姜勇,张学成,孙平楠,陈云松

• 1:中国海洋大学海洋生命学院
• 2:汕头大学医学院

摘要(Abstract)：

根据酵母整合质粒的设计要求,PCR扩增2.2 kb rDNA片段,导入酿酒酵母(Saccharomyces cerevisiae)整合载体pYD1中,设计并合成适合在酵母中表达的鲑鱼降钙素(Salmon Calcitonin),以此为目标基因,构建适合酿酒酵母多拷贝整合的表达载体p-r-sCT。通过载体p-r-sCT引入到酿酒酵母菌株EBY100中,得到酵母重组菌株yAGA2-r-sCT。流式细胞检测结果表明,目标基因鲑鱼降钙素在重组菌株的yAGA2-r-sCT中得到了表达,实现了外源基因在酿酒酵母菌株中的稳定表达。

关键词(KeyWords)： 鲑鱼降钙素;酿酒酵母;rDNA;多拷贝整合;流式细胞技术

Abstract:

Keywords:

基金项目(Foundation): 国家高技术研究发展计划项目(819204210)资助

作者(Author): 姜勇,张学成,孙平楠,陈云松

DOI: 10.16441/j.cnki.hdxb.2009.03.014

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