吴磊,涂康,周丽青,刘志鸿,吴彪,孙秀俊

• 1:中国水产科学研究院黄海水产研究所
• 2:青岛海洋科学与技术试点国家实验室海洋渔业科学与食物产出过程功能实验室
• 3:江苏海洋大学海洋科学与水产学院
• 4:莆田市水产研究所

摘要(Abstract)：

为查明温度、盐度和密度对菲律宾蛤仔(Ruditapes philippinarum)稚贝生长和存活的影响，本研究通过响应面法构建了不同环境条件下蛤仔生长存活模型并进行分析。实验设计了3因素(温度、盐度、密度)和3水平(-1、0、1),以稚贝壳长为响应值，探究温度、盐度、密度三因素及其相互作用对蛤仔稚贝生长和存活的影响。研究表明，培育73 d后，温度、盐度、密度的二次效应对蛤仔稚贝的壳长生长影响极显著(P<0.01)。随着温度、盐度、密度的升高，蛤仔壳长生长呈先上升后下降趋势，即表现出抛物线形特征，其中盐度对稚贝的壳长生长影响极显著(P<0.01)。然而，三因子间的交互作用对蛤仔壳长生长的影响均不显著(P>0.05)。壳长预测值Y对应自变量温度(T)、盐度(S)和密度(D)的二次多项回归方程：Y=-6.331 05+0.276 5T+0.573S+0.689D-0.005 66T~2-0.013 7S~2-0.115 9D~2。本研究通过二次响应面回归方程构建了不同环境下蛤仔苗种的生长模型，得出稚贝最适生长环境为温度24.32℃、盐度21.13、密度2.88×10~4粒/m~2,在此条件下蛤仔稚贝平均壳长可达到最大值(4.07 mm)。

关键词(KeyWords)： 菲律宾蛤仔;温度;盐度;密度;生长

Abstract:

Keywords:

基金项目(Foundation): 崂山实验室科技创新项目(LSKJ202203803);; 国家重点研究发展计划项目(2018YFD0900702);; 中国水产科学研究院黄海水产研究所基本科研业务费项目(20603022022001);中国水产科学研究院基本科研业务费项目(2023TD30)资助;; 青岛市市南区科技计划项目(2022-2-026-ZH);; 莆田市科技计划项目(2021NJJ002)~~

作者(Author): 吴磊,涂康,周丽青,刘志鸿,吴彪,孙秀俊

DOI: 10.16441/j.cnki.hdxb.20220337

参考文献(References)：

• [1] 桑士田，闫喜武，杨鹏，等.菲律宾蛤仔稚贝最适生长环境条件的响应面法分析[J].水产学报，2012,36(9):1410-1417.Sang S T,Yan X W,Yang P,et al.Response surface methodology for optimization of growth condition in juvenile Ruditapes philippinarum[J].Journal of Fisheries of China,2012,36(9):1410-1417.
• [2] 李浩浩，于瑞海，杨智鹏，等.温度和盐度对栉江珧受精卵孵化及早期幼虫生长与存活的影响[J].中国海洋大学学报(自然科学版),2017,47(4):22-27.Li H H,Yu R H,Yang Z P,et al.Effects of temperature and salinity on hatching,early larval growth and survival of pen shell Atrina pectinate[J].Periodical of Ocean University of China,2017,47(4):22-27.
• [3] Kong N,Liu X,Li J Y,et al.Effects of temperature and salinity on survival,growth and DNA methylation of juvenile Pacific abalone,Haliotis discus hannai Ino[J].Chinese Journal of Oceanology and Limnology,2017,35(5):1248-1258.
• [4] 许岚，李琪，孔令锋，等.温度和盐度对壳黑长牡蛎幼虫生长和存活的影响[J].中国海洋大学学报(自然科学版),2017,47(8):44-50.Xu L,Li Q,Kong L F,et al.Effects of temperature and salinity on growth and survival of larvae of black-shell pacific oyster (Crassostrea gigas)[J].Periodical of Ocean University of China,2017,47(8):44-50.
• [5] Xu C,Li Q,Chong J D.Combined effect of temperature,salinity,and rearing density on the larval growth of the black shell strain and wild population of the Pacific oyster Crassostrea gigas[J].Aquaculture International,2020,28(1):335-347.
• [6] 张元，朱战豪，姚高友，等.温度和盐度对扇贝“渤海红”稚贝存活和生长的互作效应[J].水产科学，2022,41(1):10.Zhang Y,Zhu Z H,Yao G Y,et al.Interaction effects of temperature and salinity on the survival and growth of juveniles of scallop “Bohai Red”[J].Fisheries Science,2022,41(1):10.
• [7] 宁军号，庞云龙，宋坚，等.偏顶蛤胚胎和幼虫发育及温度对其浮游幼虫生长和发育的影响[J].中国水产科学，2015,22(2):196-203.Ning J H,Pang Y L,Song J,et al.Development of embryo and larva of Modiolus modiolus and effects of temperature on growth and development of the pelagic larva[J].Journal of Fishery Sciences of China,2015,22(2):196-203.
• [8] 廖文崇，朱长波，张汉华，等.水温对香港巨牡蛎摄食和代谢的影响[J].广东农业科学，2011,38(1):7-11.Liao W C,Zhu C B,Zhang H H,et al.Effect of water temperature on the feeding and metabolic physiology of Crassostrea hongkongensis[J].Guangdong Agricultural Sciences,2011,38(1):7-11.
• [9] 白奕天，丁天扬，李东东，等.不同盐度对文蛤(Meretrix meretrix)呼吸代谢及体内酶活性的影响[J].海洋科学，2018,42(6):9.Bai Y T,Ding T Y,Li D D,et al.Effects of different salinity on respiratory metabolism and enzyme activities of Meretrix meretrix[J].Marine Sciences,2018,42(6):9.
• [10] 王尧，曹善茂.盐度对岩扇贝Na+/K+-ATP酶活性的影响[J].科技风，2019(7):241-243.Wang Y,Cao S M.Effects of salinity on activities of Na+/K+-ATP enzymes in rock scallop Crassadoma gigantea[J].Technology Wind,2019(7):241-243.
• [11] Li L,Li Q.Effects of stocking density,temperature,and salinity on larval survival and growth of the red race of the sea cucumber Apostichopus japonicus (Selenka)[J].Aquaculture International,2010,18:447-460.
• [12] 陈志，高如承，胡青，等.温度、盐度和密度交互作用对波纹巴非蛤幼贝生长的影响[J].海洋科学，2013,37(8):55-60.Chen Z,Gao R C,Hu Q,The effect of temperature,salinity and density on growth of Paphia undulata youth analyzed by response surface methodology[J].Marine Sciences,2013,37(8):55-60.
• [13] 郑晓叶，郑天伦，曹飞飞，等.不同密度青蛤养殖塘异养细菌和弧菌的数量变化[J].浙江农业学报，2018,30(10):1647-1654.Zheng X Y,Zheng T L,Cao F F,et al.Quantity dynamics of heterotrophic bacteria and Vibrio in Cyclina sinensis rearing ponds[J].Acta Agriculturae Zhejiangensis,2018,30(10):1647-1654.
• [14] 李长松，房斌，王慧，等.青蛤稚贝放养密度与底质中硫化物相关性研究[J].水产学报，2006,30(6):796-800.Li C S,Fang B,Wang H,et al.Correlativity study on aquiculture density of juvenile clam Cyclina sinensis and concentration of sulfide in sediment[J].Journal of Fisheries of China,2006,30(6):796-800.
• [15] 汪仁官.试验设计及分析[M].北京：中国统计出版社，1998.Wang R G.Experiment Design and Analysis[M].Beijing:China Statistics Press,1998.
• [16] 庄启谦.中国动物志：软体动物门.双売纲.帘蛤科[M].北京：科学出版社，1997.Zhuang Q X.Chinese Zoology:Mollusca.Bivalvia.Veneridae[M].Beijing:Science Press,1997.
• [17] 农业农村部渔业渔政管理局，全国水产技术推广总站，中国水产学会.2021 年中国渔业统计年鉴[M].北京：中国农业出版社，2021.Bureau of Fishery and Fishery Administration,Ministry of Agriculture and Rural Affairs,National Aquatic Technology Promotion Station,China Fisheries Society.2021 China Fisheries Statistics Yearbook[M].Beijing:China Agriculture Press,2021.
• [18] 林笔水，吴天明，黄炳章.温度和盐度对菲律宾蛤仔稚贝生长及发育的影响[J].水产学报，1983,7(1):15-23.Lin B S,Wu T M,Huang B Z.The effects of temperature and salinity on the growth and development of spats of the clam[J].Journal of Fisheries of China,1983,7(1):15-23.
• [19] Yan X W,Zhang G F,Yang F.Effects of diet,stocking density,and environmental factors on growth,survival,and metamorphosis of Manila clam Ruditapes philippinarum larvae[J].Aquaculture,2006,253:350-358.
• [20] 王如才，王昭萍.海水贝类养殖学[M].青岛：中国海洋大学出版社，2008:62-64.Wang R C,Wang Z P.Science of Marine Shellfish Culture[M].Qingdao:China Ocean University Press,2008:62-64.
• [21] 包永波，尤仲杰.几种环境因子对海洋贝类幼虫生长的影响[J].水产科学，2004,12:39-41.Bao Y B,You Z J.Influences of several environmental factors on growth in marine shellfish larvae[J].Fisheries Science,2004,12:39-41.
• [22] Velasco L A,Barros J.Experimental larval culture of the Caribbean scallops Argopecten nucleus and Nodipecten nodosus[J].Aquaculture Research,2008,39(6):603-618.
• [23] 陈丽梅，任一平，徐宾铎.环境因子对胶州湾移植底播菲律宾蛤仔滤水率的影响[J].南方水产，2007,3(2):30-35.Chen L M,Ren Y P,Xu B D.Effects of environmental factors on the clearance rate of the transplant enhanced Manila clam (Ruditapes philippinarum) in the Jiaozhou Bay[J].South China Fisheries Science,2007,3(2):30-35.
• [24] Gruffydd L D,Beaumont A R.A method for rearing Pecten maximus larvae in the laboratory[J].Marine Biology,1972,15(4):350-355.
• [25] 司和，宋志民，唐贤明，等.温度和盐度对波纹巴非蛤胚胎、幼虫及稚贝发育的影响[J].水产科学，2018,37(2):255-258.Si H,Song Z M,Tang X M,et al.Effect of temperature and salinity on development of embryo,larvae and juveniles of clam Paphia undulata[J].Fisheries Science,2018,37(2):255-258.
• [26] 王丹丽，徐善良，尤仲杰，等.温度和盐度对青蛤孵化及幼虫、稚贝存活与生长变态的影响[J].水生生物学报，2005,29(5):495-501.Wang D L,Xu S L,You Z J,et al.The effects of temperature and salinity on the incubation of Cyclina sinensis and survival,growth and metamorphosis of C.sinensis larval and juveniles[J].Acta Hydrobiologica Sinica,2005,29(5):495-501.
• [27] 何苗，周凯，么宗利，等.饵料浓度、温度对缢蛏能量代谢的影响[J].海洋学报(中文版),2017,39(8):129-135.He M,Zhou K,Yao Z L,et al.Effects of algae concentration and temperature on energy metabolism of Sinonovacula constricta[J].Haiyang Xuebao,2017,39(8):129-135.
• [28] 李卫东，吕文刚，王辉，等.温度和盐度对华贵栉孔扇贝幼虫存活联合效应研究[J].水产养殖，2016,37(1):1-8.Li W D,Lv W G,Wang H,et al.Combined effects of temperature and salinity on survival rate of larva in noble scallop,Chlamys nobilis (Reeve)[J].Journal of Aquaculture,2016,37(1):1-8.
• [29] Liu B Z,Dong B,Tang B J,et al.Effect of stocking density on growth,settlement and survival of clam larvae,Meretrix meretrix[J].Aquaculture,2006,258:344-349.
• [30] 沈伟良，尤仲杰，施祥元.温度与盐度对毛蚶受精卵孵化及幼虫生长的影响[J].海洋科学，2009,10(4):5-8.Shen W L,You Z J,Shi X Y.The influence of temperature and salinity on zygote’s hatchingrate and larval growth of Scapharca subcrenata[J].Marine Sciences,2009,10(4):5-8.
• [31] 刘志刚，刘建勇，杨博.温度与盐度对华贵栉孔扇贝幼贝存活与生长的互作效应研究[J].海洋科学，2011,35(10):75-80.Liu Z G,Liu J Y,Yang B.Interactions of temperature and salinity to the survival and growth of Chlamys nobilis (Reeve)[J].Marine Sciences,2011,35(10):75-80.

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