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从青岛近岸海域筛选到1株产胞外表面活性物质的菌株,可将发酵液的表面张力降至30.5 mN/m,经初步鉴定属于节杆菌属(Arthrobacter sp.)。薄层层析、红外光谱及色质联用分析结果表明,该菌株所产表面活性剂为糖脂类化合物,其化学组成为海藻糖和含β-羟基的十四及十六烷酸。该糖脂类生物表面活性剂具有良好的表面活性、乳化能力,其临界胶束浓度较低,为48.5 mg/L。通过摇瓶发酵实验确定其产表面活性剂的适宜条件为:葡萄糖为碳源,尿素为氮源,培养基初始pH为7.50,培养温度为35℃,培养时间为20 h。
Abstract：A strain was isolated from offshore seawater of Qingdao and identified as Arthrobacter sp.This strain could produce a kind of extracellular surfactant,which could decrease the surface tension of the culture broth to 30.5 mN/m.The results of TCL,IR and GC-MS analyses showed that the biosurfactant was a sort of glycolipid composed of trehalose and two different fatty acids(β-hydroxy tetradecanoic acid and hexadecanoic acid containing).The trehalose lipid,whose critical micelle concentration(CMC) was as low as 48.5 mg/L,had a fine emulsification capacity and high surface-activity.The optimum conditions for its growth and production of biosurfactants were established as follows: glucose as carbon source,urea as nitrogen,initial culture pH value as 7.50,cultivation temperature as 35 ℃ and cultivation period as 20 h.
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基本信息:
DOI：10.16441/j.cnki.hdxb.2009.s1.074
中图分类号:TQ423
引用信息:
[1]朱生凤,朱有庆,辛宇,等.一株分离自海洋的细菌产表面活性剂的研究[J].中国海洋大学学报(自然科学版),2009,39(S1):415-420.DOI:10.16441/j.cnki.hdxb.2009.s1.074.
基金信息:
山东省自然科学基金(Y2008B28);; 中国海洋学与中海石油环保服务有限公司联合攻关项目(2006004)
2009-08-15
2009-08-15