王茹,姜媛媛,林川,潘琳,管守德,于广利,吕友晶,李国云

• 1:中国海洋大学海洋药物教育部重点实验室
• 2:山东省糖科学与糖工程重点实验室
• 3:医药学院
• 4:中国海洋大学物理海洋教育部重点实验室

摘要(Abstract)：

本文以南海海域鹅颈藤壶(Lepas anatifera)为原料，提取鹅颈藤壶背甲与肌肉质柄中的多糖，基于其基本理化性质的分析，比较不同生长时期鹅颈藤壶N-糖链表达谱的差异性。经过脱脂、碱水解/蛋白酶解、乙醇沉淀方法提取得到粗多糖；利用高效液相色谱法、高效凝胶渗透色谱法、苯酚-硫酸法、考马斯亮蓝法对其单糖组成、分子量分布、总糖含量及蛋白质含量进行分析；采用液质分析(PGC-ESI-MS/MS)方法对鹅颈藤壶成体(A-GB)与幼体(L-GB)肌肉质柄中N-糖链进行分析并比较。表明肌肉质柄中多糖分子量为18.40 kDa;总糖含量为11.99%,蛋白质含量60.14%,富含甘露糖、葡萄糖、氨基半乳糖，其中甘露糖的含量高达80%以上。在鹅颈藤壶成体与幼体中均鉴定得到26条N-糖链，成体中高甘露糖型N-糖链占总糖链的20.81%,且相对丰度较高的糖型组成为F_1H_3N_2(39.06%)、F_1H_3N_3(24.44%)、H_3N_3(9.35%);幼体中高甘露糖型N-糖链占总糖链的52.17%,相对丰度较高的糖型组成为F_1H_3N_2(21.65%)、H_6N_2(15.93%)、H_5N_2(14.75%)。鹅颈藤壶多糖是一类N-糖基化的蛋白复合物，推测N-糖链在鹅颈藤壶长发育过程中发挥着至关重要的作用，可能高甘露糖型N-糖链与其附着黏附能力密切相关。

关键词(KeyWords)： 鹅颈藤壶多糖;N-糖链;高甘露糖型;表达谱究

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(31600646);; 泰山学者攀登计划项目(TSPD20210304);; 山东省重大科技创新工程项目(2021ZDSYS22)资助~~

作者(Author): 王茹,姜媛媛,林川,潘琳,管守德,于广利,吕友晶,李国云

DOI: 10.16441/j.cnki.hdxb.20220457

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