could improve growth performance under low fishmeal strategies by modulating TOR signalling pathway of Litopenaeus vannamei[J].Aquaculture Nutrition,2021,27(6):1921-1933.

[30] Fang Y Z,Yang S,Wu G,et al.Free radicals,antioxidants and nutrition[J].Nutrition,2002,18(10):872-879.

[31] Nappi A J,Christensen B M.Melanogenesis and associated cytotoxic reactions:Applications to insect innate immunity[J].Insect Biochemistry and Molecular Biology,2005,35(5):443-459.

[32] Cerenius L,Lee B L,S?derh?ll K.The proPO-system:Pros and cons for its role in invertebrate immunity[J].Trends in Immunology,2008,29(6):263-271.

[33] Daniels C L,Merrifield D L,Boothroyd D P,et al.Effect of dietary Bacillus spp.and mannan oligosaccharides (MOS) on European lobster (Homarus gammarus L.) larvae growth performance,gut morphology and gut microbiota[J].Aquaculture,2010,304(1-4):49-57.

[34] Giri S S,Sen S S,Chi C,et al.Effect of dietary leucine on the growth parameters and expression of antioxidant,immune,and inflammatory genes inthe head kidney of Labeo rohita fingerlings[J].Vet Immunol Immunopathol,2015,167(1-2):36-43.

[35] Jiang W D,Deng Y P,Liu Y,et al.Dietary leucine regulates the intestinal immune status,immune-related signalling molecules and tight junctiontranscript abundance ingrass carp (Ctenopharyngodon idella)[J].Aquaculture,2015,444:134-142.

[36] Wu C,Chen L,Lu Z,et al.The effects of dietary leucine on the growthperformances,bodycomposition,metabolic abilities and innate immune responses in black carp Mylopharyngodon piceus[J].Fish & Shellfish Immunology,2017,67:419-428.

[37] Huo Y W,Jin M,Sun P,et al.Effect of dietary leucine on growth performance,hemolymph and hepatopancreas enzyme activities of swimming crab,Portunus trituberculatus[J].Aquaculture Nutrition,2017,23(6):1341-1350.

[38] Johansson M W,S?derh?ll K.The prophenoloxidase activating system andassociated proteins in invertebrates[J].Invertebrate Immunology,1996,15:46-66.

[39] Alexander J B,Ingram G A.Noncellular nonspecific defence mechanismsof fish[J].Annual Review of Fish Diseases,1992,2(1):249-279.

[40] Yao C L,Wu C G,Xiang J H,et al.The lysosome and lysozyme response in Chinese shrimp Fenneropenaeus chinensis to Vibrio anguillarum and laminarin stimulation[J].Journal of Experimental Marine Biology and Ecology,2008,363(1-2):124-129.

[41] Fattman C L,Schaefer L M,Oury T D.Extracellular superoxide dismutase in biology and medicine[J].Free Radical Biology and Medicine,2003,35(3):236-256.

[42] Dodd R B,Drickamer K.Lectin-like proteins in model organisms:Implications for evolution of carbohydrate-binding activity[J].Glycobiology,2001,11(5):71-79.

[43] 张翔,王祖峰.分子伴侣Hsp70变构机制及其在水生动物中的应用研究进展[J].大连海洋大学学报,2022,37(4):12.Zhang X,Wang Z F.Research progress on allosteric mechanism of chaperone Hsp70 and its application in aquatic animals[J].Journal of Dalian Ocean University,2022,37(4):12.

[44] Huang Y H,Kumar R,Liu C H,et al.A novel C-type lectin LvCTL 4.2 has antibacterial activity but facilitates WSSV infection in shrimp (Litopenaeus vannamei)[J].Developmental & Comparative Immunology,2022,126:104239.

[45] Liu G Y,Sabatini D M.mTOR at the nexus of nutrition,growth,ageing and disease[J].Nature Reviews Molecular Cell Biology,2020,21(4):183-203.

[46] Seiliez I,Gabillard J C,Skiba-Cassy S,et al.An in vivo and in vitro assessment of TOR signaling cascade in rainbow trout (Oncorhynchus mykiss)[J].American Journal of Physiology-Regulatory Integrative and Comparative Physiology,2008,295(1):329-335.

基本信息:

DOI:10.16441/j.cnki.hdxb.20240080

中图分类号:S917.4

引用信息:

[1]王馨聆,王旋,刘成栋等.mTOR信号通路对凡纳滨对虾生长、抗氧化及非特异性免疫力的影响[J].中国海洋大学学报(自然科学版),2025,55(04):39-48.DOI:10.16441/j.cnki.hdxb.20240080.

基金信息:

国家重点研究发展计划项目(2023YFD2400601); 国家海水鱼产业技术体系项目(CARS-47-G10)资助~~

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