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为探讨在低温胁迫下条斑紫菜(Neopyropia yezoensis)的生长及光合生理生化响应，本实验以条斑紫菜叶状体为研究对象，测定2℃低温处理条件下条斑紫菜的相关光合生理指标、光合色素含量以及日生长率。研究表明，条斑紫菜叶状体在低温胁迫下生长变缓，野生样品在短期(4 d)胁迫下日生长率(DGR)为1.59%·d-1～3.77%·d-1,长期(20 d)胁迫后DGR为2.19%·d-1～4.12%·d-1;苗期条斑紫菜在低温胁迫(2℃)前2 d时DGR为3.75%·d-1,处理4 d时DGR为8.79%·d-1,经过6 d胁迫后DGR为7.19%·d-1。此外在低温胁迫下条斑紫菜的光合能力也减弱，野生样品在短期(4 d)低温胁迫下，Fv/Fm(PSⅡ最大光化学量子产量)、ΦPSⅡ(PSⅡ实际光化学量子产量)和qP(光化学淬灭系数)均降低，NPQ(非光化学淬灭系数)则升高；经过长期(20 d)胁迫后，Fv/Fm仍显著降低，ΦPSⅡ和qP上升，NPQ降至最低。苗期条斑紫菜在低温胁迫下前2 d Fv/Fm、ΦPSⅡ、NPQ和qP均降低，胁迫4 d时Fv/Fm、ΦPSⅡ、NPQ和qP上升，胁迫6 d时各荧光参数与4 d时相比无显著变化。苗期条斑紫菜低温胁迫6 d后，叶绿素a、藻蓝蛋白含量显著下降(P<0.05),藻红蛋白含量极显著下降(P<0.01),别藻蓝蛋白含量极显著上升(P<0.01)。研究结果表明，条斑紫菜在光合生理及生化上响应低温胁迫并最终影响其生长速率，且随着低温胁迫时间的延长表现出一定的适应性。本研究为探讨条斑紫菜对低温胁迫的适应机制和筛选耐低温良种提供数据参考。
Abstract：In order to explore the growth performance and photosynthetic physiological and biochemical responses of Neopyropia yezoensis to low temperature stress, we took the thallus of N. yezoensis as the studying material, and measured the relevant photosynthetic physiological indexes, photosynthetic pigment content and daily growth rate of N. yezoensis at 2 ℃. The results showed that the growth of N. yezoensis thallus slows down under low temperature stress, and the daily growth rate(DGR) of wild samples ranges from 1.59%·d-1 to 3.77%·d-1 in 4 days and from 2.19%·d-1 to 4.12%·d-1 in 20 days. At the seedling stage, the DGR was 3.75%·d-1 in 2 days ahead of stressing, 8.79%·d-1 in 4 days after stressing, and 7.19%·d-1 in 6 days after stressing. Simultaneously, the photosynthetic capacity decreased, and Fv/Fm(maximum photochemical quantum yield of PSⅡ), ΦPSⅡ(actual photochemical quantum yield of PSⅡ) and qP(photochemical quenching coefficient) of wild samples decreased in 4 days under low temperature stress while NPQ(non-photochemical quenching coefficient) increased. After stressing for 20 days, Fv/Fm still decreased significantly and ΦPSⅡand qP increased while NPQ decreased to the lowest. Fv/Fm, ΦPSⅡ, NPQ and qP decreased in the first 2 days under low temperature stress, and Fv/Fm, ΦPSⅡ, NPQ and qP increased in 4 days of stressing. There was no significant change in fluorescence parameters in 6 days of stressing in comparison with those in 4 days. After 6 days of low temperature stressing at the seedling stage, the content of chlorophyll a and phycocyanin significantly decreased(P<0.05), the content of phycoerythrin significantly decreased(P<0.01), and the content of allophycocyanin significantly increased(P<0.01). The results showed that the photosynthetic physiology and biochemistry of N. yezoensis respond the low temperature stressing and eventually its growth rate is influenced, showing certain adaptability with the extension of low temperature stressing. Our findings provided a data reference for exploring the adaptation mechanism of N. yezoensis to low temperature stress and screening the low temperature resistant varieties.
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基本信息:
DOI：10.16441/j.cnki.hdxb.20230169
中图分类号:S917.3
引用信息:
[1]谢聪颖,钟雪峰,乌兰,等.低温胁迫下条斑紫菜的生长和光合生理生化响应[J].中国海洋大学学报(自然科学版),2024,54(08):33-42.DOI:10.16441/j.cnki.hdxb.20230169.
基金信息:
国家重点研究发展计划项目(2022YFD2400105,2020YFD0900702)资助~~