张晓晓,汪多,田相利,董双林

• 1:海水养殖教育部重点实验室(中国海洋大学)

摘要(Abstract)：

以初始体重(1.57±0.04)g的凡纳滨对虾(Litopenaeus vannamei)为实验对象,研究饲料中添加包膜丁酸钠对凡纳滨对虾的生长性能和血清非特异性免疫酶活性的影响。在对虾配合饲料中分别添加0%(对照组)、0.25%、0.5%、1%、2%和3%的包膜丁酸钠作为6种实验饲料,进行投喂,养殖实验进行42d。研究表明,包膜丁酸钠对凡纳滨对虾的生长具有促进作用,各包膜丁酸钠添加组对虾的增重率和特定生长率均显著高于对照组(P<0.05);2%和3%添加组对虾的成活率显著高于对照组(P<0.05);0.5%、1%、2%和3%添加组对虾的饵料系数显著低于对照组(P<0.05)。对于血清非特异性免疫酶活性,1%、2%和3%添加组对虾血清中酚氧化酶(PO)、酸性磷酸酶(ACP)和碱性磷酸酶(AKP)活性均显著高于对照组(P<0.05),血清中超氧化物岐化酶(SOD)活性均与对照组无显著差异(P>0.05);0.5%添加组SOD活性最高,显著高于对照组(P<0.05),与1%、2%和3%添加组无显著差异(P>0.05);0.25%添加组对虾血清中过氧化物酶(POD)活性最高,显著高于对照组和0.5%添加组(P<0.05),与1%、2%和3%添加组无显著差异(P>0.05);1%添加组对虾血清中溶菌酶(LSZ)活性最高,显著高于对照组(P<0.05),与其余添加组无显著差异(P>0.05)。综合上述结果,饲料中添加适量的包膜丁酸钠可以促进凡纳滨对虾的生长,提高血清非特异性免疫酶活性。以特定生长率为评价指标,饲料中包膜丁酸钠的适宜添加量为2.08%。

关键词(KeyWords)： 包膜丁酸钠;生长;非特异性免疫;凡纳滨对虾

Abstract:

Keywords:

基金项目(Foundation): 山东省杰出青年基金项目(JQ201009);; 广东省海洋经济创新发展区域示范专项(GDHY2013-B03-005);; 青岛市民生科技计划项目(15-9-2-96-NSH)资助~~

作者(Author): 张晓晓,汪多,田相利,董双林

DOI: 10.16441/j.cnki.hdxb.20160276

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