管晓伟,王冬梅,陈睿,曲伟华

• 1:中国海洋大学海洋生命学院海洋生物遗传学与育种教育部重点实验室

摘要(Abstract)：

为探究超氧化物歧化酶(Superoxide dismutase, SOD)在条斑紫菜响应温度和失水胁迫时发挥的作用，本文利用生物信息学的方法对条斑紫菜SOD基因家族的进化树、染色体定位、基因结构和蛋白结构等方面进行研究分析。在条斑紫菜中共鉴定出12个SOD基因(PySOD),包括8种Cu/Zn-SOD、3种Mn-SOD和1种Fe-SOD,不均等地分布在3条染色体上，且各含0～2个内含子；SOD基因启动子区域含有不同数目的胁迫响应、生长发育和激素响应元件；Mn-SOD和Fe-SOD二级结构中α螺旋所占比重大，Cu/Zn-SOD中β折叠所占比重大。基于条斑紫菜受到温度和失水胁迫的转录组数据和qPCR结果，PySOD不同胁迫条件下具有不同的表达模式，尤其在失水胁迫时Cu/Zn-SOD表达量呈现不同程度的上调。这种PySOD基因的全基因组分析为了解植物中SOD基因的进化关系提供信息，并且条斑紫菜受温度和失水胁迫时SOD基因的表达分析为改良条斑紫菜育种提供参考。

关键词(KeyWords)： 条斑紫菜;温度胁迫;失水胁迫;超氧化物歧化酶;基因家族

Abstract:

Keywords:

基金项目(Foundation): 山东省自然科学基金项目(ZR2019MC012)资助~~

作者(Author): 管晓伟,王冬梅,陈睿,曲伟华

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