张京京,徐冬雪,宋文琦,夏斌,高勤峰

• 1:海水养殖教育部重点实验室(中国海洋大学)
• 2:青岛农业大学海洋科学与工程学院

摘要(Abstract)：

为了探索高温胁迫下刺参(Apostichopus japonicus)H3K9乙酰化水平的变化，研究组蛋白乙酰转移酶(Histone acetyltransferases, HATs)和去乙酰化酶(Histone deacetylases, HDACs)对刺参H3K9乙酰化的影响。本研究通过Western Blot技术研究高温胁迫条件下刺参肠道组织H3K9乙酰化特征，并对刺参基因组中4种组蛋白乙酰转移酶基因ajKAT2B、ajKAT5、ajKAT7、ajKAT8和4种组蛋白去乙酰化酶基因ajSIRT1、ajHDAC1、ajHDAC3、ajHDAC4进行结构解析和系统进化树分析；利用qRT-PCR定量研究在高温胁迫后刺参的8种基因表达量的变化模式。研究表明，通过Western Blot发现，在26℃胁迫下，H3K9ac水平在胁迫48 h后明显上升(P<0.05),在96 h后又迅速下降(P<0.05),表明其在高温胁迫下调控基因转录可能起到重要作用。基因结构解析发现HATs和HDACs含有保守的结构域，通过构建基因系统进化树发现其与物种进化树呈一致的趋势，也表现出功能上的高度保守。qRT-PCR结果表明：4种HATs基因在高温胁迫48 h后均有显著的上升(P<0.05),在胁迫96 h后，ajKAT2B和ajKAT8降低至对照组的表达水平，ajKAT5迅速下降至显著低于对照组水平，ajKAT7稍有降低但仍显著高于对照组。4种HDACs基因中，ajSIRT1在高温胁迫6 h后有明显的上升，在胁迫48 h后显著高于对照组(P<0.05),在胁迫96 h后降低至对照组的表达水平；ajHDAC1在高温胁迫6 h后有明显下降(P<0.05),在胁迫48 h后迅速上升至显著高于对照组水平(P<0.05),然后降低至对照组水平；ajHDAC3和ajHDAC4在高温胁迫6 h后均显著上升(P<0.05),在胁迫48 h后降至对照组水平并继续下降。研究结果表明，刺参H3K9ac乙酰化水平在高温胁迫下表现出明显动态变化，多种HATs和HDACs在mRNA水平上均有明显响应，这可能是导致H3K9ac变化的因素之一。

关键词(KeyWords)： 刺参;高温胁迫;H3K9ac;组蛋白乙酰转移酶;组蛋白去乙酰化酶

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(31902360);; 山东省自然科学基金项目(ZR2022MC050)资助~~

作者(Author): 张京京,徐冬雪,宋文琦,夏斌,高勤峰

DOI: 10.16441/j.cnki.hdxb.20220467

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