不同盐度和培育密度对杂交刺参幼体生长发育的影响Effects of Salinity and Stocking Density on Growth and Development of the Hybrid Larvae of Sea Cucumber
孙秀俊,李琪
摘要(Abstract):
分析了6个不同盐度和5个培育密度对中国刺参和日本红刺参(Apostichopus japonicus)杂交幼体的生长、存活和附着的影响。盐度实验结果显示,受精后第5天,30和35盐度组幼体成活率最高,分别为46.7%和40.0%。第2天至第5天,30盐度组耳状幼体的生长最快,到第5天平均体长达到最大值672.18μm;30盐度组的幼体附着率最高,为17.8%,而15和20盐度组中均没有出现附着的稚参。培育密度实验结果显示,受精后第5天,0.1个/mL密度组的幼体成活率最高,为66.7%,但各密度组的幼体成活率差异均不显著。0.5个/mL密度组幼体生长最快,到第10天达到最大平均体长,为801.38μm;在所有培育密度实验组中,0.5个/mL密度组的幼体附着率最高,为19.1%。因此,为实现单位水体的苗种产量最大化,刺参杂交幼体培育的最佳盐度为30,最适宜的培育密度为0.5个/mL。
关键词(KeyWords): Apostichopus japonicus;杂交;盐度;培育密度;生长
基金项目(Foundation): 山东省科技发展计划项目(2009GG10005013);; 国家科技支撑计划项目(2011BAD13B03)资助
作者(Author): 孙秀俊,李琪
DOI: 10.16441/j.cnki.hdxb.2012.s1.008
参考文献(References):
- [1]Choe S,Oshima Y.On the morphological and ecological differ-ences between two commercial forms,“Green”and“Red”,of theJapanese common sea cucumber,Stichopus japonicus Selenka[J].Nippon Suisan Gakkaishi,1961,27:97-105.
- [2]Kanno M,Kijima A.Quantitative and qualitative evaluation onthe color variation of the Japanese sea cucumber Stichopus japoni-cus[J].Suisanzoshoku,2002,50:63-69.
- [3]胡美燕,李琪,孔令锋,等.中国刺参与日本红刺参杂交子一代的早期生长比较[J].中国海洋大学学报:自然科学版,2009,39:375-380.
- [4]Sun X J,Li Q,Kong L F.Comparative mitochondrial genomicswithin sea cucumber(Apostichopus japonicus):provide new in-sights into relationships among color variants[J].Aquaculture,2010,309:280-285.
- [5]张国范,刘晓,阙华勇,等.贝类杂交及杂种优势理论和技术研究进展[J].海洋科学,2004,28:54-60.
- [6]Gilles R.Osmoregulation in three molluscs:Acanthochitona dis-crepans(Brown),Glycymeris glycymeris(L.)and Mytilus edu-lis(L.)[J].Biol Bull,1972,142:25-35.
- [7]Gilles R.Mechanism of Osmoregulation in Animals[M].NewYork:Wiley Interscience,1979.
- [8]Forcucci D,Lawrence J M.Effect of low salinity on the activity,feeding,growth and absorption efficiency of Luidia clathrata(E-chinodermata:Asteroidea)[J].Mar Biol,1986,92:315-321.
- [9]His E,Robert R,Dinet A.Combined effects of temperature andsalinity on fed and starved larvae of the Mediterranean mussel,Mytilus galloprovincialis and the starved Japanese oyster Cras-sostrea gigas[J].Mar Biol,1989,100:455-463.
- [10]Kashenko S D.Combined effect of temperature and salinity onthe development of the holothurian Eupentacta fraudatrix[J].Russ J Mar Biol,2000,26:188-193.
- [11]Shi Z,Huang X,Fu R,et al.Salinity stress on embryos and ear-ly larval stages of the pomfret Pampus punctatissimus[J].Aq-uaculture,2008,275:306-310.
- [12]Kashenko S D.Desalination effects on the development of theJapanese sea cucumber[J].Biol Morya,1992,3-4:43-52.
- [13]Kashenko S D.Effects of salinity acclimation of the sea cucumberStichopusjaponicuson the adaptive capabilities of its differentstages of development[J].Biol Morya,1997,23:93-100.
- [14]Kashenko S D.Acclimation of the sea cucumber Apostichopusjaponicus to decreased salinity at the blastula and gastrula sta-ges:its effect on the desalination resistance of larvae at subse-quent stages of development[J].Russ J Mar Biol,2000,26:422-426.
- [15]MacDonald B A.Physiological energetics of Japanese scallop Pa-tinopecten yessoensis larvae[J].J Exp Mar Biol Ecol,1988,120:155-170.
- [16]Liu B,Dong B,Tang B,et al.Effect of stocking density ongrowth,settlement and survival of clam larvae,Meretrix mere-trix[J].Aquaculture,2006,258:344-349.
- [17]田传远,李琪,梁英.刺参健康养殖技术[M].青岛:中国海洋大学出版社,2008.
- [18]Velasco L A,Barros J.Experimental larval culture of the Carib-bean scallops Argopecten nucleus and Nodipecten nodosus[J].Aquacult Res,2008,39:603-618.
- [19]Sui S.The progress and prospects of studies on artificial propaga-tion and culture of the sea cucumber,Apostichopus japonicusIn:Lovatelli,A.(Ed.),Advances in sea cucumber aquacultureand management.FAO Fisheries Technical Paper,2004,463:273-276.
- [20]Li L,Li Q.Effects of stocking density,temperature,and salini-ty on larval survival and growth of the red race of the sea cucum-ber Apostichopus japonicus(Selenka)[J].Aquacult Int,2010,18:447-460.
- [21]Berger V J,Kharazova A D.Mechanisms of salinity adaptationsin marine mollusks[J].Hydrobiologia,1997,355:115-126.
- [22]Binyon,J.Physiology of Echinoderms[M].Oxford:PergamonPress,1972.
- [23]Asha,P S,Muthiah P.Effects of temperature,salinity and pHon larval growth,survival and development of the sea cucumberHolothuria spinifera Theel.Aquaculture,2005,250:823-829.
- [24]Fankboner P V.Seasonal Visceral Atrophy and Response to Sa-linity by Parastichopus californicus(Stimpson):Osmoregula-tion[J].SPC Beche-demer Inf Bull,2002:17.
- [25]Vidolin D,Santos-Gouvea IA,Freire CA.Osmotic stability ofthe coelomic fluids of a sea-cucumber(Holothuria grisea)and astarfish(Asterina stellifera)(Echinodermata)exposed to the airduring low tide:a field study[J].Acta Biol Par Curitiba,2002,31(1-4):113-121.
- [26]Li X D,Dong S L,Lei Y Z,et al.The effect of stocking densityof Chinese mitten crab Eriocheir sinensis on rice and crab seedyields in rice-crab culture systems[J].Aquaculture,2007,273:487-493.
- [27]Yan X W,Zhang G F,Yang F.Effects of diet,stocking density,and environmental factors on growth,survival,and metamor-phosis of Manila clamRuditapes philippinarumlarvae[J].Aq-uaculture,2006,253:350-358.
- [28]Raghavan G,Gopinathan C P.Effects of diet,stocking densityand environmental factors on growth,survival and metamorpho-sis of clam,Paphia malabarica(Chemnitz)larvae[J].AquacultRes,2008,39:928-933.
- [29]Ivy G,Giraspy D A B.Development of large scale hatchery pro-duction techniques for the commercially important sea cucumberHolothuria scabra var.versicolour(Conand,1986)inQueensland,Australia[J].SPC Beche-de-mer Inf Bull,2006,24:28-34.
- [30]Laxminarayana A.Induced spawning and larval rearing of the seacucumbers Bohadschia marmorata and Holothuria atrain Mau-ritius[J].SPC Beche-de-mer Inf Bull,2005,22:48-52.
- [31]于东祥,孙慧玲,陈四清,等.海参健康养殖技术[M].北京:海洋出版社,2005.
文章评论(Comment):
|
||||||||||||||||||
|
||||||||||||||||||